Teclistamab  (Synonyms: JNJ-7957; JNJ-64007957; Tecvayli)

Teclistamab is a human bispecific antibody to BCMA and CD3 that recognizes BCMA on target cells and CD3 on T cells and induces T cell-mediated cytotoxicity leading to T cell activation and subsequent target cell lysis. Teclistamab can be used in studies of diseases related to multiple myeloma (MM)^[1].

Human IgG4 lambda

Human IgG4 (S228P) lambda1, Isotype Control

Human

CD3 & BCMA

Teclistamab (0.01-1000 nM, 48 h) induces T cell-mediated cytotoxicity with the EC₅₀ values of 0.15 nM in H929 cells, 0.06 nM in MM.1R cell lines, and 0.45 nM in RPMI 8226 cell lines, respectively^[1].
Teclistamab (0.01-1000 nM, 48 h) can bind CD138 ⁺ human BM monocytes in a dose-dependent manner and induces cytotoxicity of human BM monocytes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Teclistamab (i.v., 0.1-1 μg, once every 2 days) has anti-tumor activity at high doses in female NSG (NOD/SCID/γc ^/ ) mice with H929 cells model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

916  [NCBI] & 931  [NCBI]

P07766 & P11836

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

143.64 kDa

2119595-80-9

Liquid

Colorless to light yellow

[Teclistamab]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Human IgG4 lambda

• 
  Immobilized Human CD3 epsilon/CD3e Protein (ECD, His Tag) can bind Teclistamab. The EC₅₀ for this effect is 1.56 ng/mL.
• 
  Immobilized Human BCMA Protein (ECD, His Tag) can bind Teclistamab. The EC₅₀ for this effect is 0.18 ng/mL.
• 
  Flow Cytometry analysis of K562 cells labelling BCMA (red) with Teclistamab (HY-P99392). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG4 lambda (HY-P99987, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Kodandaram Pillarisetti, et al. Teclistamab is an active T cell-redirecting bispecific antibody against B-cell maturation antigen for multiple myeloma. Blood Adv. 2020 Sep 22;4(18):4538-4549.  [Content Brief]