Z-Gly-Gly-Arg-AMC TFA

Cat. No.: HY-P0019B: Ficha de datos Instrucciones de manejo
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Z-Gly-Gly-Arg-AMC TFA is the TFA salt form of Z-Gly-Gly-Arg-AMC (HY-P0019). Z-Gly-Gly-Arg-AMC TFA is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm).

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Z-Gly-Gly-Arg-AMC TFA Estructura química

No. CAS : 66216-79-3

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Other In-stock Forms of Z-Gly-Gly-Arg-AMC TFA:

Z-Gly-Gly-Arg-AMC Z-Gly-Gly-Arg-AMC acetate

Other Forms of Z-Gly-Gly-Arg-AMC TFA:

Z-Gly-Gly-Arg-AMC In-stock
Z-Gly-Gly-Arg-AMC acetate In-stock

11 Publications Citing Use of MCE Z-Gly-Gly-Arg-AMC TFA

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Descripciòn	Z-Gly-Gly-Arg-AMC TFA is the TFA salt form of Z-Gly-Gly-Arg-AMC (HY-P0019). Z-Gly-Gly-Arg-AMC TFA is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm)^[1]^[2].
In Vitro	Guidelines (The following are our recommended protocols. This protocol is for reference only and should be modified according to your specific needs.) 1. Solution preparation^[3] 1.1 Preparation of stock solution Solvent: H2O Concentration: 10 mM. Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing. 1.2 Preparation of working solution Dilute to 10 μM with PBS (or other buffer) (optimized according to the experiment). Note: The working solution should be prepared and used immediately. Keep it away from light. 2. Thrombin inhibition screen 2.1 The samples and thrombin are transferred to a 384-well plate in sequence. 2.2 Add Z-Gly-Gly-Arg-AMC (50 μM) after incubation for 15 minutes. 2.3 Using a buffer containing 10 mM Tris-Cl (pH 7.4), 150 mM NaCl, 10 mM MgCl2, 1 mM CaCl2, 0.1% w/v BSA, 0.01% v/v Triton-X100, and 1% v/v DMSO and the fluorogenic substrate Z-Gly-Gly-Arg-AMC (50 μM). 2.4 The fluorescence intensity is measured with a fluorescence plate reader (Ex/Em = 360/465 nm) at 25°C for a period of 30 min with a read every 3 min. X MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Z-Gly-Gly-Arg-AMC TFA Related Antibodies
Peso molecular	693.63
Fòrmula	C₃₀H₃₄F₃N₇O₉
No. CAS	66216-79-3
Sequence	Z-Gly-Gly-{Arg-AMC}
Sequence Shortening	ZGG-{Arg-AMC}
Envío	Room temperature in continental US; may vary elsewhere.
Almacenamiento	Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación	Ficha de datos (273 KB) Instrucciones de manejo (2659 KB)
Referencias	[1]. Tarandovskiy ID, et al. Antiplatelet agents can promote two-peaked thrombin generation in platelet rich plasma: mechanism and possible applications. PLoS One. 2013;8(2):e55688. [Content Brief] [2]. Machlus KR, et al. Effects of tissue factor, thrombomodulin and elevated clotting factor levels on thrombin generation in the calibrated automated thrombogram. Thromb Haemost. 2009 Nov;102(5):936-44. [Content Brief] [3]. Mothukuri GK, et al. Macrocycle synthesis strategy based on step-wise "adding and reacting" three components enables screening of large combinatorial libraries. Chem Sci. 2020 Jun 26;11(30):7858-7863. [Content Brief]