2. Epigenetics TGF-beta/Smad Apoptosis
  3. PKC Apoptosis
  4. ζ-Stat trisodium

ζ-Stat trisodium  (Synonyms: NSC37044 trisodium)

Cat. No.: HY-123979A Purity: ≥97.0%
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ζ-Stat trisodium (NSC37044 trisodium) is a specific and atypical PKC-ζ inhibitor, with an IC50 of 5 μM. ζ-Stat trisodium can reduce melanoma cell lines proliferation and induce apoptosis, and has antitumor activity in vitro.

For research use only. We do not sell to patients.

ζ-Stat trisodium Chemical Structure

ζ-Stat trisodium Chemical Structure

CAS No. : 31894-34-5

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5 mg USD 360 In-stock
10 mg USD 570 In-stock
50 mg USD 1700 In-stock
100 mg USD 2550 In-stock
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Based on 1 publication(s) in Google Scholar

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ζ-Stat trisodium Related Antibodies

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Description

ζ-Stat trisodium (NSC37044 trisodium) is a specific and atypical PKC-ζ inhibitor, with an IC50 of 5 μM. ζ-Stat trisodium can reduce melanoma cell lines proliferation and induce apoptosis, and has antitumor activity in vitro[1][2].

IC50 & Target[1]

aPKC-ζ

5 μM (IC50)

In Vitro

ζ-Stat (0.1-20 µM) shows only 13% inhibition on PKC-ι at 20 μM, but shows a significant inhibition on PKC-ζ as 51% at 5 μM level[1].
ζ-Stat (0.1-10 µM; 3 d) significantly decreases cell proliferation of SK-MEL-2 and MeWo upon increasing the concentrations[1].
ζ-Stat (7 or 10 µM; 24-72 h) and 5-FU in combination is able to decrease the viability of LoVo CRC cells by more than 75%[2].
ζ-Stat (5 µM; 3 d) shows a significant diminution of phosphorylated, total PKC-ζ, Bcl-2 and PARP levels, and increases Caspase-3 and cleaved-PARP levels in SK-MEL-2 and MeWo cells[1].
ζ-Stat (5 µM; 1-10 h) does not show significant cytotoxicity on MEL-F-NEO, SK-MEL-2 and MeWo cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ζ-Stat trisodium Related Antibodies

Cell Proliferation Assay[1]

Cell Line: MEL-F-NEO, SK-MEL-2 and MeWo cells
Concentration: 0.1, 0.5, 1, 2.5, 5, 7.5, 10 µM
Incubation Time: 3 days
Result: Decreased proliferation by 47.7% for 5 µM in SK-MEL-2 cells and by 50.6% for 5 µM in MeWo cells.
Showed significant inhibitions on MEL-F-NEO cells 19.3% (P ≤ 0.05) at 10 μM.

Western Blot Analysis[1]

Cell Line: SK-MEL-2 and MeWo cells
Concentration: 5 µM
Incubation Time: 3 days
Result: Decreased phosphorylated and total PKC-ζ levels.
Molecular Weight

450.31

Formula

C10H5Na3O10S3
CAS No.
Appearance

Solid

Color

Light yellow to light brown

SMILES

O=S(C1=C2C(O)=CC(S(=O)(O[Na])=O)=CC2=CC(S(=O)(O[Na])=O)=C1)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 12.5 mg/mL (27.76 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2207 mL 11.1035 mL 22.2069 mL
5 mM 0.4441 mL 2.2207 mL 4.4414 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 2.63 mg/mL (5.84 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.2207 mL 11.1035 mL 22.2069 mL 55.5173 mL
5 mM 0.4441 mL 2.2207 mL 4.4414 mL 11.1035 mL
10 mM 0.2221 mL 1.1103 mL 2.2207 mL 5.5517 mL
15 mM 0.1480 mL 0.7402 mL 1.4805 mL 3.7012 mL
20 mM 0.1110 mL 0.5552 mL 1.1103 mL 2.7759 mL
25 mM 0.0888 mL 0.4441 mL 0.8883 mL 2.2207 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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ζ-Stat trisodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ζ-Stat trisodium31894-34-5NSC37044 trisodiumPKCApoptosisProtein kinase CatypicalPKC-ζmelanomaapoptosisantitumorproteinkinaseCInhibitorinhibitorinhibit

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