γ-glutamyltransferase, Porcine kidney  (Synonyms: GGT, Porcine kidney)

γ-glutamyltransferase, Porcine kidney (GGT, Porcine kidney) is an enzyme located on the outer surface of the cell membrane. γ-glutamyltransferase, Porcine kidney maintains the physiological concentration of cytoplasmic glutathione and the cell's defense against oxidative stress by cleaving extracellular glutathione and increasing the availability of amino acids. γ-glutamyltransferase, Porcine kidney is used for pancreatic cancer research^[1][2][3][4].

Iso-peptide hydrolysis
1 μg of tubulin was incubated with 1 U of γ-glutamyltransferase and 10 mM Gly-Gly peptide in 65 μL of 50 mM Tris/Cl, pH 8.0 (containing 150 mM NaCl) at 37 °C for 22 h. γ-glutamyltransferase, Porcine kidney co-incubated with GSAO serves as a positive control to demonstrate the conversion of GSAO to GCAO^[3].
γ-glutamyltransferase, Porcine kidney shows properties similar to those of the renal enzyme in LLC-PK₁ cells, and its activity increases with cell density^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9046-27-9

Solid

White to off-white

[g-glutamyltransferase, Porcine kidney]

2.3.2.2

≥20 U/mg soild

One unit is defined as the amount of enzyme that will release 1.0 μmol of p-nitroaniline from L-γglutamyl-β-nitroaniline per minute at 25°C and pH 8.5

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Whitfield JB. Gamma glutamyl transferase. Crit Rev Clin Lab Sci. 2001 Aug;38(4):263-355.  [Content Brief]

  [2]. G Ndrepepa, et al. "Gamma-glutamyl transferase and cardiovascular disease." Annals of translational medicine 4.24 (2016).

  [3]. Ramsay EE, et al. Employing pancreatic tumor γ-glutamyltransferase for therapeutic delivery. Mol Pharm. 2014 May 5;11(5):1500-11.  [Content Brief]

  [4]. Sepúlveda FV, et al. The development of gamma-glutamyltransferase in a pig renal-epithelial-cell line in vitro. Relationship to amino acid transport. Biochem J. 1982 Nov 15;208(2):509-12.  [Content Brief]