Cat. No.: HY-N0375

18α-Glycyrrhetinic acid

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

18α-Glycyrrhetinic acid, a diet-derived compound, is an inhibitor of NF-kB and an activator of proteasome, which serves as pro-longevity and anti-aggregation factor in a multicellular organism. 18α-Glycyrrhetinic acid induces apoptosis.

For research use only. We do not sell to patients.

Purity: 99.87%
CAS No.: 1449-05-4
Formula: C₃₀H₄₆O₄
Molecular Weight:470.68

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

18α-Glycyrrhetinic acid Chemical Structure

CAS No.: 1449-05-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]^[2]

Proteasome

NF-κB

All NF-κB Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
4T1	IC₅₀	96.22 μM Compound: 2; GA	Cytotoxicity in mouse 4T1 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay Cytotoxicity in mouse 4T1 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay	[PMID: 28754470]
CT26	IC₅₀	70.48 μM Compound: 2; GA	Cytotoxicity in mouse CT26 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay Cytotoxicity in mouse CT26 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay	[PMID: 28754470]
DU-145	IC₅₀	136.4 μM Compound: 2; GA	Cytotoxicity in human DU145 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay Cytotoxicity in human DU145 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay	[PMID: 28754470]
EA.hy 926	IC₅₀	>100 μM Compound: 2; GA	Cytotoxicity in human EAhy926 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity in human EAhy926 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 28754470]
H9c2	IC₅₀	>100 μM Compound: 2; GA	Cytotoxicity in rat H9c2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity in rat H9c2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 28754470]
HCT-116	IC₅₀	75.08 μM Compound: 2; GA	Cytotoxicity in human HCT116 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay Cytotoxicity in human HCT116 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay	[PMID: 28754470]
HCT-8	IC₅₀	82.22 μM Compound: 2; GA	Cytotoxicity in human HCT8 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay Cytotoxicity in human HCT8 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay	[PMID: 28754470]
HeLa	IC₅₀	>50 μM Compound: 2; GA	Cytotoxicity in human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity in human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 28754470]
HepG2	IC₅₀	>50 μM Compound: 2; GA	Cytotoxicity in human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity in human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 28754470]
HT-29	IC₅₀	>50 μM Compound: 2; GA	Cytotoxicity in human HT-29 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity in human HT-29 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 28754470]
HT-29	IC₅₀	77.23 μM Compound: 2; GA	Cytotoxicity in human HT-29 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay Cytotoxicity in human HT-29 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay	[PMID: 28754470]
MCF7	IC₅₀	75.66 μM Compound: 2; GA	Cytotoxicity in human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity in human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 28754470]
MDA-MB-231	IC₅₀	101.2 μM Compound: 2; GA	Cytotoxicity in human MDA-MB-231 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay Cytotoxicity in human MDA-MB-231 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay	[PMID: 28754470]
MDA-MB-231	IC₅₀	84.7 μM Compound: 2; GA	Cytotoxicity in human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity in human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 28754470]
MDCK	IC₅₀	>100 μM Compound: 2; GA	Cytotoxicity in human MDCK cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity in human MDCK cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 28754470]
PC-3	IC₅₀	88.04 μM Compound: 2; GA	Cytotoxicity in human PC3 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay Cytotoxicity in human PC3 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay	[PMID: 28754470]
TERT-RPE1	IC₅₀	63.41 μM Compound: 2; GA	Cytotoxicity in human hTERT-RPE1 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity in human hTERT-RPE1 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 28754470]

In Vitro

18α-Glycyrrhetinic acid (18a-GA) markedly reduces LX-2 cell numbers by 14.8% and 31.2% after 48 h and 72 h of treatment, respectively (P< 0.05). 18α-Glycyrrhetinic acid also significantly increases the percentage of LX-2 cells in phase G0/G1 and decreases it in phase S after treated for 48 h and 72 h compare with the control group. 18α-Glycyrrhetinic acid increases apoptosis to 6.8% at 48 h, compare with control (2.5%), and at 72 h the percentages of apoptotic cells in control and the treatment groups are 3.1% and 15.6%, respectively, in LX-2 cells (P<0.01). Furthermore, 18α-Glycyrrhetinic acid induces expression of PPAR-γ and alters some cell cycle and apoptosis-related proteins. 18α-Glycyrrhetinic acid also inhibits NF-κB DNA-binding activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

18α-Glycyrrhetinic acid (18α-GA) treatment significantly enhances life span of C. elegans strains with the most effective concentration being 20 μg/mL. Results reveal a significant delay of paralysis upon 18α-Glycyrrhetinic acid treatment. 18α-Glycyrrhetinic acid treatment also confers a significant reduction of Aβ deposits^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1449-05-4
Appearance Solid
Molecular Weight 470.68
Formula C₃₀H₄₆O₄
Color White to off-white
SMILES

CC1(C)[C@@H](O)CC[C@@]2(C)[C@@]1([H])CC[C@]([C@@](CC[C@]3(C)[C@]4([H])C[C@@](C)(C(O)=O)CC3)(C)C4=C5)(C)[C@]2([H])C5=O
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (4)

All Bio/Physico-chemical AssayCell Proliferation/Viability AssayCell Migration/Invasion Assay

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Journal Impact Factor

NPJ Precis Oncol

Calcium-modulated GJB6-GRHL3 positive feedback loop attenuates ESCC progression through AKT signaling pathway inhibition. [Abstract]

2026 Jun 4. PMID: 42243308

18α-Glycyrrhetinic acid purchased from MedChemExpress. Usage Cited in: NPJ Precis Oncol. 2026 Jun 4. [Abstract]

CCK-8 growth-curve analysis of GJB6-knockdown KYSE30 cells after treatment with 18α-GA (18α-Glycyrrhetinic acid; 10 μM; 1 h). Cell proliferation was partly restored by 18α-GA (10 μM, 1 h) in the GJB6 knockdown group of KYSE30 cells.

18α-Glycyrrhetinic acid purchased from MedChemExpress. Usage Cited in: NPJ Precis Oncol. 2026 Jun 4. [Abstract]

Transwell migration assay with GJIC inhibitor 18α-GA (18α-Glycyrrhetinic acid; 10 μM; 1 h) in GJB6 RNA interfered KYSE30 cells.
Cell Calcium

Drug repurposing DMSO from an old solvent to a new candidate for the treatment of colitis and sepsis in male mice. [Abstract]

2026 May 23:136:103155. PMID: 42235215

18α-Glycyrrhetinic acid purchased from MedChemExpress. Usage Cited in: Cell Calcium. 2026 May 23:136:103155. [Abstract]

Cumulative concentration-response curves, R_max, AUC, and EC₅₀ of DMSO-induced vasorelaxation in arterioles from mice after incubation with 18α-G (18α-Glycyrrhetinic acid; 10 μM; 20 min). 18α-G significantly attenuated DMSO-induced vasorelaxation.
Microvasc Res

Functional heterogeneity of endothelium-dependent vasorelaxation in different order branches of mesenteric artery in female/male mice. [Abstract]

2025 Mar:158:104777. PMID: 39613183

18α-Glycyrrhetinic acid purchased from MedChemExpress. Usage Cited in: Microvasc Res. 2025 Mar:158:104777. [Abstract]

Representative tracings show ACh concentration-dependently induced vasorelaxation of the first-order branch of or second-order branch of male mice mesenteric arteries in the presence of 10 μmol/L 18α-Glycyrrhetinic acid (10 μM; 20 min). ACh-induced vasorelaxation was inhibited by 18α-glycyrrhetinic acid in second but not in first order branch.
chemRxiv

2026 Apr 6.

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (21.25 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1246 mL

10.6229 mL

21.2459 mL

5 mM

0.4249 mL

2.1246 mL

4.2492 mL

10 mM

0.2125 mL

1.0623 mL

2.1246 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 1.14 mg/mL (2.42 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.14 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.4 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.14 mg/mL (2.42 mM); Clear solution

This protocol yields a clear solution of ≥ 1.14 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.4 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[1]

For the determination of cell cycle, 5×10⁵ cells per well are seeded onto 6-well plates and incubated overnight in complete growth medium (DMEM+10% FBS). After starvation for 24 h, the cells are subsequently stimulated with 10% FBS with or without the presence of 18α-Glycyrrhetinic acid (18a-GA). The final concentration of 18α-Glycyrrhetinic acid is 8.0 mM. The cells are incubated for 24 h, 48 h and 72 h, respectively, and then the flow cytometric analysis is performed. Flow cytometric analysis is performed in triplicate. After being treated with 18α-Glycyrrhetinic acid, the cells are stained and then cell apoptosis status is measured by flow cytometry^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[2]

C. elegans strains are used in this study. Synchronized L4 larvae CL2006 animals (100 to 120 animals per condition) are transferred to nematode growth medium (NGM) plates containing either 18α-Glycyrrhetinic acid (18α-GA) or DMSO at 20°C. Synchronized CL4176 animals (150 to 300 animals per condition) are transferred to NGM plates containing either 18α-Glycyrrhetinic acid or DMSO at 16°C for 48 h before temperature upshift to 25°C for transgene induction. Scoring of paralyzed animals is initiated at day 1 of adulthood for CL2006 strain and 24 h after temperature upshift for CL4176 strain. Each paralysis assay is repeated at least thrice. Nematodes are scored as paralyzed if they exhibit halos of cleared bacteria around their heads or fail to undergo half end body wave propagation upon prodding^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zong L et al. 18α-glycyrrhetinic acid extracted from Glycyrrhiza radix inhibits proliferation and promotes apoptosis of the hepatic stellate cell line. J Dig Dis. 2013 Jun;14(6):328-36. [Content Brief]

[2]. Papaevgeniou N, et al. 18α-Glycyrrhetinic Acid Proteasome Activator Decelerates Aging and Alzheimer's Disease Progression in Caenorhabditis elegans and Neuronal Cultures. Antioxid Redox Signal. 2016 Dec 1;25(16):855-869. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1246 mL	10.6229 mL	21.2459 mL	53.1146 mL
	5 mM	0.4249 mL	2.1246 mL	4.2492 mL	10.6229 mL
	10 mM	0.2125 mL	1.0623 mL	2.1246 mL	5.3115 mL
	15 mM	0.1416 mL	0.7082 mL	1.4164 mL	3.5410 mL
	20 mM	0.1062 mL	0.5311 mL	1.0623 mL	2.6557 mL

Keywords

18α-Glycyrrhetinic acid

4 Cited Publications

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18α-Glycyrrhetinic acid

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Publications Citing Use of MedChemExpress (MCE) 18α-Glycyrrhetinic acid

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Protocol

Purity & Documentation

References