2-PMPA (Synonyms: 2-(Phosphonomethyl)pentanedioic acid)

Name: 2-PMPA
Brand: MedChemExpress
SKU: HY-100788
Rating: 5.0 (5 reviews)

Cat. No.: HY-100788 Purity: 98.0%: Data Sheet
COA

SDS
Handling Instructions
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2-PMPA (2-(Phosphonomethyl)pentanedioic acid) is a glutamate carboxypeptidase II (GCPII) inhibitor with an IC₅₀ of 0.0003 μM. 2-PMPA shows low blood-brain barrier penetration. 2-PMPA sodium blocks the hydrolysis of NAAG, regulates glutamate levels in the brain and neurovascular coupling. 2-PMPA is applicable to the research of neurological diseases.

For research use only. We do not sell to patients.

CAS No. : 173039-10-6

Size	Stock	Quantity
Oil + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Oil
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of 2-PMPA:

2-PMPA sodium In-stock
2-PMPA (Standard) Get quote

5 Publications Citing Use of MCE 2-PMPA

Flow Cytometry

Histological Imaging/Staining

: 2-PMPA purchased from MedChemExpress. Usage Cited in: ACS Nano. 2021 Apr 27;15(4):7179-7194. [Abstract]; After incubation with 2-PMPA (0.5 mM; 6 h), the uptake of Fluoro-GBP@Fe₃O₄ (50 μg Fe/mL) in C4-2 cells was assessed by flow cytometry.

: 2-PMPA purchased from MedChemExpress. Usage Cited in: ACS Nano. 2021 Apr 27;15(4):7179-7194. [Abstract]; 2-PMPA (0.5 μM; i.v.; 1-6 h) obviously inhibited the gradual aggregation of GBP@Fe₃O₄ at the tumor sites in C4-2 tumor-bearing mice.

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Biological Activity
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References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
LNCaP	IC₅₀	1.1 nM Compound: 7, 2-PMPA	Inhibition of NAALADase activity in human LNCaP cell membranes assessed as inhibition of [3H]NAG conversion to [3H]glutamate after 30 mins by liquid scintillation counting Inhibition of NAALADase activity in human LNCaP cell membranes assessed as inhibition of [3H]NAG conversion to [3H]glutamate after 30 mins by liquid scintillation counting	[PMID: 23025786]
LNCaP	IC₅₀	52 nM Compound: PMPA	Displacement of [125I]23 from PSMA in human LNCAP cells Displacement of [125I]23 from PSMA in human LNCAP cells	[PMID: 26894427]

In Vitro

2-PMPA remains stable in rat plasma when stored at room temperature for 4 h, subjected to 3 freeze-thaw cycles, stored at -80°C for 28 days, and placed in a 10°C autosampler for 18 h^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

2-PMPA (167-250 mg/kg; intraperitoneal injection; single dose) induces a dose-dependent biphasic BOLD signal response in the gray matter of anesthetized mice, with a sustained signal decrease of 2.15-4.64% at 250 mg/kg and a transient signal decrease of 1% at 167 mg/kg^[1].
2-PMPA (80 mg/kg; i.p.; single dose) exerts no significant effects on motor coordination or cognitive ability in Swiss-Webster mice within 24 hours^[1].
2-PMPA (100 mg/kg; intraperitoneal injection; single dose) is rapidly absorbed in male Wistar rats, with low brain penetration (a brain/plasma AUC_0-t ratio of 0.018), while it shows higher penetration in peripheral nerve tissues^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Swiss-Webster (SW) (male, approx 6-8 wk old)^[2]
Dosage:	80 mg/kg
Administration:	i.p.; single dose
Result:	Showed mean latency to fall not significantly different from control mice at all tested time points (70, 240, 420, 1440 min post-administration). Caused no impairment in motor coordination or cognitive ability observed over the 24-h period.

Molecular Weight

226.12

Formula

C₆H₁₁O₇P

CAS No.

173039-10-6

Appearance

Oil

Color

Off-white to light yellow

SMILES

O=C(O)C(CP(O)(O)=O)CCC(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (442.24 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.4224 mL	22.1122 mL	44.2243 mL
5 mM	0.8845 mL	4.4224 mL	8.8449 mL
10 mM	0.4422 mL	2.2112 mL	4.4224 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (442.24 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.0%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (246 KB) HNMR (247 KB)

Handling Instructions (2659 KB)

References

[1]. Rais R, et al. Bioanalytical method for evaluating the pharmacokinetics of the GCP-II inhibitor 2-phosphonomethyl pentanedioic acid (2-PMPA). J Pharm Biomed Anal. 2014 Jan;88:162-9. [Content Brief]

[2]. Baslow MH, et al. 2-PMPA, a NAAG peptidase inhibitor, attenuates magnetic resonance BOLD signals in brain of anesthetized mice: evidence of a link between neuron NAAG release and hyperemia. J Mol Neurosci. 2005;26(1):1-15. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	4.4224 mL	22.1122 mL	44.2243 mL	110.5608 mL
	5 mM	0.8845 mL	4.4224 mL	8.8449 mL	22.1122 mL
	10 mM	0.4422 mL	2.2112 mL	4.4224 mL	11.0561 mL
	15 mM	0.2948 mL	1.4741 mL	2.9483 mL	7.3707 mL
	20 mM	0.2211 mL	1.1056 mL	2.2112 mL	5.5280 mL
	25 mM	0.1769 mL	0.8845 mL	1.7690 mL	4.4224 mL
	30 mM	0.1474 mL	0.7371 mL	1.4741 mL	3.6854 mL
	40 mM	0.1106 mL	0.5528 mL	1.1056 mL	2.7640 mL
	50 mM	0.0884 mL	0.4422 mL	0.8845 mL	2.2112 mL
	60 mM	0.0737 mL	0.3685 mL	0.7371 mL	1.8427 mL
	80 mM	0.0553 mL	0.2764 mL	0.5528 mL	1.3820 mL
	100 mM	0.0442 mL	0.2211 mL	0.4422 mL	1.1056 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

2-PMPA Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2-PMPA173039-10-62-(Phosphonomethyl)pentanedioic acidCarboxypeptidaseInhibitorinhibitorinhibit

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2-PMPA (Synonyms: 2-(Phosphonomethyl)pentanedioic acid)

Customer Review

Other Forms of 2-PMPA:

2-PMPA Related Antibodies

5 Publications Citing Use of MCE 2-PMPA

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

2-PMPA Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

2-PMPA Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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