2. Stem Cell/Wnt TGF-beta/Smad Apoptosis
  3. PKA Apoptosis
  4. 8-Bromo-cAMP sodium salt

8-Bromo-cAMP sodium salt  (Synonyms: 8-ブロモ-cAMP; 8-Br-Camp sodium salt)

製品番号: HY-12306 純度: 99.91%
COA 取扱説明書 Technical Support

8-Bromo-cAMP sodium (8-Br-Camp) sodium salt, a cyclic AMP analog, is an activator of cyclic AMP-dependent protein kinase (PKA). 8-Bromo-cAMP sodium salt has anti-proliferative and apoptotic effects against cancer cells.

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CAS 番号 : 76939-46-3

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 在庫あり
Solution
10 mM * 1 mL in DMSO USD 55 在庫あり
Solid
5 mg $50 在庫あり
10 mg $70 在庫あり
25 mg $120 在庫あり
50 mg $185 在庫あり
100 mg $280 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 36 publication(s) in Google Scholar

Other Forms of 8-Bromo-cAMP sodium salt:

8-Bromo-cAMP sodium salt 関連抗体

Top Publications Citing Use of Products

顧客検証

Histological Imaging/Staining
Flow Cytometry
WB

    8-Bromo-cAMP sodium salt purchased from MedChemExpress. Usage Cited in: EMBO J. 2024 Nov;43(21):5018-5036.  [Abstract]

    Human ESC cell line (hESCs) was treated with 8-Bromo-cAMP sodium salt (0.5 mM; 1-8 days) plus MPA (1 μM) for different times, and western blotting assay was used to detect the expression of CDKN2A, CDKN1A, TP53 (indicator of cell senescence) and PRL.

    8-Bromo-cAMP sodium salt purchased from MedChemExpress. Usage Cited in: Part Fibre Toxicol. 2022 Feb 17;19(1):13.  [Abstract]

    Leydig cells were incubated with 0.2 mg/mL PS-MPs for 1 h and then cultured in serum-free medium in the presence of 0.1 mM 8-Bromo-cAMP sodium salt (0.1 mM) for another 24 h. The expression of StAR in cells was analyzed by western blotting.

    8-Bromo-cAMP sodium salt purchased from MedChemExpress. Usage Cited in: Sci Total Environ. 2022 Oct 10:842:156854.  [Abstract]

    8-Bromo-cAMP sodium salt (8-Br-cAMP, 100 μM; 2 h) pretreatment significantly elevated both PKA and p-PKA levels in SiNPs-treated AC16 cells.

    8-Bromo-cAMP sodium salt purchased from MedChemExpress. Usage Cited in: Sci Total Environ. 2022 Oct 10:842:156854.  [Abstract]

    8-Bromo-cAMP sodium salt (8-Br-cAMP, 100 μM; 2 h) pretreatment significantly reduced apoptosis of AC16 cells induced by SiNPs.

    8-Bromo-cAMP sodium salt purchased from MedChemExpress. Usage Cited in: Inflammation. 2022 Dec;45(6):2419-2432.  [Abstract]

    Histopathological changes in the colon tissue sections from from Npr1−/− mice treated with 8-Bromo-cAMP sodium salt (8-Br-cGMP, 0.1 mM; i.v.; Once daily for 4 weeks) or saline (n = 4).

    PKA アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    PKA PKAR PKACα

    • 生物活性

    • 純度とドキュメンテーション

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    製品説明

    8-Bromo-cAMP sodium (8-Br-Camp) sodium salt, a cyclic AMP analog, is an activator of cyclic AMP-dependent protein kinase (PKA). 8-Bromo-cAMP sodium salt has anti-proliferative and apoptotic effects against cancer cells[1][2].

    IC50 & Target

    PKA[1]
    体外実験

    8-Bromo-cAMP (0.1/0.5 mM) sodium salt enhances the reprogramming efficiency of human neonatal foreskin fibroblast (HFF1) cells, and 0.1 mM of 8-Bromo-cAMP shows a synergistic effect with Valproic acid (HY-10585) (0.5 mM)[1].
    8-Bromo-cAMP (20 μM, 24 and 48 h) sodium salt induces apoptosis in esophageal cancer cell line (Eca-109)[2].
    8-Bromo-cAMP (0.5 mM, 2 days) sodium salt induces decidualization of human endometrial stromal cells[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    8-Bromo-cAMP sodium salt 関連抗体
    体内実験

    8-Bromo-cAMP (60 mg/kg/day, i.p., 7 days) sodium salt reduces tumor in CT26 tumor mice[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: CT26 tumor mice[4]
    Dosage: 60 mg/kg/day
    Administration: i.p., 7 days
    Result: Decreases amounts of primary CRC tumor nodules and liver metastases.
    Reduces vasculogenic mimicry (PAS–CD31 staining of colorectal and intestinal tumors).
    Inhibits cAMP and VEGF expression, increases expression of PKA in tumor tissues.
    分子量

    430.08

    分子式

    C10H10BrN5NaO6P
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    BrC1=NC2=C(N=CN=C2N)N1[C@H](O[C@@](CO3)([H])[C@@]4([H])OP3(O[Na])=O)[C@@H]4O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    DMSO : ≥ 125 mg/mL (290.64 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : ≥ 100 mg/mL (232.51 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.3251 mL 11.6257 mL 23.2515 mL
    5 mM 0.4650 mL 2.3251 mL 4.6503 mL
    10 mM 0.2325 mL 1.1626 mL 2.3251 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (4.84 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (4.84 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (232.51 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.91%

    COA (250 KB) HNMR (232 KB) LCMS (129 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.3251 mL 11.6257 mL 23.2515 mL 58.1287 mL
    5 mM 0.4650 mL 2.3251 mL 4.6503 mL 11.6257 mL
    10 mM 0.2325 mL 1.1626 mL 2.3251 mL 5.8129 mL
    15 mM 0.1550 mL 0.7750 mL 1.5501 mL 3.8752 mL
    20 mM 0.1163 mL 0.5813 mL 1.1626 mL 2.9064 mL
    25 mM 0.0930 mL 0.4650 mL 0.9301 mL 2.3251 mL
    30 mM 0.0775 mL 0.3875 mL 0.7750 mL 1.9376 mL
    40 mM 0.0581 mL 0.2906 mL 0.5813 mL 1.4532 mL
    50 mM 0.0465 mL 0.2325 mL 0.4650 mL 1.1626 mL
    60 mM 0.0388 mL 0.1938 mL 0.3875 mL 0.9688 mL
    80 mM 0.0291 mL 0.1453 mL 0.2906 mL 0.7266 mL
    100 mM 0.0233 mL 0.1163 mL 0.2325 mL 0.5813 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    8-Bromo-cAMP sodium salt Related Classifications

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    8-Bromo-cAMP76939-46-38-Br-CampPKAApoptosisProtein kinase AHFF1Eca-109endometrial stromal cellInhibitorinhibitorinhibit

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    製品名:
    8-Bromo-cAMP sodium salt
    製品番号:
    HY-12306
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