2. Stem Cell/Wnt TGF-beta/Smad Apoptosis
  3. PKA Apoptosis
  4. 8-Bromo-cAMP

8-Bromo-cAMP  (Synonyms: 8-Br-Camp)

Art. -Nr.: HY-12306A Reinheit: 99.48%
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8-Bromo-cAMP (8-Br-Camp), a cyclic AMP analog, is an activator of cyclic AMP-dependent protein kinase (PKA). 8-Bromo-cAMP has anti-proliferative and apoptotic effects against cancer cells.

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CAS. Nr. : 23583-48-4

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Based on 36 publication(s) in Google Scholar

Other Forms of 8-Bromo-cAMP:

8-Bromo-cAMP Related Antibodies

Top Publications Citing Use of Products

36 Publications Citing Use of MCE 8-Bromo-cAMP

Histological Imaging/Staining
Flow Cytometry
WB

    8-Bromo-cAMP purchased from MedChemExpress. Usage Cited in: EMBO J. 2024 Nov;43(21):5018-5036.  [Abstract]

    Human ESC cell line (hESCs) was treated with 8-Bromo-cAMP sodium salt (0.5 mM; 1-8 days) plus MPA (1 μM) for different times, and western blotting assay was used to detect the expression of CDKN2A, CDKN1A, TP53 (indicator of cell senescence) and PRL.

    8-Bromo-cAMP purchased from MedChemExpress. Usage Cited in: Part Fibre Toxicol. 2022 Feb 17;19(1):13.  [Abstract]

    Leydig cells were incubated with 0.2 mg/mL PS-MPs for 1 h and then cultured in serum-free medium in the presence of 0.1 mM 8-Bromo-cAMP sodium salt (0.1 mM) for another 24 h. The expression of StAR in cells was analyzed by western blotting.

    8-Bromo-cAMP purchased from MedChemExpress. Usage Cited in: Sci Total Environ. 2022 Oct 10:842:156854.  [Abstract]

    8-Bromo-cAMP sodium salt (8-Br-cAMP, 100 μM; 2 h) pretreatment significantly elevated both PKA and p-PKA levels in SiNPs-treated AC16 cells.

    8-Bromo-cAMP purchased from MedChemExpress. Usage Cited in: Sci Total Environ. 2022 Oct 10:842:156854.  [Abstract]

    8-Bromo-cAMP sodium salt (8-Br-cAMP, 100 μM; 2 h) pretreatment significantly reduced apoptosis of AC16 cells induced by SiNPs.

    8-Bromo-cAMP purchased from MedChemExpress. Usage Cited in: Inflammation. 2022 Dec;45(6):2419-2432.  [Abstract]

    Histopathological changes in the colon tissue sections from from Npr1−/− mice treated with 8-Bromo-cAMP sodium salt (8-Br-cGMP, 0.1 mM; i.v.; Once daily for 4 weeks) or saline (n = 4).

    Alle PKA Isoform-spezifische Produkte anzeigen:

    Alle Isoformen anzeigen
    PKA PKAR PKACα

    • Biologische Aktivität

    • Reinheit & Dokumentation

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    Beschreibung

    8-Bromo-cAMP (8-Br-Camp), a cyclic AMP analog, is an activator of cyclic AMP-dependent protein kinase (PKA). 8-Bromo-cAMP has anti-proliferative and apoptotic effects against cancer cells[1][2].

    IC50 & Target

    PKA[1]
    In Vitro

    8-Bromo-cAMP (0.1/0.5 mM) enhances the reprogramming efficiency of human neonatal foreskin fibroblast (HFF1) cells, and 0.1 mM of 8-Bromo-cAMP shows a synergistic effect with Valproic acid (HY-10585) (0.5 mM)[1].
    8-Bromo-cAMP (20 μM, 24 and 48 h) induces apoptosis in esophageal cancer cell line (Eca-109)[2].
    8-Bromo-cAMP (0.5 mM, 2 days) induces decidualization of human endometrial stromal cells[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    8-Bromo-cAMP Related Antibodies
    In Vivo

    8-Bromo-cAMP (60 mg/kg/day, i.p., 7 days) reduces tumor in CT26 tumor mice[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: CT26 tumor mice[4]
    Dosage: 60 mg/kg/day
    Administration: i.p., 7 days
    Result: Decreases amounts of primary CRC tumor nodules and liver metastases.
    Reduces vasculogenic mimicry (PAS–CD31 staining of colorectal and intestinal tumors).
    Inhibits cAMP and VEGF expression, increases expression of PKA in tumor tissues.
    Molekulargewicht

    408.10

    Formel

    C10H11BrN5O6P
    CAS. Nr.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    OP1(O[C@@]2([H])[C@@H](O)[C@@H](O[C@]2([H])CO1)N3C(Br)=NC4=C3N=CN=C4N)=O
    Versand

    Room temperature in continental US; may vary elsewhere.
    Speicherung
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Lösungsmittel & Löslichkeit
    In Vitro: 

    1 M NaOH : 100 mg/mL (245.04 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4504 mL 12.2519 mL 24.5038 mL
    5 mM 0.4901 mL 2.4504 mL 4.9008 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molaritätsrechner

    • Verdünnungsrechner

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Reinheit & Dokumentation

    Purity: 99.48%

    SDS (393 KB)

    COA (250 KB) HNMR (272 KB) RP-HPLC (257 KB) LCMS (87 KB)

    Handling Instructions (2659 KB)
    Verweise

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    1 M NaOH 1 mM 2.4504 mL 12.2519 mL 24.5038 mL 61.2595 mL
    5 mM 0.4901 mL 2.4504 mL 4.9008 mL 12.2519 mL
    10 mM 0.2450 mL 1.2252 mL 2.4504 mL 6.1259 mL
    15 mM 0.1634 mL 0.8168 mL 1.6336 mL 4.0840 mL
    20 mM 0.1225 mL 0.6126 mL 1.2252 mL 3.0630 mL
    25 mM 0.0980 mL 0.4901 mL 0.9802 mL 2.4504 mL
    30 mM 0.0817 mL 0.4084 mL 0.8168 mL 2.0420 mL
    40 mM 0.0613 mL 0.3063 mL 0.6126 mL 1.5315 mL
    50 mM 0.0490 mL 0.2450 mL 0.4901 mL 1.2252 mL
    60 mM 0.0408 mL 0.2042 mL 0.4084 mL 1.0210 mL
    80 mM 0.0306 mL 0.1531 mL 0.3063 mL 0.7657 mL
    100 mM 0.0245 mL 0.1225 mL 0.2450 mL 0.6126 mL
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    8-Bromo-cAMP Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    8-Bromo-cAMP23583-48-48-Br-CampPKAApoptosisProtein kinase AHFF1Eca-109endometrial stromal cellInhibitorinhibitorinhibit

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