8-Bromo-cAMP sodium salt (Synonyms: 8-Br-Camp sodium salt)

Name: 8-Bromo-cAMP sodium salt
Brand: MedChemExpress
SKU: HY-12306
Rating: 5.0 (36 reviews)

Cat. No.: HY-12306 Purity: 99.91%: Data Sheet
COA

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8-Bromo-cAMP sodium (8-Br-Camp) sodium salt, a cyclic AMP analog, is an activator of cyclic AMP-dependent protein kinase (PKA). 8-Bromo-cAMP sodium salt has anti-proliferative and apoptotic effects against cancer cells.

For research use only. We do not sell to patients.

CAS No. : 76939-46-3

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 36 publication(s) in Google Scholar

Other Forms of 8-Bromo-cAMP sodium salt:

8-Bromo-AMP In-stock
8-Bromo-cAMP In-stock

36 Publications Citing Use of MCE 8-Bromo-cAMP sodium salt

Histological Imaging/Staining

Flow Cytometry

: 8-Bromo-cAMP sodium salt purchased from MedChemExpress. Usage Cited in: EMBO J. 2024 Nov;43(21):5018-5036. [Abstract]; Human ESC cell line (hESCs) was treated with 8-Bromo-cAMP sodium salt (0.5 mM; 1-8 days) plus MPA (1 μM) for different times, and western blotting assay was used to detect the expression of CDKN2A, CDKN1A, TP53 (indicator of cell senescence) and PRL.

: 8-Bromo-cAMP sodium salt purchased from MedChemExpress. Usage Cited in: Part Fibre Toxicol. 2022 Feb 17;19(1):13. [Abstract]; Leydig cells were incubated with 0.2 mg/mL PS-MPs for 1 h and then cultured in serum-free medium in the presence of 0.1 mM 8-Bromo-cAMP sodium salt (0.1 mM) for another 24 h. The expression of StAR in cells was analyzed by western blotting.

: 8-Bromo-cAMP sodium salt purchased from MedChemExpress. Usage Cited in: Sci Total Environ. 2022 Oct 10:842:156854. [Abstract]; 8-Bromo-cAMP sodium salt (8-Br-cAMP, 100 μM; 2 h) pretreatment significantly elevated both PKA and p-PKA levels in SiNPs-treated AC16 cells.

: 8-Bromo-cAMP sodium salt purchased from MedChemExpress. Usage Cited in: Sci Total Environ. 2022 Oct 10:842:156854. [Abstract]; 8-Bromo-cAMP sodium salt (8-Br-cAMP, 100 μM; 2 h) pretreatment significantly reduced apoptosis of AC16 cells induced by SiNPs.

: 8-Bromo-cAMP sodium salt purchased from MedChemExpress. Usage Cited in: Inflammation. 2022 Dec;45(6):2419-2432. [Abstract]; Histopathological changes in the colon tissue sections from from Npr1^−/− mice treated with 8-Bromo-cAMP sodium salt (8-Br-cGMP, 0.1 mM; i.v.; Once daily for 4 weeks) or saline (n = 4).

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PKA PKAR PKACα

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

PKA^[1]

In Vitro

8-Bromo-cAMP (0.1/0.5 mM) sodium salt enhances the reprogramming efficiency of human neonatal foreskin fibroblast (HFF1) cells, and 0.1 mM of 8-Bromo-cAMP shows a synergistic effect with Valproic acid (HY-10585) (0.5 mM)^[1].
8-Bromo-cAMP (20 μM, 24 and 48 h) sodium salt induces apoptosis in esophageal cancer cell line (Eca-109)^[2].
8-Bromo-cAMP (0.5 mM, 2 days) sodium salt induces decidualization of human endometrial stromal cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

8-Bromo-cAMP (60 mg/kg/day, i.p., 7 days) sodium salt reduces tumor in CT26 tumor mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	CT26 tumor mice^[4]
Dosage:	60 mg/kg/day
Administration:	i.p., 7 days
Result:	Decreases amounts of primary CRC tumor nodules and liver metastases. Reduces vasculogenic mimicry (PAS–CD31 staining of colorectal and intestinal tumors). Inhibits cAMP and VEGF expression, increases expression of PKA in tumor tissues.

Molecular Weight

430.08

Formula

C₁₀H₁₀BrN₅NaO₆P

CAS No.

76939-46-3

Appearance

Solid

Color

White to off-white

SMILES

BrC1=NC2=C(N=CN=C2N)N1[C@H](O[C@@](CO3)([H])[C@@]4([H])OP3(O[Na])=O)[C@@H]4O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : ≥ 125 mg/mL (290.64 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 100 mg/mL (232.51 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3251 mL	11.6257 mL	23.2515 mL
5 mM	0.4650 mL	2.3251 mL	4.6503 mL
10 mM	0.2325 mL	1.1626 mL	2.3251 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (232.51 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (278 KB) SDS (394 KB)

COA (250 KB) HNMR (232 KB) LCMS (129 KB)

Handling Instructions (2659 KB)

References

[1]. Wang Y, et al. A cyclic AMP analog, 8-Br-cAMP, enhances the induction of pluripotency in human fibroblast cells. Stem Cell Rev. 2011 Jun;7(2):331-41. [Content Brief]

[2]. Wang HM, et al. Dual effects of 8-Br-cAMP on differentiation and apoptosis of human esophageal cancer cell line Eca-109. World J Gastroenterol. 2005 Nov 7;11(41):6538-42. [Content Brief]

[3]. Baek MO, et al. Differential regulation of mTORC1 and mTORC2 is critical for 8-Br-cAMP-induced decidualization. Exp Mol Med. 2018 Oct 30;50(10):1-11. [Content Brief]

[4]. Wang S, et al. Angiogenesis and vasculogenic mimicry are inhibited by 8-Br-cAMP through activation of the cAMP/PKA pathway in colorectal cancer. Onco Targets Ther. 2018 Jul 2;11:3765-3774 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.3251 mL	11.6257 mL	23.2515 mL	58.1287 mL
	5 mM	0.4650 mL	2.3251 mL	4.6503 mL	11.6257 mL
	10 mM	0.2325 mL	1.1626 mL	2.3251 mL	5.8129 mL
	15 mM	0.1550 mL	0.7750 mL	1.5501 mL	3.8752 mL
	20 mM	0.1163 mL	0.5813 mL	1.1626 mL	2.9064 mL
	25 mM	0.0930 mL	0.4650 mL	0.9301 mL	2.3251 mL
	30 mM	0.0775 mL	0.3875 mL	0.7750 mL	1.9376 mL
	40 mM	0.0581 mL	0.2906 mL	0.5813 mL	1.4532 mL
	50 mM	0.0465 mL	0.2325 mL	0.4650 mL	1.1626 mL
	60 mM	0.0388 mL	0.1938 mL	0.3875 mL	0.9688 mL
	80 mM	0.0291 mL	0.1453 mL	0.2906 mL	0.7266 mL
	100 mM	0.0233 mL	0.1163 mL	0.2325 mL	0.5813 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

8-Bromo-cAMP sodium salt Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

8-Bromo-cAMP76939-46-38-Br-CampPKAApoptosisProtein kinase AHFF1Eca-109endometrial stromal cellInhibitorinhibitorinhibit

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8-Bromo-cAMP sodium salt (Synonyms: 8-Br-Camp sodium salt)

Customer Review

Other Forms of 8-Bromo-cAMP sodium salt:

8-Bromo-cAMP sodium salt Related Antibodies

36 Publications Citing Use of MCE 8-Bromo-cAMP sodium salt

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All PKA Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

8-Bromo-cAMP sodium salt Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

8-Bromo-cAMP sodium salt Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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