AkaLumine hydrochloride

Name: AkaLumine hydrochloride
Brand: MedChemExpress
SKU: HY-112641A
Rating: 4.5 (1 reviews)

Cat. No.: HY-112641A Purity: 99.84%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

AkaLumine hydrochloride is a luciferin analogue, with a K_m of 2.06 μM for recombinant Fluc protein.

For research use only. We do not sell to patients.

AkaLumine hydrochloride Chemical Structure

CAS No. : 2558205-28-8

Size	Price	Stock	Quantity
5 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 460	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1820	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 3200	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE AkaLumine hydrochloride

•Stem Cell Res. October 2021, 102532.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

AkaLumine hydrochloride is a luciferin analogue, with a K_m of 2.06 μM for recombinant Fluc protein.

IC₅₀ & Target

Km: 2.06 μM (recombinant Fluc protein)^[1].

In Vitro

In both LLC/luc and MDA-MB-231/luc cells treated with AkaLumine hydrochloride, the signal is maximal at lower concentrations (by 2.5 μM), whereas the bioluminescence generated by D-luciferin and CycLuc1 increase as concentrations increase further, not appearing to reach a maximal signal even at 250 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

AkaLumine hydrochloride displays greatly enhanced signals emanating from metastasis in the lung, as demonstrated by 8.1-fold higher with AkaLumine hydrochloride as compared with D-luciferin administration. To evaluate the advantage of AkaLumine hydrochloride over CycLuc1 in detecting targets in deep tissue, bioluminescence images are compared between AkaLumine hydrochloride- and CycLuc1-treated mice 15 min after intravenous injection of LLC/luc cells at the dose of 5 mM, which is the maximum concentration of CycLuc1 due to its low water solubility. AkaLumine hydrochloride exhibits 3.3-fold increase in detection sensitivity of disseminated cancer cells in the lung compared with CycLuc1. The advantage of AkaLumine hydrochloride is further confirmed by imaging the same mice bearing lung metastasis after intraperitoneal injection of 5 mM substrates in order of CycLuc1 and AkaLumine hydrochloride, and in the inverse order at an 8-h interval. AkaLumine hydrochloride displays about fourfold increase in signals from lung metastasis compared with CycLuc1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

338.85

Formula

C₁₆H₁₉ClN₂O₂S

CAS No.

2558205-28-8

Appearance

Solid

Color

Dark purple to black

SMILES

O=C([C@@H]1N=C(/C=C/C=C/C2=CC=C(N(C)C)C=C2)SC1)O.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (295.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (147.56 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9512 mL	14.7558 mL	29.5116 mL
5 mM	0.5902 mL	2.9512 mL	5.9023 mL
10 mM	0.2951 mL	1.4756 mL	2.9512 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 4 mg/mL (11.80 mM); Clear solution

This protocol yields a clear solution of ≥ 4 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 4 mg/mL (11.80 mM); Clear solution

This protocol yields a clear solution of ≥ 4 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (246 KB) HNMR (146 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

Dyeing Example

Description: AkaLumine hydrochloride can be used to detect Akaluc expression in vitro.
Method: For cell labeling.
1. Disperse and suspend cells by 100 μL PBS in a 96-well plate at a density of 2×10⁵.
2. Add AkaLumine hydrochloride (100 µM) and 5 mM ATP-mg to cells, and use a bioluminescence imaging system to analyze.

References

[1]. Kuchimaru T, et al. A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat Commun. 2016 Jun 14;7:11856. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.9512 mL	14.7558 mL	29.5116 mL	73.7790 mL
	5 mM	0.5902 mL	2.9512 mL	5.9023 mL	14.7558 mL
	10 mM	0.2951 mL	1.4756 mL	2.9512 mL	7.3779 mL
	15 mM	0.1967 mL	0.9837 mL	1.9674 mL	4.9186 mL
	20 mM	0.1476 mL	0.7378 mL	1.4756 mL	3.6889 mL
	25 mM	0.1180 mL	0.5902 mL	1.1805 mL	2.9512 mL
	30 mM	0.0984 mL	0.4919 mL	0.9837 mL	2.4593 mL
	40 mM	0.0738 mL	0.3689 mL	0.7378 mL	1.8445 mL
	50 mM	0.0590 mL	0.2951 mL	0.5902 mL	1.4756 mL
	60 mM	0.0492 mL	0.2459 mL	0.4919 mL	1.2296 mL
	80 mM	0.0369 mL	0.1844 mL	0.3689 mL	0.9222 mL
	100 mM	0.0295 mL	0.1476 mL	0.2951 mL	0.7378 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.