1. Dye Reagents
  2. TokeOni

TokeOni (Synonyms: AkaLumine hydrochloride)

Cat. No.: HY-112641A
Handling Instructions

TokeOni (AkaLumine hydrochloride) is a luciferin analogue, with a Km of 2.06 μM for recombinant Fluc protein.

For research use only. We do not sell to patients.

TokeOni Chemical Structure

TokeOni Chemical Structure

Size Stock
250 mg Get quote
500 mg Get quote

* Please select Quantity before adding items.

Customer Review

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Information

  • Purity & Documentation

  • References

Description

TokeOni (AkaLumine hydrochloride) is a luciferin analogue, with a Km of 2.06 μM for recombinant Fluc protein.

IC50 & Target

Km: 2.06 μM (recombinant Fluc protein)[1].

In Vitro

Interestingly, in both LLC/luc and MDA-MB-231/luc cells treated with TokeOni (AkaLumine hydrochloride), the signal is maximal at lower concentrations (by 2.5 μM), whereas the bioluminescence generated by D-luciferin and CycLuc1 increase as concentrations increase further, not appearing to reach a maximal signal even at 250 μM[1].

In Vivo

TokeOni (AkaLumine hydrochloride) displays greatly enhanced signals emanating from metastasis in the lung, as demonstrated by 8.1-fold higher with TokeOni as compared with D-luciferin administration. To evaluate the advantage of TokeOni (AkaLumine hydrochloride) over CycLuc1 in detecting targets in deep tissue, bioluminescence images are compared between TokeOni- and CycLuc1-treated mice 15 min after intravenous injection of LLC/luc cells at the dose of 5 mM, which is the maximum concentration of CycLuc1 due to its low water solubility. TokeOni exhibits 3.3-fold increase in detection sensitivity of disseminated cancer cells in the lung compared with CycLuc1. The advantage of TokeOni is further confirmed by imaging the same mice bearing lung metastasis after intraperitoneal injection of 5 mM substrates in order of CycLuc1 and AkaLumine hydrochloride, and in the inverse order at an 8-h interval. TokeOni displays about fourfold increase in signals from lung metastasis compared with CycLuc1[1].

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
Molecular Weight

338.85

Formula

C₁₆H₁₉ClN₂O₂S

SMILES

O=C([[email protected]@H]1N=C(/C=C/C=C/C2=CC=C(N(C)C)C=C2)SC1)O.Cl

Shipping

Room temperature in continental US; may vary elsewhere

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number *

 

Organization name *

Country or Region *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
TokeOni
Cat. No.:
HY-112641A
Quantity:

TokeOni

Cat. No.: HY-112641A