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AkaLumine hydrochloride 

Cat. No.: HY-112641A Purity: 96.64%
Handling Instructions

AkaLumine hydrochloride is a luciferin analogue, with a Km of 2.06 μM for recombinant Fluc protein.

For research use only. We do not sell to patients.

AkaLumine hydrochloride Chemical Structure

AkaLumine hydrochloride Chemical Structure

Size Price Stock Quantity
5 mg USD 105 In-stock
Estimated Time of Arrival: December 31
10 mg USD 175 In-stock
Estimated Time of Arrival: December 31
50 mg USD 700 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1200 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Publications Citing Use of MCE AkaLumine hydrochloride

  • Biological Activity

  • Purity & Documentation

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  • Customer Review

Description

AkaLumine hydrochloride is a luciferin analogue, with a Km of 2.06 μM for recombinant Fluc protein.

IC50 & Target

Km: 2.06 μM (recombinant Fluc protein)[1].

In Vitro

In both LLC/luc and MDA-MB-231/luc cells treated with AkaLumine hydrochloride, the signal is maximal at lower concentrations (by 2.5 μM), whereas the bioluminescence generated by D-luciferin and CycLuc1 increase as concentrations increase further, not appearing to reach a maximal signal even at 250 μM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

AkaLumine hydrochloride displays greatly enhanced signals emanating from metastasis in the lung, as demonstrated by 8.1-fold higher with AkaLumine hydrochloride as compared with D-luciferin administration. To evaluate the advantage of AkaLumine hydrochloride over CycLuc1 in detecting targets in deep tissue, bioluminescence images are compared between AkaLumine hydrochloride- and CycLuc1-treated mice 15 min after intravenous injection of LLC/luc cells at the dose of 5 mM, which is the maximum concentration of CycLuc1 due to its low water solubility. AkaLumine hydrochloride exhibits 3.3-fold increase in detection sensitivity of disseminated cancer cells in the lung compared with CycLuc1. The advantage of AkaLumine hydrochloride is further confirmed by imaging the same mice bearing lung metastasis after intraperitoneal injection of 5 mM substrates in order of CycLuc1 and AkaLumine hydrochloride, and in the inverse order at an 8-h interval. AkaLumine hydrochloride displays about fourfold increase in signals from lung metastasis compared with CycLuc1[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

338.85

Formula

C₁₆H₁₉ClN₂O₂S

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (147.56 mM; Need ultrasonic)

DMSO : 25 mg/mL (73.78 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9512 mL 14.7558 mL 29.5116 mL
5 mM 0.5902 mL 2.9512 mL 5.9023 mL
10 mM 0.2951 mL 1.4756 mL 2.9512 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 5 mg/mL (14.76 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 5 mg/mL (14.76 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: 96.64%

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Product Name:
AkaLumine hydrochloride
Cat. No.:
HY-112641A
Quantity:
MCE Japan Authorized Agent: