AkaLumine hydrochloride
Based on 7 publication(s) in Google Scholar
AkaLumine hydrochloride is a D-luciferin (HY-12591A) analogue with a Km of 2.06 μM for recombinant firefly luciferase (Fluc) protein. AkaLumine hydrochloride emits near-infrared (NIR) light (λmax=677 nm) in reactions with native Fluc. AkaLumine hydrochloride has high tissue-penetration and increases detection sensitivity from deep-tissue targets.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 2558205-28-8
- Formula: C16H19ClN2O2S
- Molecular Weight:338.85
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) AkaLumine hydrochloride
More- Cancer Res. 2025 Jan 15;85(2):231-250. [Abstract]
- J Control Release. 2026 May 4;395:114984.
- J Immunother Cancer. 2025 Nov 18;13(11):e012968. [Abstract]
- Stem Cell Res. 2021 Oct:56:102532. [Abstract]
- bioRxiv. 2026 Apr 3:2026.03.31.715681. [Abstract]
- bioRxiv. 2026 Jan 5:2026.01.05.697591. [Abstract]
- bioRxiv. 2025 Jun 19.
Customer Validation & Images
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In Vivo Imaging
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Cell Imaging/Staining
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In Vivo Efficacy Study
Biological Activity
Km: 2.06 μM (recombinant Fluc protein)[1].
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation[2]
1.1 Preparation of stock solution
Solvent: Anhydrous DMSO or ultrapure water.
Concentration: 10 mM is recommended.
Storage: Store at -20°C in dark after aliquoting and avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 1-100 μM with PBS or serum-free cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately, and should be protected from light.
2. Cell staining
2.1 Disperse cells with 100 μL PBS and suspend them in a 96-well plate at a density of 2×105.
2.2 Add AkaLumine (100 μM) and 5 mM ATP magnesium (HY-B2176) to the cells.
2.3 Analyze using a bioluminescence imaging system.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
1. Inject tumor cells into mice subcutaneously or intratibially.
2. Inject different concentrations of AkaLumine hydrochloride (0.5-33 mM; 100 μL) intraperitoneally.
3. Detect using a bioluminescence instrument 15 minutes after injection.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2558205-28-8
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Appearance Solid
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Molecular Weight 338.85
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Formula C16H19ClN2O2S
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Color Dark purple to black
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SMILES
O=C([C@@H]1N=C(/C=C/C=C/C2=CC=C(N(C)C)C=C2)SC1)O.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (7)
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Journal Impact Factor
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Most Recent
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Cancer Res
2025 Jan 15;85(2):231-250. PMID: 39495206
AkaLumine hydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Jan 15;85(2):231-250. [Abstract]
Representative brightfield (BF) and fluorescence (GFP) images of a GFP+ Foxr2-driven tumor (top, whole brain; bottom, coronal section). To enable bioluminescence imaging in vivo, a vector expressing Akaluc luciferase was codelivered. The vectors carrying Foxr2 and AkaLumine hydrochloride (15 mmol/L, 100 μL; i.p.; single dose) express green fluorescent protein (GFP) downstream of a modified 2A peptide (PQR), allowing immunochemical detection of tumor cells with GFP.
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J Immunother Cancer
JNK knockdown enhances CAR-T cell cytotoxicity through elevated NFATc1-dependent transcription in preclinical ovarian cancer models. [Abstract]2025 Nov 18;13(11):e012968. PMID: 41253493 -
Stem Cell Res
Generation of an Akaluc knock-in human embryonic stem cell reporter line using CRISPR-Cas9 technology. [Abstract]2021 Oct:56:102532. PMID: 34509917
AkaLumine hydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res. 2021 Oct:56:102532. [Abstract]
Akaluc activity was detected in vitro using bioluminescence imaging (BLI). Cells were dispersed and suspended by 100 μL PBS in a 96-well plate at a density of 2 × 105. Added 100 µM AkaLumine hydrochloride and 5 mM ATP-mg, used the BLI to analyze.
AkaLumine hydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res. 2021 Oct:56:102532. [Abstract]
Akaluc activity was detected in vivo using bioluminescence imaging (BLI). 1 million cells were transplanted into femoral ditch of the SCID mice. After 1–2 month, we injected 30 mM, 100 μL AkaLumine hydrochloride for 15 min. Then, gas anaesthetized mice and used the IVIS Spectrum to analyze.
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bioRxiv
Pharmacologic DPP-4 inhibition promotes CD8+ T cell metabolic fitness to enhance anti-tumor activity. [Abstract]2026 Apr 3:2026.03.31.715681. PMID: 41959339 -
bioRxiv
Atomic Layering Thermostable Antigen and Adjuvant (ALTA®) platform provides unique antigen delivery system through controlled release to improve immune response to vaccination. [Abstract]2026 Jan 5:2026.01.05.697591. PMID: 41542521 -
Solvent & Solubility
H2O : 50 mg/mL (147.56 mM; Need ultrasonic)
DMSO : 50 mg/mL (147.56 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Kuchimaru T, et al. A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat Commun. 2016 Jun 14;7:11856. [Content Brief]
[2]. Zhou M, et al. Generation of an Akaluc knock-in human embryonic stem cell reporter line using CRISPR-Cas9 technology. Stem Cell Res. 2021 Oct;56:102532. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.9512 mL | 14.7558 mL | 29.5116 mL | 73.7790 mL |
| 5 mM | 0.5902 mL | 2.9512 mL | 5.9023 mL | 14.7558 mL | |
| 10 mM | 0.2951 mL | 1.4756 mL | 2.9512 mL | 7.3779 mL | |
| 15 mM | 0.1967 mL | 0.9837 mL | 1.9674 mL | 4.9186 mL | |
| 20 mM | 0.1476 mL | 0.7378 mL | 1.4756 mL | 3.6889 mL | |
| 25 mM | 0.1180 mL | 0.5902 mL | 1.1805 mL | 2.9512 mL | |
| 30 mM | 0.0984 mL | 0.4919 mL | 0.9837 mL | 2.4593 mL | |
| 40 mM | 0.0738 mL | 0.3689 mL | 0.7378 mL | 1.8445 mL | |
| 50 mM | 0.0590 mL | 0.2951 mL | 0.5902 mL | 1.4756 mL | |
| 60 mM | 0.0492 mL | 0.2459 mL | 0.4919 mL | 1.2296 mL | |
| 80 mM | 0.0369 mL | 0.1844 mL | 0.3689 mL | 0.9222 mL | |
| 100 mM | 0.0295 mL | 0.1476 mL | 0.2951 mL | 0.7378 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
AkaLumine hydrochloride Related Classifications
HY-112641A Related Classifications
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