AkaLumine hydrochloride

Name: AkaLumine hydrochloride
Brand: MedChemExpress
SKU: HY-112641A
Rating: 5.0 (5 reviews)

Cat. No.: HY-112641A Purity: 99.92%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

AkaLumine hydrochloride is a D-luciferin (HY-12591A) analogue with a K_m of 2.06 μM for recombinant firefly luciferase (Fluc) protein. AkaLumine hydrochloride emits near-infrared (NIR) light (λmax=677 nm) in reactions with native Fluc. AkaLumine hydrochloride has high tissue-penetration and increases detection sensitivity from deep-tissue targets.

For research use only. We do not sell to patients.

CAS No. : 2558205-28-8

Size	Stock	Quantity
Free Sample (0.1 - 0.2 mg)	Apply Now
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of AkaLumine hydrochloride:

AkaLumine Get quote

5 Publications Citing Use of MCE AkaLumine hydrochloride

In Vivo Imaging

Cell Imaging/Staining

In Vivo Efficacy Study

: AkaLumine hydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Jan 15;85(2):231-250. [Abstract]; Representative brightfield (BF) and fluorescence (GFP) images of a GFP⁺ Foxr2-driven tumor (top, whole brain; bottom, coronal section). To enable bioluminescence imaging in vivo, a vector expressing Akaluc luciferase was codelivered. The vectors carrying Foxr2 and AkaLumine hydrochloride (15 mmol/L, 100 μL; i.p.; single dose) express green fluorescent protein (GFP) downstream of a modified 2A peptide (PQR), allowing immunochemical detection of tumor cells with GFP.

: AkaLumine hydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res. 2021 Oct:56:102532. [Abstract]; Akaluc activity was detected in vitro using bioluminescence imaging (BLI). Cells were dispersed and suspended by 100 μL PBS in a 96-well plate at a density of 2 × 10⁵. Added 100 µM AkaLumine hydrochloride and 5 mM ATP-mg, used the BLI to analyze.

: AkaLumine hydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res. 2021 Oct:56:102532. [Abstract]; Akaluc activity was detected in vivo using bioluminescence imaging (BLI). 1 million cells were transplanted into femoral ditch of the SCID mice. After 1–2 month, we injected 30 mM, 100 μL AkaLumine hydrochloride for 15 min. Then, gas anaesthetized mice and used the IVIS Spectrum to analyze.

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

Km: 2.06 μM (recombinant Fluc protein)^[1].

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation^[2]
1.1 Preparation of stock solution
Solvent: Anhydrous DMSO or ultrapure water.
Concentration: 10 mM is recommended.
Storage: Store at -20°C in dark after aliquoting and avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 1-100 μM with PBS or serum-free cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately, and should be protected from light.

2. Cell staining
2.1 Disperse cells with 100 μL PBS and suspend them in a 96-well plate at a density of 2×10⁵.
2.2 Add AkaLumine (100 μM) and 5 mM ATP magnesium (HY-B2176) to the cells.
2.3 Analyze using a bioluminescence imaging system.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Subcutaneous/orthotopic tumor mouse imaging^[1]
1. Inject tumor cells into mice subcutaneously or intratibially.
2. Inject different concentrations of AkaLumine hydrochloride (0.5-33 mM; 100 μL) intraperitoneally.
3. Detect using a bioluminescence instrument 15 minutes after injection.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

338.85

Formula

C₁₆H₁₉ClN₂O₂S

CAS No.

2558205-28-8

Appearance

Solid

Color

Dark purple to black

SMILES

O=C([C@@H]1N=C(/C=C/C=C/C2=CC=C(N(C)C)C=C2)SC1)O.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (147.56 mM; Need ultrasonic)

DMSO : 50 mg/mL (147.56 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9512 mL	14.7558 mL	29.5116 mL
5 mM	0.5902 mL	2.9512 mL	5.9023 mL
10 mM	0.2951 mL	1.4756 mL	2.9512 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (246 KB) HNMR (385 KB) RP-HPLC (265 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

Dyeing Example

Description: AkaLumine hydrochloride can be used to detect Akaluc expression in vitro.
Method: For cell labeling.
1. Disperse and suspend cells by 100 μL PBS in a 96-well plate at a density of 2×10⁵.
2. Add AkaLumine hydrochloride (100 µM) and 5 mM ATP-mg to cells, and use a bioluminescence imaging system to analyze.

References

[1]. Kuchimaru T, et al. A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat Commun. 2016 Jun 14;7:11856. [Content Brief]

[2]. Zhou M, et al. Generation of an Akaluc knock-in human embryonic stem cell reporter line using CRISPR-Cas9 technology. Stem Cell Res. 2021 Oct;56:102532. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.9512 mL	14.7558 mL	29.5116 mL	73.7790 mL
	5 mM	0.5902 mL	2.9512 mL	5.9023 mL	14.7558 mL
	10 mM	0.2951 mL	1.4756 mL	2.9512 mL	7.3779 mL
	15 mM	0.1967 mL	0.9837 mL	1.9674 mL	4.9186 mL
	20 mM	0.1476 mL	0.7378 mL	1.4756 mL	3.6889 mL
	25 mM	0.1180 mL	0.5902 mL	1.1805 mL	2.9512 mL
	30 mM	0.0984 mL	0.4919 mL	0.9837 mL	2.4593 mL
	40 mM	0.0738 mL	0.3689 mL	0.7378 mL	1.8445 mL
	50 mM	0.0590 mL	0.2951 mL	0.5902 mL	1.4756 mL
	60 mM	0.0492 mL	0.2459 mL	0.4919 mL	1.2296 mL
	80 mM	0.0369 mL	0.1844 mL	0.3689 mL	0.9222 mL
	100 mM	0.0295 mL	0.1476 mL	0.2951 mL	0.7378 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.