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  2. TokeOni

TokeOni (Synonyms: AkaLumine hydrochloride)

Cat. No.: HY-112641A Purity: 96.31%
Handling Instructions

TokeOni (AkaLumine hydrochloride) is a luciferin analogue, with a Km of 2.06 μM for recombinant Fluc protein.

For research use only. We do not sell to patients.

TokeOni Chemical Structure

TokeOni Chemical Structure

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 116 In-stock
Estimated Time of Arrival: December 31
5 mg USD 105 In-stock
Estimated Time of Arrival: December 31
10 mg USD 175 In-stock
Estimated Time of Arrival: December 31
50 mg USD 700 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1200 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

TokeOni (AkaLumine hydrochloride) is a luciferin analogue, with a Km of 2.06 μM for recombinant Fluc protein.

IC50 & Target

Km: 2.06 μM (recombinant Fluc protein)[1].

In Vitro

Interestingly, in both LLC/luc and MDA-MB-231/luc cells treated with TokeOni (AkaLumine hydrochloride), the signal is maximal at lower concentrations (by 2.5 μM), whereas the bioluminescence generated by D-luciferin and CycLuc1 increase as concentrations increase further, not appearing to reach a maximal signal even at 250 μM[1].

In Vivo

TokeOni (AkaLumine hydrochloride) displays greatly enhanced signals emanating from metastasis in the lung, as demonstrated by 8.1-fold higher with TokeOni as compared with D-luciferin administration. To evaluate the advantage of TokeOni (AkaLumine hydrochloride) over CycLuc1 in detecting targets in deep tissue, bioluminescence images are compared between TokeOni- and CycLuc1-treated mice 15 min after intravenous injection of LLC/luc cells at the dose of 5 mM, which is the maximum concentration of CycLuc1 due to its low water solubility. TokeOni exhibits 3.3-fold increase in detection sensitivity of disseminated cancer cells in the lung compared with CycLuc1. The advantage of TokeOni is further confirmed by imaging the same mice bearing lung metastasis after intraperitoneal injection of 5 mM substrates in order of CycLuc1 and AkaLumine hydrochloride, and in the inverse order at an 8-h interval. TokeOni displays about fourfold increase in signals from lung metastasis compared with CycLuc1[1].

Molecular Weight

338.85

Formula

C₁₆H₁₉ClN₂O₂S

SMILES

O=C([[email protected]@H]1N=C(/C=C/C=C/C2=CC=C(N(C)C)C=C2)SC1)O.Cl

Shipping

Room temperature in continental US; may vary elsewhere

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (147.56 mM; Need ultrasonic)

DMSO : 25 mg/mL (73.78 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9512 mL 14.7558 mL 29.5116 mL
5 mM 0.5902 mL 2.9512 mL 5.9023 mL
10 mM 0.2951 mL 1.4756 mL 2.9512 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (7.38 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (7.38 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (7.38 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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TokeOni
Cat. No.:
HY-112641A
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