1. Dye Reagents

TokeOni (Synonyms: AkaLumine hydrochloride)

Cat. No.: HY-112641A
Handling Instructions

AkaLumine hydrochloride is a luciferin analogue, with a Km of 2.06 μM for recombinant Fluc protein.

For research use only. We do not sell to patients.

TokeOni Chemical Structure

TokeOni Chemical Structure

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Customer Review

  • Biological Activity

  • Technical Information

  • Purity & Documentation

  • References

Description

AkaLumine hydrochloride is a luciferin analogue, with a Km of 2.06 μM for recombinant Fluc protein.

IC50 & Target

Km: 2.06 μM (recombinant Fluc protein)[1].

In Vitro

Interestingly, in both LLC/luc and MDA-MB-231/luc cells treated with AkaLumine hydrochloride (AkaLumine-HCl), the signal is maximal at lower concentrations (by 2.5 μM), whereas the bioluminescence generated by D-luciferin and CycLuc1 increase as concentrations increase further, not appearing to reach a maximal signal even at 250 μM[1].

In Vivo

AkaLumine hydrochloride (AkaLumine-HCl) displays greatly enhanced signals emanating from metastasis in the lung, as demonstrated by 8.1-fold higher with AkaLumine hydrochloride as compared with D-luciferin administration. To evaluate the advantage of AkaLumine hydrochloride over CycLuc1 in detecting targets in deep tissue, bioluminescence images are compared between AkaLumine hydrochloride- and CycLuc1-treated mice 15 min after intravenous injection of LLC/luc cells at the dose of 5 mM, which is the maximum concentration of CycLuc1 due to its low water solubility. AkaLumine hydrochloride exhibits 3.3-fold increase in detection sensitivity of disseminated cancer cells in the lung compared with CycLuc1. The advantage of AkaLumine hydrochloride is further confirmed by imaging the same mice bearing lung metastasis after intraperitoneal injection of 5 mM substrates in order of CycLuc1 and AkaLumine hydrochloride, and in the inverse order at an 8-h interval. AkaLumine hydrochloride displays about fourfold increase in signals from lung metastasis compared with CycLuc1[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9512 mL 14.7558 mL 29.5116 mL
5 mM 0.5902 mL 2.9512 mL 5.9023 mL
10 mM 0.2951 mL 1.4756 mL 2.9512 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Molecular Weight

338.85

Formula

C₁₆H₁₉ClN₂O₂S

SMILES

O=C([[email protected]@H]1N=C(/C=C/C=C/C2=CC=C(N(C)C)C=C2)SC1)O.Cl

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
TokeOni
Cat. No.:
HY-112641A
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TokeOni

Cat. No.: HY-112641A