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  4. AkaLumine

AkaLumine is a D-luciferin (HY-12591A) analogue with a Km of 2.06 μM for recombinant firefly luciferase (Fluc) protein. AkaLumine emits near-infrared (NIR) light (λmax=677 nm) in reactions with native Fluc. AkaLumine has high tissue-penetration and increases detection sensitivity from deep-tissue targets.

For research use only. We do not sell to patients.

CAS No. : 1176235-08-7

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AkaLumine Related Antibodies

Top Publications Citing Use of Products

    AkaLumine purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Jan 15;85(2):231-250.  [Abstract]

    Representative brightfield (BF) and fluorescence (GFP) images of a GFP+ Foxr2-driven tumor (top, whole brain; bottom, coronal section). To enable bioluminescence imaging in vivo, a vector expressing Akaluc luciferase was codelivered. The vectors carrying Foxr2 and AkaLumine hydrochloride (15 mmol/L, 100 μL; i.p.; single dose) express green fluorescent protein (GFP) downstream of a modified 2A peptide (PQR), allowing immunochemical detection of tumor cells with GFP.

    AkaLumine purchased from MedChemExpress. Usage Cited in: Stem Cell Res. 2021 Oct:56:102532.  [Abstract]

    Akaluc activity was detected in vitro using bioluminescence imaging (BLI). Cells were dispersed and suspended by 100 μL PBS in a 96-well plate at a density of 2 × 105. Added 100 µM AkaLumine hydrochloride and 5 mM ATP-mg, used the BLI to analyze.

    AkaLumine purchased from MedChemExpress. Usage Cited in: Stem Cell Res. 2021 Oct:56:102532.  [Abstract]

    Akaluc activity was detected in vivo using bioluminescence imaging (BLI). 1 million cells were transplanted into femoral ditch of the SCID mice. After 1–2 month, we injected 30 mM, 100 μL AkaLumine hydrochloride for 15 min. Then, gas anaesthetized mice and used the IVIS Spectrum to analyze.

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    Description

    AkaLumine is a D-luciferin (HY-12591A) analogue with a Km of 2.06 μM for recombinant firefly luciferase (Fluc) protein. AkaLumine emits near-infrared (NIR) light (λmax=677 nm) in reactions with native Fluc. AkaLumine has high tissue-penetration and increases detection sensitivity from deep-tissue targets[1].

    IC50 & Target

    Km: 2.06 μM[1].
    In Vitro

    Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
    1. Solution preparation[2]
    1.1 Preparation of stock solution
    Solvent: Anhydrous DMSO or ultrapure water.
    Concentration: 10 mM is recommended.
    Storage: Store at -20°C in dark after aliquoting and avoid repeated freezing and thawing.
    1.2 Preparation of working solution
    Dilute to 1-100 μM with PBS or serum-free cell culture medium (optimized according to the experiment).
    Note: The working solution should be prepared and used immediately, and should be protected from light.

    2. Cell staining
    2.1 Disperse cells with 100 μL PBS and suspend them in a 96-well plate at a density of 2×105.
    2.2 Add AkaLumine hydrochloride (100 μM) and 5 mM ATP magnesium (HY-B2176) to the cells.
    2.3 Analyze using a bioluminescence imaging system.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    AkaLumine Related Antibodies
    In Vivo

    Subcutaneous/orthotopic tumor mouse imaging[1]
    1. Inject tumor cells into mice subcutaneously or intratibially.
    2. Inject different concentrations of AkaLumine hydrochloride (0.5-33 mM; 100 μL) intraperitoneally.
    3. Detect using a bioluminescence instrument 15 minutes after injection.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    302.39

    Formula

    C16H18N2O2S
    CAS No.
    SMILES

    O=C([C@@H]1N=C(/C=C/C=C/C2=CC=C(N(C)C)C=C2)SC1)O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
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    AkaLumine Related Classifications

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    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    AkaLumine1176235-08-7Fluorescent DyeBioluminescenceNIRDeep tissue imagingLLC/luc tumors.Inhibitorinhibitorinhibit

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