1. Cell Cycle/DNA Damage
  2. DNA Alkylator/Crosslinker
  3. Chlorambucil

Chlorambucil (Synonyms: CB-1348; WR-139013)

Cat. No.: HY-13593 Purity: 98.79%
Handling Instructions

Chlorambucil is an alkylating agent with antitumor activity.

For research use only. We do not sell to patients.

Chlorambucil Chemical Structure

Chlorambucil Chemical Structure

CAS No. : 305-03-3

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10 mM * 1 mL in DMSO USD 158 In-stock
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Based on 1 publication(s) in Google Scholar

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Chlorambucil is an alkylating agent with antitumor activity.

IC50 & Target

DNA Alkylator[1]

In Vitro

Chlorambucil can deprive the function of complementary strands of DNA molecules via alkalization-induced cross interaction, and then inhibits tumor cell proliferation. Chlorambucil (0, 2.5, 5, 10 μM) exhibits slight inhibitory effect on Raji cell apoptosis, but potently increases DR4 and DR5 mRNA expression in Raji cells. Chlorambucil (10 μM) in combination with Tumor necrosis factor (TNF) related apoptosis inducing ligand (TRAIL, 80 ng/ml) has synergistic effect on Raji cell apoptosis and inhibition on proliferation[1].

In Vivo

Chlorambucil (0.2 mg/kg, p.o.) in combination with levamisole (5 mg/kg) has enhanced anti-cancer effect on Ehrlich ascites carcinoma which elevates apoptosis of Ehrlich ascites carcinoma and the survival rate of the mice. However, Chlorambucil exhibits adverse effects on the liver and kidneys of mice[2].

Clinical Trial
Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 24 mg/mL (78.89 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2872 mL 16.4360 mL 32.8720 mL
5 mM 0.6574 mL 3.2872 mL 6.5744 mL
10 mM 0.3287 mL 1.6436 mL 3.2872 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

Cultured cells at log-growth phase are digested by trypsin into single cell suspension and are seeded into 96-well plate at 1000 per well density. The plate is placed in a 37°C chamber with 5% CO2. After attached growth for 24 h, cells are treated with TRAIL at 0, 20, 40 and 80 ng/mL or Chlorambucil at 0, 2.5, 5 and 10 μM for 48 h. 10 μL CCK-8 reagent is added to each well, followed by incubation at 37°C for 4 h. Absorbance values at 450 nm are then measured by a micro-plate reader. Six parallel samples are performed in each treatment group. Cell proliferation rate (%) = mean value of experimental group/mean value of control group × 100%[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Mice[2]
Female Swiss mice are divided randomly into five group (20 mice per group). Group 1 is kept as the control group, Group 2 receives intraperitoneal injection of by 2.5 × 106 Ehrlich ascites carcinoma cell, Gropu 3 is treated orally with Chlorambucil 0.2 mg/kg body weight, Group 4 is treated orally with levamisole (5 mg/kg body weight) and Group 5 is treated orally with a combination of Chlorambucil and levamisole each day, using a bent stainless steel stomach tube[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

304.21

Formula

C₁₄H₁₉Cl₂NO₂

CAS No.

305-03-3

SMILES

O=C(O)CCCC1=CC=C(N(CCCl)CCCl)C=C1

Shipping

Room temperature in continental US; may vary elsewhere

Purity: 98.79%

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Chlorambucil

Cat. No.: HY-13593