2. Neuronal Signaling Stem Cell/Wnt
  3. γ-secretase
  4. Compound E

Compound E  (Synonyms: γ-Secretase-IN-1)

Cat. No.: HY-14176 Purity: 99.97% ee.: 100.00%
COA
SDS
Handling Instructions Technical Support

Compound E is a γ-secretase inhibitor. Compound E blocks β-amyloid(40), β-amyloid(42), and Notch γ-secretase cleavage with IC50s of 0.24, 0.37, 0.32 nM, respectively.

For research use only. We do not sell to patients.

CAS No. : 209986-17-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
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500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 17 publication(s) in Google Scholar

Other Forms of Compound E:

Compound E Related Antibodies

Top Publications Citing Use of Products

    Compound E purchased from MedChemExpress. Usage Cited in: Fundam Res. 2026 Jan 10.

    Schematic representation of structural morphogenesis of human post-implantation embryoids from hESCs in 3D culture. H9 human embryonic stem cell (hESC) clusters embedded in 100% Matrigel to promote cell adhesion tension. The embedded hESC clusters were then supplemented with CHIR99021, FGF2, LDN193189 and Compound E (2 μM; 3 d) in an N2/B27 basal medium to induce post-implantation embryoid formation.

    Compound E purchased from MedChemExpress. Usage Cited in: Mol Neurodegener. 2025 Aug 28;20(1):94.  [Abstract]

    ISF Aβ1-x obtained by microdialysis from 1-month-old APP/PS1:Ms4a6d +/+ mice (blue) and APP/PS1:Ms4a6d +/− mice (magenta). Baseline Aβ1-x levels were defined as the mean concentration from hours 3–5 after probe insertion. Aβ1-x values after γ-secretase inhibitor Compound E (6.7 mg/kg; i.p.) treatment at each time point were expressed as percentage of baseline mean (% baseline). Raw data were fitted with linear trend lines.

    Compound E purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Aug 19.

    Efects of protease inhibitors on extracellular and intracellular HO-1 protein levels in cultured rat astrocytes. Astrocytes were incubated with NH4Cl (5 mM) for 48 h before cysteine protease inhibitors (Compound E, 5 µM; E64d, 50 µM) or the ER-associated degradation (ERAD) inhibitor eeyarestatin 1 (ESI, 20 µM) were added and cells were incubated for another 24 h. Fulllength and truncated HO-1 protein were analyzed in cell culture supernatants and cellular lysates by Western blot and quantified by densitometry.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Compound E is a γ-secretase inhibitor. Compound E blocks β-amyloid(40), β-amyloid(42), and Notch γ-secretase cleavage with IC50s of 0.24, 0.37, 0.32 nM, respectively.

    IC50 & Target

    IC50: 0.24 nM (β-amyloid(40)), 0.37 nM (β-amyloid(42)), 0.24 nM (Notch)[1]
    Cellular Effect
    Cell Line Type Value Description References
    SH-SY5Y IC50
    1 nM
    Compound: 66
    Displacement of [3H]5-chloro-N-((2S,3R)-5,5,5-trifluoro-1-hydroxy-3-methylpentan-2-yl)thiophene-2-sulfonamide from gamma secretase in human SH-SY5Y cells by competitive binding assay
    Displacement of [3H]5-chloro-N-((2S,3R)-5,5,5-trifluoro-1-hydroxy-3-methylpentan-2-yl)thiophene-2-sulfonamide from gamma secretase in human SH-SY5Y cells by competitive binding assay
    		[PMID: 19694467]
    SH-SY5Y IC50
    2.7 nM
    Compound: 66
    Inhibition of gamma secretase-mediated amyloid beta (1 to 40) production in human SH-SY5Y cells
    Inhibition of gamma secretase-mediated amyloid beta (1 to 40) production in human SH-SY5Y cells
    		[PMID: 19694467]
    In Vitro

    Compound E reduces the proliferation of T47D, and MCF-7 cell lines by less than 50% at a concentration of 50 μM[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Compound E Related Antibodies
    Clinical Trial
    Molecular Weight

    490.50

    Formula

    C27H24F2N4O3
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    FC1=CC(CC(N[C@@H](C)C(N[C@@H]2C(N(C)C(C=CC=C3)=C3C(C4=CC=CC=C4)=N2)=O)=O)=O)=CC(F)=C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (203.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0387 mL 10.1937 mL 20.3874 mL
    5 mM 0.4077 mL 2.0387 mL 4.0775 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.88 mg/mL (5.87 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.88 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (28.8 mg/mL) to 900 μL Corn oil, and mix evenly.

    • Protocol 2

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (5.10 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.97% ee.: 100.00%

    SDS (393 KB)

    COA (254 KB) HNMR (388 KB) RP-HPLC (245 KB) NP-HPLC (315 KB) MS (69 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    The breast cancer cell lines MDA-MB-231, T47D, and MCF-7 are treated with Compound E at concentrations in the range 0.01-50 μM for 48 h and their viability is determined using a Coulter counter[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.0387 mL 10.1937 mL 20.3874 mL 50.9684 mL
    5 mM 0.4077 mL 2.0387 mL 4.0775 mL 10.1937 mL
    10 mM 0.2039 mL 1.0194 mL 2.0387 mL 5.0968 mL
    15 mM 0.1359 mL 0.6796 mL 1.3592 mL 3.3979 mL
    20 mM 0.1019 mL 0.5097 mL 1.0194 mL 2.5484 mL
    25 mM 0.0815 mL 0.4077 mL 0.8155 mL 2.0387 mL
    30 mM 0.0680 mL 0.3398 mL 0.6796 mL 1.6989 mL
    40 mM 0.0510 mL 0.2548 mL 0.5097 mL 1.2742 mL
    50 mM 0.0408 mL 0.2039 mL 0.4077 mL 1.0194 mL
    60 mM 0.0340 mL 0.1699 mL 0.3398 mL 0.8495 mL
    80 mM 0.0255 mL 0.1274 mL 0.2548 mL 0.6371 mL
    100 mM 0.0204 mL 0.1019 mL 0.2039 mL 0.5097 mL
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    Compound E Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Compound E209986-17-4γ-Secretase-IN-1γ-secretaseGamma secretaseInhibitorinhibitorinhibit

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