2. Others Cell Cycle/DNA Damage
  3. Biochemical Assay Reagents Disulfidptosis
  4. DL-Dithiothreitol

DL-dithiothreitol  (Synonyms: DTT; DL-ジチオトレイトール)

製品番号: HY-15917 純度: 98.31%
COA 取扱説明書 Technical Support

DL-Dithiothreitol (DTT) is a strong reductant with anti-disulfidptosis activity. When DL-Dithiothreitol is oxidized, it forms a stable six-membered ring with an internal disulfide bond.

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CAS 番号 : 3483-12-3

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 60 在庫あり
Solution
10 mM * 1 mL in DMSO USD 60 在庫あり
Solid
500 mg $55 在庫あり
1 g $66 在庫あり
5 g $112 在庫あり
10 g   お問い合わせ  
50 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 42 publication(s) in Google Scholar

Other Forms of DL-Dithiothreitol:

DL-dithiothreitol 関連抗体

Top Publications Citing Use of Products

顧客検証

Cell Imaging/Staining
WB
Flow Cytometry

    DL-Dithiothreitol purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Oct;48(5):3146-3155.  [Abstract]

    The melanocytes were treated with TM (3 μM), thapsigargin (1 μM), or DTT (1 mM) for 24 h to induce ER stress. Increased expression of PERK, IRE1α, and CHOP.

    DL-Dithiothreitol purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Oct;48(5):3146-3155.  [Abstract]

    The melanocytes were treated with TM (3 μM), thapsigargin (1 μM), or DTT (1 mM) for 24 h to induce ER stress.

    DL-Dithiothreitol purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Oct;48(5):3146-3155.  [Abstract]

    The melanocytes were treated with TM (3 μM), thapsigargin (1 μM), or DTT (1 mM) for 24 h to induce ER stress. ER stress induced an increase in the apoptosis of melanocytes.

    DL-Dithiothreitol purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Oct;48(5):3146-3155.  [Abstract]

    Decreased Sirt1 protein levels in melanocytes after 24 h treatment with TM (3 μM), TG (1 μM), and DTT(1 mM).

    DL-Dithiothreitol purchased from MedChemExpress. Usage Cited in: Vet Microbiol. 2020 Aug:247:108786.  [Abstract]

    Vero cells were infected with PRV HB1201(0.1 MOI) for 12 h, followed by treatment with 0.5 mM AS for 30 min or 2 mM DTT for 1 h, respectively.

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    DL-Dithiothreitol (DTT) is a strong reductant with anti-disulfidptosis activity. When DL-Dithiothreitol is oxidized, it forms a stable six-membered ring with an internal disulfide bond[1][2].

    体外実験

    DL-Dithiothreitol is widely used in biochemistry works to reduce dissulfide bridges, protect biomolecules, in sample preparation, and to denature proteins before electrophoresis analysis (SDS-PAGE).
    DL-Dithiothreitol (0.5 mM) prevents dithiothreitol stress and inhibits dithiothreitol cell death in DLD-1 and HCT116 cells under glucose starvation[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DL-dithiothreitol 関連抗体
    分子量

    154.25

    分子式

    C4H10O2S2
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    SC[C@@H](O)[C@H](O)CS
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (648.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.4830 mL 32.4149 mL 64.8298 mL
    5 mM 1.2966 mL 6.4830 mL 12.9660 mL
    10 mM 0.6483 mL 3.2415 mL 6.4830 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (16.21 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (16.21 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (648.30 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.8%

    COA (245 KB) HNMR (112 KB) GC (178 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 6.4830 mL 32.4149 mL 64.8298 mL 162.0746 mL
    5 mM 1.2966 mL 6.4830 mL 12.9660 mL 32.4149 mL
    10 mM 0.6483 mL 3.2415 mL 6.4830 mL 16.2075 mL
    15 mM 0.4322 mL 2.1610 mL 4.3220 mL 10.8050 mL
    20 mM 0.3241 mL 1.6207 mL 3.2415 mL 8.1037 mL
    25 mM 0.2593 mL 1.2966 mL 2.5932 mL 6.4830 mL
    30 mM 0.2161 mL 1.0805 mL 2.1610 mL 5.4025 mL
    40 mM 0.1621 mL 0.8104 mL 1.6207 mL 4.0519 mL
    50 mM 0.1297 mL 0.6483 mL 1.2966 mL 3.2415 mL
    60 mM 0.1080 mL 0.5402 mL 1.0805 mL 2.7012 mL
    80 mM 0.0810 mL 0.4052 mL 0.8104 mL 2.0259 mL
    100 mM 0.0648 mL 0.3241 mL 0.6483 mL 1.6207 mL
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    DL-Dithiothreitol Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DL-Dithiothreitol3483-12-3DTT rel-(2R,3R)-1,4-Dimercapto-2,3-butanediolBiochemical Assay ReagentsDisulfidptosisInhibitorinhibitorinhibit

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    製品名:
    DL-Dithiothreitol
    製品番号:
    HY-15917
    数量:
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