DiD perchlorate

Name: DiD perchlorate
Brand: MedChemExpress
SKU: HY-D1028
Rating: 5.0 (36 reviews)

Cat. No.: HY-D1028 Purity: 98.64%: Data Sheet
COA

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Handling Instructions
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Technical Support

DiD is a long-chain carbocyanine dye. Carbocyanine dyes are widely used as Di to label cells, organelles, liposomes, viruses and lipoproteins (Ex/Em = 633/665 nm).

For research use only. We do not sell to patients.

CAS No. : 127274-91-3

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 36 publication(s) in Google Scholar

Other Forms of DiD perchlorate:

36 Publications Citing Use of MCE DiD perchlorate

Cell Imaging/Staining

In Vivo Imaging

: DiD perchlorate purchased from MedChemExpress. Usage Cited in: Nature. 2025 Nov;647(8089):506-516. [Abstract]; Primary macrophages were isolated as described above. After staining 4T1 cells with DID (MCE, HY-D1028) and macrophages with CFSE, cells were cultured with an effector-to-target ratio of 3:1 at 37 °C for 24 h. The phagocytosis effect was visualized by confocal imaging. 4T1 cells and macrophages were visualized by DiD dye (magenta) and CFSE dye (green).

: DiD perchlorate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10096. [Abstract]; Fluorescence images of cellular uptake in Huh7, THLE3, PANC1, M2, M1, and CM after Sv@PM-M2p treatment (Scale bar = 100 μm), along with the quantification of red fluorescence intensity (n = 3 independent experiments). Sv@PM-M2p was labeled with DiD (red), the nuclei and cytoskeletons were stained with DAPI (blue) and Phalloidin (green), respectively.

: DiD perchlorate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10096. [Abstract]; Fluorescence images of cellular uptake in the Huh7 and M2 co-culture system after Sv@PM-M2p treatment (Scale bar = 100 μm), along with the quantification of red fluorescence intensity (n = 3 independent experiments). Sv@PM-M2p was labeled with DiD (red), the nuclei and cytoskeletons were stained with DAPI (blue) and Phalloidin (green), respectively.

: DiD perchlorate purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Oct 14;42(10):1729-1746.e8.; FITC-labeled butyrate translocated to the nucleus of CD8⁺ T cells after 6 h of co-culture, as determined by confocal microscopy. CD8⁺ T cells were stained using FITC-labeled butyrate (green), DiD perchlorate (red), and DAPI (blue).

: DiD perchlorate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Aug 9:e2402329. [Abstract]; Fluorescence imaging was performed on subcutaneous tumor-bearing mice after being injected with DiD-labeled LNPs through the tail vein.

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Biological Activity
Protocol
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References
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Description

DiD is a long-chain carbocyanine dye. Carbocyanine dyes are widely used as Di to label cells, organelles, liposomes, viruses and lipoproteins (Ex/Em = 633/665 nm)^[2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparing Stain Solutions of DiD perchlorate
1.1 Prepare DMF, DMSO or ethanol stock solutions:
The stock solutions should be prepared in dimethyl formamide (DMF), dimethylsulfoxide (DMSO, or ethanol DMSO at 1-5 mM.
1.2 Prepare working solutions:
Dilute the stock solutions into a suitable buffer such as serum-free culture medium, HBSS or PBS to make 1-5 μM working solutions. We do not recommend storing the aqueous solution for more than one day.
Note: The final concentration of the working solution should be empirically determined for different cell types and/or experimental conditions.
2. Cell staining (Suspension cells)
2.1 Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×10⁶/mL.
2.2 Add 1 mL of DiD perchlorate working solution, and then incubate at room temperature for 5-30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
3. Cell staining (Adherent cells)
3.1 Culture adherent cells on sterile coverslips.
3.2 Remove the coverslip from the medium and aspirate excess medium.
3.3 Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 minutes.
3.4 Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Two weeks after injection of stained cells, a single, bright, DiD perchlorate (DiD)-positive cells located between 15 to 40 μm from the endosteum is found. Results reveal a progressive appearance of cell clusters of decreased dye intensity, consistent with the partitioning of DiD perchlorate label on cell division^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

959.90

Formula

C₆₁H₉₉ClN₂O₄

CAS No.

127274-91-3

Appearance

Solid

Color

Blue to dark blue

Emission (Em)

665

Excitation (Ex)

633

SMILES

CCCCCCCCCCCCCCCCCCN1C2=C(C=CC=C2)C(C)(C)/C1=C\C=C\C=C\C3=[N+](C4=C(C=CC=C4)C3(C)C)CCCCCCCCCCCCCCCCCC.O=Cl(=O)([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (26.04 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.0418 mL	5.2089 mL	10.4178 mL
5 mM	0.2084 mL	1.0418 mL	2.0836 mL
10 mM	0.1042 mL	0.5209 mL	1.0418 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.96%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (239 KB) HNMR (321 KB) LCMS (108 KB)

Handling Instructions (2659 KB)

Dyeing Example

Description: DiD perchlorate is a far-red fluorescent lipophilic cyanine dye, it can be used in intravital imaging.
Method: For extracellular vesicle (EVs) staining.
1. Purified extracellular vesicles suspended in PBS and incubate with DiD perchlorate (1 μM; 15 min), and by an additional wash to remove the free dye.
2. DiD perchlorate-labeled EVs are resuspended in PBS and injected intravenously into 6-week-old female hACE2 mice (C57BL/6) with 150 μg EV per mice.
3. The hACE2 mice are sacrificed at 24 h, 48 h,72 h and 96 h post injection.
4. The red fluorescence of the whole body is acquired by IVIS spectrum (Caliper Life Sciences).

References

[1]. Gan WB, et al. Multicolor "DiOlistic" labeling of the nervous system using lipophilic dye combinations. Neuron. 2000 Aug;27(2):219-25. [Content Brief]

[2]. Kenji Yumoto, et al. A novel method for monitoring tumor dormancy using fluorescent dye DiD. Cytometry A. 2014 Jun; 85(6): 548–555. [Content Brief]

[3]. Meng Li, et al. In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye. J Vis Exp. 2017; (128): 56273. [Content Brief]

[4]. Lo Celso C, et al. Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche. [Content Brief]

Animal Administration
^[1]

1 to 5×10⁵ cells per mL HSPCs are stained with 5 μM DiD perchlorate (DiD) or 7.5 μM DiI in PBS without serum for 10 min at 37°C, washed once in PBS and immediately injected into the tail vein of recipient mice. Unless stated differently, each imaged CD45.2 mouse receive 8,000 to 15,000 labelled CD45.1 HSPCs together with 3 to 5×10⁵ CD45.2 supportive whole bone-marrow mononuclear cells to ensure survival^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Gan WB, et al. Multicolor "DiOlistic" labeling of the nervous system using lipophilic dye combinations. Neuron. 2000 Aug;27(2):219-25. [Content Brief]

[2]. Kenji Yumoto, et al. A novel method for monitoring tumor dormancy using fluorescent dye DiD. Cytometry A. 2014 Jun; 85(6): 548–555. [Content Brief]

[3]. Meng Li, et al. In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye. J Vis Exp. 2017; (128): 56273. [Content Brief]

[4]. Lo Celso C, et al. Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.0418 mL	5.2089 mL	10.4178 mL	26.0444 mL
	5 mM	0.2084 mL	1.0418 mL	2.0836 mL	5.2089 mL
	10 mM	0.1042 mL	0.5209 mL	1.0418 mL	2.6044 mL
	15 mM	0.0695 mL	0.3473 mL	0.6945 mL	1.7363 mL
	20 mM	0.0521 mL	0.2604 mL	0.5209 mL	1.3022 mL
	25 mM	0.0417 mL	0.2084 mL	0.4167 mL	1.0418 mL

DiD perchlorate Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DiD127274-91-3Fluorescent DyeDildialkylcarbocyaninesanalogInhibitorinhibitorinhibit

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DiD perchlorate

Customer Review

Other Forms of DiD perchlorate:

DiD perchlorate Related Antibodies

36 Publications Citing Use of MCE DiD perchlorate

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

DiD perchlorate Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

DiD perchlorate Related Classifications

Keywords:

Your Recently Viewed Products:

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