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DiD

(Synonyms: Cy5 DIC18)
45 Cited Publications
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Based on 45 publication(s) in Google Scholar

DiD is a long-chain carbocyanine dye. Carbocyanine dyes are widely used as Di to label cells, organelles, liposomes, viruses and lipoproteins (Ex/Em = 633/665 nm) .

For research use only. We do not sell to patients.

  • CAS No.: 75539-51-4
  • Formula: C61H99IN2
  • Molecular Weight:987.36
  • Storage:

    Please store the product under the recommended conditions in the Certificate of Analysis.

  • DiD Chemical Structure
    DiD Chemical Structure
  • Experimental Validation Results for DiD
  • Experimental Validation Results for DiD
  • Experimental Validation Results for DiD
  • Experimental Validation Results for DiD
  • Experimental Validation Results for DiD
CAS No.: 75539-51-4
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Publications Citing Use of MedChemExpress (MCE) DiD

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Cell Imaging/StainingIn Vivo Imaging
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  • Cell Imaging/Staining
  • Cell Imaging/Staining
  • Cell Imaging/Staining
  • In Vivo Imaging
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  • DiD Chemical Structure
    DiD Chemical Structure
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    DiD purchased from MedChemExpress. Usage Cited in: Nature. 2025 Nov;647(8089):506-516. [Abstract]

    Customer Validation
     Primary macrophages were isolated as described above. After staining 4T1 cells with DID (MCE, HY-D1028) and macrophages with CFSE, cells were cultured with an effector-to-target ratio of 3:1 at 37 °C for 24 h. The phagocytosis effect was visualized by confocal imaging. 4T1 cells and macrophages were visualized by DiD dye (magenta) and CFSE dye (green).

  • 1/16

    DiD purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10096. [Abstract]

    Customer Validation
     Fluorescence images of cellular uptake in Huh7, THLE3, PANC1, M2, M1, and CM after Sv@PM-M2p treatment (Scale bar = 100 μm), along with the quantification of red fluorescence intensity (n = 3 independent experiments). Sv@PM-M2p was labeled with DiD (red), the nuclei and cytoskeletons were stained with DAPI (blue) and Phalloidin (green), respectively.

  • 1/16

    DiD purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10096. [Abstract]

    Customer Validation
     Fluorescence images of cellular uptake in the Huh7 and M2 co-culture system after Sv@PM-M2p treatment (Scale bar = 100 μm), along with the quantification of red fluorescence intensity (n = 3 independent experiments). Sv@PM-M2p was labeled with DiD (red), the nuclei and cytoskeletons were stained with DAPI (blue) and Phalloidin (green), respectively.

  • 1/16

    DiD purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Oct 14;42(10):1729-1746.e8. [Abstract]

    Customer Validation
     FITC-labeled butyrate translocated to the nucleus of CD8+ T cells after 6 h of co-culture, as determined by confocal microscopy. CD8+ T cells were stained using FITC-labeled butyrate (green), DiD perchlorate (red), and DAPI (blue).

  • 1/16

    DiD purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Oct;11(38):e2402329. [Abstract]

    Customer Validation
     Fluorescence imaging was performed on subcutaneous tumor-bearing mice after being injected with DiD-labeled LNPs through the tail vein.

  • DiD