DiD perchlorate (solution)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
General Protocol
1. Preparing Stain Solutions of Di
1) Prepare working solutions: Dilute the stock solutions into a suitable buffer such as serum-free culture medium, HBSS or PBS to make 1 to 5 μM working solutions. We do not recommend storing the aqueous solution for more than one day.
Note: The final concentration of the working solution should be empirically determined for different cell types and/or experimental conditions.
2. Suspension cells
1) Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10⁶/mL
2) Add 1 mL of Di working solution, and then incubate at room temperature for 5-30 minutes.
3) Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
4) Wash twice with PBS, 5 minutes each time.
5) Resuspend cells with serum-free cell culture medium or PBS.Observation by fluorescence microscopy or flow cytometry.
3. Adherent cells
1) Culture adherent cells on sterile coverslips.
2) Remove the coverslip from the medium and aspirate the excess medium.
3) Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
4) Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.