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DiI (Synonyms: DiIC18(3))

Cat. No.: HY-D0083 Purity: 99.92%
Handling Instructions

DiI (DiIC18(3)) is a lipophilic carbocyanine fluorescent dye for membrane labeling. DiI has an absorption maximum at 549 nm and an emission maximum 565 nm. DiI is mildly fluorescent in aqueous suspension, but becomes bright when bound to cell membrane.

For research use only. We do not sell to patients.

DiI Chemical Structure

DiI Chemical Structure

CAS No. : 41085-99-8

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
10 mg USD 60 In-stock
Estimated Time of Arrival: December 31
25 mg USD 88 In-stock
Estimated Time of Arrival: December 31
50 mg USD 120 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 7 publication(s) in Google Scholar

Top Publications Citing Use of Products

    DiI purchased from MCE. Usage Cited in: J Immunother Cancer. 2020 Aug;8(2):e000330.

    Ecto-CRT translocation to cell surfaces confirmed by three counterstains and merged analysis with an anti-CRT antibody followed by FITG-conjugated secondary antibody (green), DiI (red), and 4',6-diamidino-2-phenylindole (DAPI, blue).
    • Biological Activity

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    Description

    DiI (DiIC18(3)) is a lipophilic carbocyanine fluorescent dye for membrane labeling. DiI has an absorption maximum at 549 nm and an emission maximum 565 nm. DiI is mildly fluorescent in aqueous suspension, but becomes bright when bound to cell membrane.

    In Vitro

    Once bound to a membrane it diffuses laterally in 2 dimensions; if there is no diffusion barrier, DiI generally stains the whole leaflet (one surface) of a biological membrane rapidly, but does not readily flip across to the other leaflet.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    DiI-labeled motoneurons have remained viable for up to 4 weeks in culture and up to one year in vivo[1]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    933.87

    Formula

    C₅₉H₉₇ClN₂O₄

    CAS No.

    41085-99-8

    SMILES

    CCCCCCCCCCCCCCCCCC[N+]1=C(/C=C/C=C2N(CCCCCCCCCCCCCCCCCC)C3=C(C=CC=C3)C/2(C)C)C(C)(C)C4=C1C=CC=C4.O=Cl(=O)([O-])=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 16.67 mg/mL (17.85 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.0708 mL 5.3541 mL 10.7081 mL
    5 mM 0.2142 mL 1.0708 mL 2.1416 mL
    10 mM 0.1071 mL 0.5354 mL 1.0708 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: 2.5 mg/mL (2.68 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 2.5 mg/mL (2.68 mM); Suspended solution; Need ultrasonic

    *All of the co-solvents are provided by MCE.

    Purity: 99.92%

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    Keywords:

    DiIDiIC18(3)OthersInhibitorinhibitorinhibit

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    Product Name:
    DiI
    Cat. No.:
    HY-D0083
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