DiI (solution) 

DiI (solution) is a long-chain carbocyanine dye. Carbocyanine dyes are widely used as Di to label cells, organelles, liposomes, viruses and lipoproteins^[2].
Solvent and concentration: DMSO: 5 mM

Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
1. Preparation solution: Dilute the reserve solution to a suitable buffer such as serum-free medium, HBSS, or PBS to prepare a working solution of 1 to 5 μM.
2. Suspension cells
a. Centrifuge at 1000g for 3-5 minutes at 4°C, discard the supernatant, wash twice with PBS, each time for 5 minutes, and the cell density is 1×10⁶/mL.
b. Add 1 mL of DiI working solution, incubate at room temperature for 5-30 minutes.
C. Centrifuge at 4°C, 400g for 3-4 minutes, discard the supernatant.
d. Wash twice with PBS, each time for 5 minutes.
e. Resuspend the cells in serum-free cell culture medium or PBS, and observe with a fluorescence microscope or flow cytometer.
3. Adherent cells
a. Cultivate adherent cells on a sterile cover glass.
b. Remove the cover glass from the culture medium and aspirate the excess medium.
c. Add 100 μL of the working solution, gently shake to fully cover the cells, incubate at room temperature for 5-30 minutes.
d. Wash twice with the culture medium, each time for 5 minutes. Observe through a fluorescence microscope or flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DiI-labeled motoneurons have remained viable for up to 4 weeks in culture and up to one year in vivo^[1]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

933.89

C₅₉H₉₇ClN₂O₄

41085-99-8

Liquid

Purple to purplish red

CCCCCCCCCCCCCCCCCC[N+]1=C(/C=C/C=C2N(CCCCCCCCCCCCCCCCCC)C3=C(C=CC=C3)C/2(C)C)C(C)(C)C4=C1C=CC=C4.O=Cl(=O)([O-])=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. D P Kuffler, et al. Long-term survival and sprouting in culture by motoneurons isolated from the spinal cord of adult frogs. J Comp Neurol. 1990 Dec 22;302(4):729-38.  [Content Brief]

  [2]. Gan WB, et al. Multicolor "DiOlistic" labeling of the nervous system using lipophilic dye combinations. Neuron. 2000 Aug;27(2):219-25.  [Content Brief]

  [3]. D P Kuffler, et al. Long-term survival and sprouting in culture by motoneurons isolated from the spinal cord of adult frogs. J Comp Neurol. 1990 Dec 22;302(4):729-38.  [Content Brief]