Digoxigenin (Synonyms: Lanadigenin)

Name: Digoxigenin
Brand: MedChemExpress
SKU: HY-B1025
Rating: 4.5 (1 reviews)

Cat. No.: HY-B1025 Purity: 99.60%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Digoxigenin (DIG) is a steroid. DIG is used for situ hybridization as a labeling molecule probe due to long shelf life and fast detection and high sensitivity of DIG-labeled riboprobes.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Digoxigenin 화학구조

CAS No. : 1672-46-4

사이즈	재고	수량

무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	견적 받기
200 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 publication(s) in Google Scholar

Other Forms of Digoxigenin:

Digoxigenin-d₃ 견적 받기
Digoxigenin (Standard) 견적 받기

1 Publications Citing Use of MCE Digoxigenin

•J Pharm Biomed Anal. 2025 Dec 15:266:117118. [Abstract]

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
순도&문서
References
고객리뷰

제품 설명

Digoxigenin (DIG) is a steroid. DIG is used for situ hybridization as a labeling molecule probe due to long shelf life and fast detection and high sensitivity of DIG-labeled riboprobes^[1]^[3].

Cellular Effect

Cell Line	Type	Value	Description	References
HT-29	IC₅₀	3.6 μM Compound: 5	Cytotoxicity against human HT-29 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay Cytotoxicity against human HT-29 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay	[PMID: 32096998]
MCF7	IC₅₀	507 nM Compound: 4	Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay	[PMID: 16309315]
MDA-MB-231	IC₅₀	3.2 μM Compound: 5	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay	[PMID: 32096998]
MDA-MB-435	IC₅₀	0.9 μM Compound: 5	Cytotoxicity against human MDA-MB-435 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay Cytotoxicity against human MDA-MB-435 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay	[PMID: 32096998]
MDCK	IC₅₀	0.49 μM Compound: Digoxigenin	TP_TRANSPORTER: inhibition of Digoxin uptake in OATP4C1-expressing MDCK cells TP_TRANSPORTER: inhibition of Digoxin uptake in OATP4C1-expressing MDCK cells	[PMID: 14993604]
OVCAR-3	IC₅₀	2.4 μM Compound: 5	Cytotoxicity against human OVCAR3 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay Cytotoxicity against human OVCAR3 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay	[PMID: 32096998]
TK-10	IC₅₀	317.5 nM Compound: 4	Cytotoxicity against human TK10 cells after 48 hrs by SRB assay Cytotoxicity against human TK10 cells after 48 hrs by SRB assay	[PMID: 16309315]
UACC-62	IC₅₀	416.1 nM Compound: 4	Cytotoxicity against human UACC62 cells after 48 hrs by SRB assay Cytotoxicity against human UACC62 cells after 48 hrs by SRB assay	[PMID: 16309315]

In Vitro

Digoxigenin is a potent activator of RORγ-dependent transcription at 1 μM with an EC₅₀ value of 0.358 μM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	HepG2 (human hepatocellular carcinoma) cell line
Concentration:	0-10 μM
Incubation Time:	24 h
Result:	Identified the optimal concentration for Digoxigenin: 1 μM.

Cell Cytotoxicity Assay^[2]

Cell Line:	RORγ-HepG2 cells
Concentration:	0-2 μM
Incubation Time:	24 h
Result:	Increased the luciferase activity with concentration-dependent in cells.

분자량

390.51

화학식

C₂₃H₃₄O₅

CAS No.

1672-46-4

Appearance

Solid

Color

White to off-white

SMILES

O=C1OCC([C@H]2CC[C@]3(O)[C@]4([H])CC[C@]5([H])C[C@@H](O)CC[C@]5(C)[C@@]4([H])C[C@@H](O)[C@]23C)=C1

Structure Classification

Steroids

Initial Source

선적

Room temperature in continental US; may vary elsewhere.

보관

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

용액&용해도

In Vitro:

DMSO : 100 mg/mL (256.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5608 mL	12.8038 mL	25.6075 mL
5 mM	0.5122 mL	2.5608 mL	5.1215 mL
10 mM	0.2561 mL	1.2804 mL	2.5608 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

순도&문서

Purity: 99.70%

Select Batch:

Data Sheet (278 KB) SDS (420 KB)

COA (254 KB) LCMS (289 KB)

Handling Instructions (2659 KB)

References

[1]. Luo, Yuhao, et al. Long noncoding RNA (lncRNA) EIF3J-DT induces chemoresistance of gastric cancer via autophagy activation. Autophagy vol. 17,12 (2021): 4083-4101. [Content Brief]

[2]. Karaś, Kaja, et al. The cardenolides strophanthidin, digoxigenin and dihydroouabain act as activators of the human RORγ/RORγT receptors. Toxicology letters vol. 295 (2018): 314-324. [Content Brief]

[3]. Barratt, Kristen S, et al. Production of Digoxigenin-Labeled Riboprobes for In Situ Hybridization Experiments. Current protocols in mouse biology vol. 10,2 (2020): e74. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5608 mL	12.8038 mL	25.6075 mL	64.0188 mL
	5 mM	0.5122 mL	2.5608 mL	5.1215 mL	12.8038 mL
	10 mM	0.2561 mL	1.2804 mL	2.5608 mL	6.4019 mL
	15 mM	0.1707 mL	0.8536 mL	1.7072 mL	4.2679 mL
	20 mM	0.1280 mL	0.6402 mL	1.2804 mL	3.2009 mL
	25 mM	0.1024 mL	0.5122 mL	1.0243 mL	2.5608 mL
	30 mM	0.0854 mL	0.4268 mL	0.8536 mL	2.1340 mL
	40 mM	0.0640 mL	0.3201 mL	0.6402 mL	1.6005 mL
	50 mM	0.0512 mL	0.2561 mL	0.5122 mL	1.2804 mL
	60 mM	0.0427 mL	0.2134 mL	0.4268 mL	1.0670 mL
	80 mM	0.0320 mL	0.1600 mL	0.3201 mL	0.8002 mL
	100 mM	0.0256 mL	0.1280 mL	0.2561 mL	0.6402 mL

Digoxigenin Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Digoxigenin1672-46-4LanadigeninFluorescent Dyesitu hybridizationprobeInhibitorinhibitorinhibit

최근 본 상품:

상품명

Requested Quantity *

고객명 *

호칭

메일주소 *

전화번호 *

Department

회사명 *

City

도

Country or Region *

비고

상품명

Lot #

고객명 *

메일주소 *

전화번호

Department *

회사명 *

Country or Region *

비고

Name
이메일 *

	Sorry, but the email address you supplied was invalid.

Digoxigenin (Synonyms: Lanadigenin)

고객리뷰

Other Forms of Digoxigenin:

Digoxigenin Related Antibodies

1 Publications Citing Use of MCE Digoxigenin

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

순도&문서

References

고객리뷰

Digoxigenin Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Digoxigenin Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report