GSK2795039
Based on 76 publication(s) in Google Scholar
GSK2795039 is a NADPH oxidase 2 (NOX2) inhibitor with a mean pIC50 of 6 in different cell-free assays. GSK2795039 inhibits reactive oxygen species (ROS) production and NADPH consumption. GSK2795039 reduces apoptosis.
For research use only. We do not sell to patients.
- Purity: 98.56%
- CAS No.: 1415925-18-6
- Formula: C23H26N6O2S
- Molecular Weight:450.56
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Immunology/Inflammation Compound Library
HY-L007
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Metabolism/Protease Compound Library
HY-L012
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NF-κB Signaling Compound Library
HY-L014
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Anti-Cancer Compound Library
HY-L025
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Anti-Aging Compound Library
HY-L034
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Antioxidant Compound Library
HY-L037
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Oxygen Sensing Compound Library
HY-L045
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Ferroptosis Compound Library
HY-L051
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Glutamine Metabolism Compound Library
HY-L064
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Anti-Cancer Metabolism Compound Library
HY-L083
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Glucose Metabolism Compound Library
HY-L092
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Targeted Diversity Library
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Anti-Pulmonary Fibrosis Compound Library
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Heterocyclic Compound Library
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Metabolic Enzyme Compound Library
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MCE Bioactive Compound Library
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
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Lactylation Compound Library
HY-L249
Publications Citing Use of MedChemExpress (MCE) GSK2795039
More- Nat Immunol. 2021 Sep;22(9):1107-1117. [Abstract]
- Cell Host Microbe. 2022 Oct 12;30(10):1450-1463.e8. [Abstract]
- ACS Nano. 2022 Nov 22;16(11):18886-18897. [Abstract]
- Nat Commun. 2025 Feb 13;16(1):1593. [Abstract]
- Nat Commun. 2020 Mar 6;11(1):1242. [Abstract]
- J Extracell Vesicles. 2020 May 4;9(1):1757900. [Abstract]
- J Clin Invest. 2022 Feb 1;132(3):e146343. [Abstract]
- Theranostics. 2021 Jan 30;11(8):3796-3812. [Abstract]
- Theranostics. 2020 Sep 2;10(24):11013-11025. [Abstract]
- Sci Adv. 2025 Oct 3;11(40):eady9234. [Abstract]
- Redox Biol. 2023 Aug:64:102763. [Abstract]
- Redox Biol. 2020 May;32:101466. [Abstract]
- Redox Biol. 2019 Oct:27:101075. [Abstract]
- J Hazard Mater. 2021 Aug 15:416:126158. [Abstract]
- Int J Biol Sci. 2024 Apr 29;20(7):2658-2685. [Abstract]
- J Pharm Anal. 2025 May;15(5):101093. [Abstract]
- Acta Pharmacol Sin. 2023 Oct;44(10):2125-2138. [Abstract]
- Free Radic Biol Med. 2021 Aug 1:171:219-231. [Abstract]
- Free Radical Bio Med. 2021 May 20:168:214-225. [Abstract]
- Cancer Immunol Res. 2020 Apr;8(4):436-450. [Abstract]
- Ann Neurol. 2019 Jun;85(6):907-920. [Abstract]
- Redox Rep. 2025 Dec;30(1):2494314. [Abstract]
- Blood Adv. 2021 Dec 14;5(23):5439-5451. [Abstract]
- Cell Rep. 2019 Dec 24;29(13):4435-4446.e9. [Abstract]
- Neural Regen Res. 2021 Aug;16(8):1582-1591. [Abstract]
- Antioxidants (Basel). 2022 Dec 30;12(1):92. [Abstract]
- Antioxidants (Basel). 2021 Jan 14;10(1):109. [Abstract]
- NPJ Regen Med. 2024 Oct 2;9(1):28. [Abstract]
- Antioxid Redox Signal. 2024 Sep;41(7-9):488-504. [Abstract]
- Biochem Pharmacol. 2023 Dec:218:115934. [Abstract]
- Chem Biol Interact. 2024 May 31:111091. [Abstract]
- Commun Biol. 2021 Sep 9;4(1):1054. [Abstract]
- CNS Neurosci Ther. 2025 Nov;31(11):e70638. [Abstract]
- Int J Mol Sci. 2026 Mar 13;27(6):2646. [Abstract]
- Int J Mol Sci. 2025 Apr 12;26(8):3647. [Abstract]
- Int J Mol Sci. 2020 May 26;21(11):3750. [Abstract]
- Pharmaceuticals (Basel). 2026 Mar 11;19(3):452. [Abstract]
- Front Pharmacol. 2021 Sep 28:12:751845. [Abstract]
- Eur J Pharmacol. 2025 Jun 15:997:177483. [Abstract]
- Int Immunopharmacol. 2024 Oct 29;143(Pt 2):113504. [Abstract]
- Eur J Pharmacol. 2024 Mar 15:967:176351. [Abstract]
- Int Immunopharmacol. 2022 Dec;113(Pt A):109321. [Abstract]
- Int Immunopharmacol. 2021 Jul:96:107774. [Abstract]
- Mbio. 2021 Mar 2;12(2):e03305-20. [Abstract]
- Immunotargets Ther. 2026 Jun 6:15:596703. [Abstract]
- J Hypertens. 2024 Apr 23. [Abstract]
- Cell Calcium. 2026 Mar:134:103122. [Abstract]
- Sci Rep. 2025 Oct 16;15(1):36270. [Abstract]
- Biomedicines. 2023 Jan 31;11(2):416. [Abstract]
- Sci Rep. 2019 Dec 11;9(1):18852. [Abstract]
- Br J Haematol. 2021 Jan;192(1):137-145. [Abstract]
- Neurochem Res. 2020 May;45(5):1097-1106. [Abstract]
- Mol Med Rep. 2024 May;29(5):84. [Abstract]
- Toxicol Appl Pharmacol. 2023 Mar 15:463:116412. [Abstract]
- J Immunol. 2021 Aug 1;207(3):966-973. [Abstract]
- J Immunol. 2021 Jul 1;207(1):296-307. [Abstract]
- Cancer Res Commun. 2024 Apr 10;4(4):1135-1149. [Abstract]
- Endocrinology. 2019 Apr 1;160(4):947-963. [Abstract]
- PLoS One. 2022 Jan 24;17(1):e0263080. [Abstract]
- J Nat Med. 2025 Jul;79(4):791-806. [Abstract]
- Biochem Biophys Res Commun. 2022 Aug 20;617(Pt 1):1-7. [Abstract]
- Bio Protoc. 2020 Sep 5;10(17):e3737. [Abstract]
- bioRxiv. 2026 Jun 15.
- bioRxiv. 2026 Jun 23.
- Res Sq. 2026 May 20.
- bioRxiv. 2025 May 21.
- SSRN. 2025 Jan 23.
- hLife. 2025 Jan 25.
- bioRxiv. 2024 Apr 26:2024.04.21.590456. [Abstract]
- Universidad De Salamanca. 2023
- University of Dublin. 2022.
- Oxid Med Cell Longev. 2020 Jul 19;2020:2930463. [Abstract]
- Research Square Preprint. 2020 Jun.
- Università degli studi di Milano. 2020 May.
- Immunohorizons. 2019 Oct 18;3(10):488-497. [Abstract]
- Oxid Med Cell Longev. 2019 Apr 28:2019:2717986. [Abstract]
Customer Validation & Images
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WB
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IF
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WB
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Cell Imaging/Staining
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RT-PCR
Biological Activity
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NOX2 |
GSK2795039 (25 μM; 24 hours) reduces the combinatory effect of FeSO4 and LPS-increased levels of apoptosis and reduced the presence of caspase-3-positive PC12 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PC12 cells
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Concentration:25 μM
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Incubation Time:24 hours
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Result:Reduced apoptosis among PC12 cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL6 mice[1]
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Dosage:100 mg/kg
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Administration:Intraperitoneal injection; 100 mg/kg; 1 hour before
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Result:Caused 50% reduction in the level of serum amylase activity.
Chemical Information
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CAS No. 1415925-18-6
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Appearance Solid
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Molecular Weight 450.56
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Formula C23H26N6O2S
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Color White to yellow
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SMILES
CC(C)N1C=C(C2=CC=C(CCN3C)C3=C2)C4=C1N=CC=C4NS(C5=NN(C)C=C5)(=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (76)
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Journal Impact Factor
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Most Recent
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Nat Immunol
2021 Sep;22(9):1107-1117. PMID: 34385713
GSK2795039 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2021 Sep;22(9):1107-1117. [Abstract]
Western blot analysis of GPX4 expression in HC neutrophils (n=3) when cultured with 20% HC serum or SLE serum supplemented with GSK2795039 (NOX2 inhibitor, 10 μM, 24h and 72h).
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Cell Host Microbe
Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation. [Abstract]2022 Oct 12;30(10):1450-1463.e8. PMID: 36228585
GSK2795039 purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2022 Oct 12;30(10):1450-1463.e8. [Abstract]
TNF-α (50 ng/mL; 6 h); GSK2795039 (25 μM; 6 h).NETs were detected using immunofluorescent staining of DNA (DAPI, blue), dead cells and extracellular DNA (Sytox, green), histone 3 (pink), and myeloperoxidase (MPO, red). Scale bars, 50 μm.
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ACS Nano
Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia. [Abstract]2022 Nov 22;16(11):18886-18897. PMID: 36288210 -
Nat Commun
The systemic lupus erythematosus-associated NCF190H allele synergizes with viral infection to cause mouse lupus but also limits virus spread. [Abstract]2025 Feb 13;16(1):1593. PMID: 39939342
GSK2795039 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 13;16(1):1593. [Abstract]
Western blots of p-JAK1, p-STAT1, and p-STAT3 in IFN-α stimulated macrophages, together with GSK2795039 (25 µM) in BMDMs (M0 cells).
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Nat Commun
CCN1 is an opsonin for bacterial clearance and a direct activator of Toll-like receptor signaling. [Abstract]2020 Mar 6;11(1):1242. PMID: 32144270
GSK2795039 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Mar 6;11(1):1242. [Abstract]
The effects of NOX2 inhibitor GSK2795039 (10 µM) on CCN1-induced ROS production. GSK2795039 was added 30 min prior to CCN1 treatment.
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J Extracell Vesicles
CD73+ extracellular vesicles inhibit angiogenesis through adenosine A2B receptor signalling. [Abstract]2020 May 4;9(1):1757900. PMID: 32489531 -
J Clin Invest
Targeting the ASMase/S1P pathway protects from sortilin-evoked vascular damage in hypertension. [Abstract]2022 Feb 1;132(3):e146343. PMID: 35104805 -
Theranostics
Impairment of type H vessels by NOX2-mediated endothelial oxidative stress: critical mechanisms and therapeutic targets for bone fragility in streptozotocin-induced type 1 diabetic mice. [Abstract]2021 Jan 30;11(8):3796-3812. PMID: 33664862 -
Theranostics
Toll-like receptor 5 deficiency diminishes doxorubicin-induced acute cardiotoxicity in mice. [Abstract]2020 Sep 2;10(24):11013-11025. PMID: 33042267 -
Sci Adv
Mitochondrial ROS trigger interorganellular signaling and prime ER processes to establish enhanced plant immunity. [Abstract]2025 Oct 3;11(40):eady9234. PMID: 41032619 -
Redox Biol
NOX2 inhibition stabilizes vulnerable plaques by enhancing macrophage efferocytosis via MertK/PI3K/AKT pathway. [Abstract]2023 Aug:64:102763. PMID: 37354827 -
Redox Biol
A closer look into NADPH oxidase inhibitors: Validation and insight into their mechanism of action. [Abstract]2020 May;32:101466. PMID: 32105983 -
Redox Biol
Glucose-dependent trans-plasma membrane electron transport and p70S6k phosphorylation in skeletal muscle cells. [Abstract]2019 Oct:27:101075. PMID: 30578122 -
J Hazard Mater
Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells. [Abstract]2021 Aug 15:416:126158. PMID: 34492938 -
Int J Biol Sci
Mitochondrial Dysfunction by FADDosome Promotes Gastric Mucosal Injury in Portal Hypertensive Gastropathy. [Abstract]2024 Apr 29;20(7):2658-2685. PMID: 38725851 -
J Pharm Anal
Liquiritin improves macrophage degradation of engulfed tumour cells by promoting the formation of phagolysosomes via NOX2/gp91phox. [Abstract]2025 May;15(5):101093. PMID: 40496062 -
Acta Pharmacol Sin
Activated SIRT1 contributes to DPT-induced glioma cell parthanatos by upregulation of NOX2 and NAT10. [Abstract]2023 Oct;44(10):2125-2138. PMID: 37277492 -
Free Radic Biol Med
2021 Aug 1:171:219-231. PMID: 34010663 -
Free Radical Bio Med
Detection of extracellular superoxide in isolated human immune cells and in an animal model of arterial hypertension using hydropropidine probe and HPLC analysis. [Abstract]2021 May 20:168:214-225. PMID: 33823245 -
Cancer Immunol Res
Cancer-Associated Fibroblasts Promote Immunosuppression by Inducing ROS-Generating Monocytic MDSCs in Lung Squamous Cell Carcinoma. [Abstract]2020 Apr;8(4):436-450. PMID: 32075803 -
Ann Neurol
Activation of nicotinamide adenine dinucleotide phosphate oxidase is the primary trigger of epileptic seizures in rodent models. [Abstract]2019 Jun;85(6):907-920. PMID: 30937971 -
Redox Rep
Norepinephrine promotes oxidative stress in vascular adventitial fibroblasts via PKC/NFκB-mediated NOX2 upregulation. [Abstract]2025 Dec;30(1):2494314. PMID: 40269356 -
Blood Adv
Inhibition of NADPH oxidase blocks NETosis and reduces thrombosis in heparin-induced thrombocytopenia. [Abstract]2021 Dec 14;5(23):5439-5451. PMID: 34478504 -
Cell Rep
Dectin-1 Binding to Annexins on Apoptotic Cells Induces Peripheral Immune Tolerance via NADPH Oxidase-2. [Abstract]2019 Dec 24;29(13):4435-4446.e9. PMID: 31875551
GSK2795039 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2019 Dec 24;29(13):4435-4446.e9. [Abstract]
GSK2795039 (2 μM) blocks annexin-induced ROSs.
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Neural Regen Res
P2X7 receptor activation aggravates NADPH oxidase 2-induced oxidative stress after intracerebral hemorrhage. [Abstract]2021 Aug;16(8):1582-1591. PMID: 33433488 -
Antioxidants (Basel)
Selective Pharmacological Inhibition of NOX2 by GSK2795039 Improves Bladder Dysfunction in Cyclophosphamide-Induced Cystitis in Mice. [Abstract]2022 Dec 30;12(1):92. PMID: 36670953 -
Antioxidants (Basel)
Investigations into the Role of Metabolism in the Inflammatory Response of BV2 Microglial Cells. [Abstract]2021 Jan 14;10(1):109. PMID: 33466581 -
NPJ Regen Med
Infected wound repair correlates with collagen I induction and NOX2 activation by cold atmospheric plasma. [Abstract]2024 Oct 2;9(1):28. PMID: 39358383 -
Antioxid Redox Signal
Intervention of asprosin attenuates oxidative stress and neointima formation in vascular injury. [Abstract]2024 Sep;41(7-9):488-504. PMID: 38814824 -
Biochem Pharmacol
Hydroquinone-selected chronic myelogenous leukemia cells are sensitive to chloroquine-induced cytotoxicity via MCL1 suppression and glycolysis inhibition. [Abstract]2023 Dec:218:115934. PMID: 37989415 -
Chem Biol Interact
In vivo upstream factors of mouse hepatotoxic mechanism with sustained hepatic glutathione depletion: Acetaminophen metabolite-erythrocyte adducts and splenic macrophage-generated reactive oxygen species. [Abstract]2024 May 31:111091. PMID: 38825056 -
Commun Biol
Aβ initiates brain hypometabolism, network dysfunction and behavioral abnormalities via NOX2-induced oxidative stress in mice. [Abstract]2021 Sep 9;4(1):1054. PMID: 34504272 -
CNS Neurosci Ther
Microglial SLC25A28 Knockout Mitigates Spinal Cord Injury in Mice by Inhibiting Heme Synthesis and Subsequent NOX2 Activation. [Abstract]2025 Nov;31(11):e70638. PMID: 41177991 -
Int J Mol Sci
LRRC8A Inhibition Overcomes Chemoresistance by Downregulating MRP3 and CYP3A4 in the 3D Spheroid Model of Human Breast Cancer Cells. [Abstract]2026 Mar 13;27(6):2646. PMID: 41898509 -
Int J Mol Sci
Improved Synthesis of Effective 3-(Indolin-6-yl)-4-(N-pyrazole-sulfonamide)-1 H-pyrrolo[2,3- b]pyridine-Based Inhibitors of NADPH Oxidase 2. [Abstract]2025 Apr 12;26(8):3647. PMID: 40332246 -
Int J Mol Sci
In Vivo Transmigrated Human Neutrophils Are Highly Primed for Intracellular Radical Production Induced by Monosodium Urate Crystals. [Abstract]2020 May 26;21(11):3750. PMID: 32466527 -
Pharmaceuticals (Basel)
Xu Chunfu's Modified Xianglian Pill Regulates the NOX2/ROS/Mitochondria/NLRP3 Axis to Treat Ulcerative Colitis. [Abstract]2026 Mar 11;19(3):452. PMID: 41901297 -
Front Pharmacol
Nox4 Promotes RANKL-Induced Autophagy and Osteoclastogenesis via Activating ROS/PERK/eIF-2α/ATF4 Pathway. [Abstract]2021 Sep 28:12:751845. PMID: 34650437 -
Eur J Pharmacol
NADPH oxidase 2 inhibitor GSK2795039 prevents against cardiac remodeling after MI through reducing oxidative stress and mitochondrial dysfunction. [Abstract]2025 Jun 15:997:177483. PMID: 40057160 -
Int Immunopharmacol
NOX2 deficiency promotes GSDME-related pyroptosis by reducing AMPK activation in neutrophils. [Abstract]2024 Oct 29;143(Pt 2):113504. PMID: 39476568 -
Eur J Pharmacol
NADPH oxidase 2 inhibitor GSK2795039 prevents doxorubicin-induced cardiac atrophy by attenuating cardiac sympathetic nerve terminal abnormalities and myocyte autophagy. [Abstract]2024 Mar 15:967:176351. PMID: 38290568 -
Int Immunopharmacol
Ursolic acid alleviates Kupffer cells pyroptosis in liver fibrosis by the NOX2/NLRP3 inflammasome signaling pathway. [Abstract]2022 Dec;113(Pt A):109321. PMID: 36252479 -
Int Immunopharmacol
TLR4/TRAF6/NOX2 signaling pathway is involved in ventilation-induced lung injury via endoplasmic reticulum stress in murine model. [Abstract]2021 Jul:96:107774. PMID: 34020396 -
Mbio
ROP16-Mediated Activation of STAT6 Suppresses Host Cell Reactive Oxygen Species Production, Facilitating Type III Toxoplasma gondii Growth and Survival. [Abstract]2021 Mar 2;12(2):e03305-20. PMID: 33653884 -
Immunotargets Ther
Myeloid Cell-Derived Thrombospondin-1 Mediates Anti-Metastatic Effects of Histamine Dihydrochloride in B16 Melanoma. [Abstract]2026 Jun 6:15:596703. PMID: 42281617 -
J Hypertens
Asprosin contributes to vascular remodeling in hypertensive rats via superoxide signaling. [Abstract]2024 Apr 23. PMID: 38690935 -
Cell Calcium
A NOX2-independent mechanism of Hv1 channel activation promotes inflammatory cytokine release from BV-2 microglia via intracellular Ca2+ mobilisation. [Abstract]2026 Mar:134:103122. PMID: 41581267 -
Sci Rep
2025 Oct 16;15(1):36270. PMID: 41102391 -
Biomedicines
Effects of NADPH Oxidase Isoform-2 (NOX2) Inhibition on Behavioral Responses and Neuroinflammation in a Mouse Model of Neuropathic Pain. [Abstract]2023 Jan 31;11(2):416. PMID: 36830952 -
Sci Rep
VAS2870 and VAS3947 attenuate platelet activation and thrombus formation via a NOX-independent pathway downstream of PKC. [Abstract]2019 Dec 11;9(1):18852. PMID: 31827142 -
Br J Haematol
Identification of genetic targets in acute myeloid leukaemia for designing targeted therapy. [Abstract]2021 Jan;192(1):137-145. PMID: 33022753 -
Neurochem Res
An Effective NADPH Oxidase 2 Inhibitor Provides Neuroprotection and Improves Functional Outcomes in Animal Model of Traumatic Brain Injury. [Abstract]2020 May;45(5):1097-1106. PMID: 32072445 -
Mol Med Rep
Food therapy of scutellarein ameliorates pirarubicin‑induced cardiotoxicity in rats by inhibiting apoptosis and ferroptosis through regulation of NOX2‑induced oxidative stress. [Abstract]2024 May;29(5):84. PMID: 38516760 -
Toxicol Appl Pharmacol
GSK2795039 prevents RIP1-RIP3-MLKL-mediated cardiomyocyte necroptosis in doxorubicin-induced heart failure through inhibition of NADPH oxidase-derived oxidative stress. [Abstract]2023 Mar 15:463:116412. PMID: 36764612 -
J Immunol
Lipoproteins from Staphylococcus aureus Drive Neutrophil Extracellular Trap Formation in a TLR2/1- and PAD-Dependent Manner. [Abstract]2021 Aug 1;207(3):966-973. PMID: 34290104 -
J Immunol
Neddylation Alleviates Methicillin-Resistant Staphylococcus aureus Infection by Inducing Macrophage Reactive Oxygen Species Production. [Abstract]2021 Jul 1;207(1):296-307. PMID: 34183370 -
Cancer Res Commun
Impact of Surgery-Induced Myeloid-derived Suppressor Cells and the NOX2/ROS Axis on Postoperative Survival in Human Pancreatic Cancer. [Abstract]2024 Apr 10;4(4):1135-1149. PMID: 38598844 -
Endocrinology
2019 Apr 1;160(4):947-963. PMID: 30811529 -
PLoS One
Repeated glucose spikes and insulin resistance synergistically deteriorate endothelial function and bardoxolone methyl ameliorates endothelial dysfunction. [Abstract]2022 Jan 24;17(1):e0263080. PMID: 35073378 -
J Nat Med
Ginsenoside Rh3-induced neurotoxicity involving the IP3R-Ca2+/NOX2/NF-κB signaling pathways. [Abstract]2025 Jul;79(4):791-806. PMID: 40360886 -
Biochem Biophys Res Commun
Inhibition of NADPH oxidase 2 (NOX2) reverses cognitive deficits by modulating excitability and excitatory transmission in the hippocampus after traumatic brain injury. [Abstract]2022 Aug 20;617(Pt 1):1-7. PMID: 35660876 -
Bio Protoc
Flow-cytometric Detection of Low-level Reactive Oxygen Species in Cell Lines and Primary Immune Cells. [Abstract]2020 Sep 5;10(17):e3737. PMID: 33659398 -
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bioRxiv
2024 Apr 26:2024.04.21.590456. PMID: 38712240 -
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Oxid Med Cell Longev
Dexmedetomidine Attenuates LPS-Induced Monocyte-Endothelial Adherence via Inhibiting Cx43/PKC- α/NOX2/ROS Signaling Pathway in Monocytes. [Abstract]2020 Jul 19;2020:2930463. PMID: 32774667 -
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Immunohorizons
2019 Oct 18;3(10):488-497. PMID: 31628159 -
Oxid Med Cell Longev
Cyclophilin A Protects Cardiomyocytes against Hypoxia/Reoxygenation-Induced Apoptosis via the AKT/Nox2 Pathway. [Abstract]2019 Apr 28:2019:2717986. PMID: 31182989
Solvent & Solubility
DMSO : 100 mg/mL (221.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.55 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Hirano K, et al. Discovery of GSK2795039, a Novel Small Molecule NADPH Oxidase 2 Inhibitor. Antioxid Redox Signal. 2015 Aug 10;23(5):358-74. [Content Brief]
[2]. Yauger YJ, et al. Iron accentuated reactive oxygen species release by NADPH oxidase in activated microglia contributes to oxidative stress in vitro. J Neuroinflammation. 2019 Feb 18;16(1):41. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2195 mL | 11.0973 mL | 22.1946 mL | 55.4865 mL |
| 5 mM | 0.4439 mL | 2.2195 mL | 4.4389 mL | 11.0973 mL | |
| 10 mM | 0.2219 mL | 1.1097 mL | 2.2195 mL | 5.5487 mL | |
| 15 mM | 0.1480 mL | 0.7398 mL | 1.4796 mL | 3.6991 mL | |
| 20 mM | 0.1110 mL | 0.5549 mL | 1.1097 mL | 2.7743 mL | |
| 25 mM | 0.0888 mL | 0.4439 mL | 0.8878 mL | 2.2195 mL | |
| 30 mM | 0.0740 mL | 0.3699 mL | 0.7398 mL | 1.8496 mL | |
| 40 mM | 0.0555 mL | 0.2774 mL | 0.5549 mL | 1.3872 mL | |
| 50 mM | 0.0444 mL | 0.2219 mL | 0.4439 mL | 1.1097 mL | |
| 60 mM | 0.0370 mL | 0.1850 mL | 0.3699 mL | 0.9248 mL | |
| 80 mM | 0.0277 mL | 0.1387 mL | 0.2774 mL | 0.6936 mL | |
| 100 mM | 0.0222 mL | 0.1110 mL | 0.2219 mL | 0.5549 mL |
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