ISRIB (trans-isomer)

Name: ISRIB (trans-isomer)
Brand: MedChemExpress
SKU: HY-12495
Rating: 5.0 (46 reviews)

製品番号: HY-12495 純度: 99.49%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

ISRIB (trans-isomer) is a potent inhibitor of PERK with an IC₅₀ of 5 nM. ISRIB potently reverses the effects of eIF2α phosphorylation (IC₅₀=5 nM).

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 1597403-47-8

容量	価格（税別）	在庫状況	数量

>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 91	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 91	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$83	在庫あり	Estimated Time of Arrival: December 31
10 mg	$130	在庫あり	Estimated Time of Arrival: December 31
25 mg	$254	在庫あり	Estimated Time of Arrival: December 31
50 mg	$432	在庫あり	Estimated Time of Arrival: December 31
100 mg	$780	在庫あり	Estimated Time of Arrival: December 31
250 mg	$1240	在庫あり	Estimated Time of Arrival: December 31
500 mg		お問い合わせ
1 g		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 46 publication(s) in Google Scholar

Other Forms of ISRIB (trans-isomer):

ISRIB 在庫あり

顧客検証

RT-PCR

Cell Imaging/Staining

Bio/Physico-chemical Assay

: ISRIB (trans-isomer) purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Dec 17;10(1):413. [Abstract]; Cells were cotreated with ISRIB (5 μM) and DdBIC to determine the mRNA levels of GZMB.

: ISRIB (trans-isomer) purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Apr 21.; ISRIB (200 nM; 8-72h). The fluorescence intensity of GFP protein in BMDCs, DC2.4, and RAW264.7 were analyzed. Student's t-test, compared to mRNA only group.

: ISRIB (trans-isomer) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Jul;10(20):e2205949. [Abstract]; RT-qPCR analysis of miR‐19a expression in IDHmt U87 cells treated with ISRIB (0-20 μM) in a concentration gradient for 24 h. RNU6B is used as an internal control.

: ISRIB (trans-isomer) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Jul;10(20):e2205949. [Abstract]; ISRIB (10 μM; 48 h). Representative images of BODIPY staining in BV2 cells cultured for 48 h in five types of medium.

: ISRIB (trans-isomer) purchased from MedChemExpress. Usage Cited in: Cell. 2022 Sep 1;185(18):3356-3374.e22. [Abstract]; Double-luciferase assay of WT or BAX, BAK, BOK TKO PC9 cells expressing ATF4 translation reporter and treated with ISRIB 100 μM overnight.

: ISRIB (trans-isomer) purchased from MedChemExpress. Usage Cited in: Oncogene. 2017 Nov 30;36(48):6738-6746. [Abstract]; ISRIB reverses the expression of β-catenin. HCT116 cells are transfected and CT26.WT cells are infected for 48 and 72 h, respectively. The cells are treated with ISRIB (100 nM) for 24 h.

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

生物活性
プロトコル
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

ISRIB (trans-isomer) is a potent inhibitor of PERK with an IC₅₀ of 5 nM. ISRIB potently reverses the effects of eIF2α phosphorylation (IC₅₀=5 nM).

IC₅₀ & Target^[1]

PERK

5 nM (IC₅₀)

体外実験

Trans-ISRIB is 100-fold more potent (IC₅₀=5 nM) than cis-ISRIB (IC₅₀= 600 nM), indicating that the compound’s interaction with its cellular target is stereospecific. ISRIB reduces the viability of cells subjected to PERK-activation by chronic endoplasmic reticulum stress^[1]. ISRIB substantially reverses the translational effects elicited by phosphorylation of eIF2α and induces no major changes in translation or mRNA levels in unstressed cells. eIF2α phosphorylation-induced stress granule (SG) formation is blocked by ISRIB^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

ISRIB increases long-term memory in rodents. ISRIB-treated mice display significant enhancement in spatial and fear-associated learning. ISRIB displays a half-life in plasma of 8 hr and readily crossed the blood-brain barrier, quickly equilibrating with the central nervous system^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

451.34

分子式

C₂₂H₂₄Cl₂N₂O₄

CAS 番号

1597403-47-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(N[C@H]1CC[C@H](NC(COC2=CC=C(Cl)C=C2)=O)CC1)COC3=CC=C(Cl)C=C3

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 4.55 mg/mL (10.08 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2156 mL	11.0781 mL	22.1562 mL
5 mM	0.4431 mL	2.2156 mL	4.4312 mL
10 mM	0.2216 mL	1.1078 mL	2.2156 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 0.83 mg/mL (1.84 mM); Suspended solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

純度とドキュメンテーション

純度: 99.49%

Select Batch:

データシート (272 KB) SDS (392 KB)

COA (243 KB) RP-HPLC (302 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Sidrauski C, et al. Pharmacological brake-release of mRNA translation enhances cognitive memory. Elife. 2013 May 28;2:e00498. [Content Brief]

[2]. Sidrauski C, et al. The small molecule ISRIB reverses the effects of eIF2α phosphorylation on translation and stressgranule assembly. Elife. 2015 Feb 26;4. doi: 10.7554/eLife.05033. [Content Brief]

細胞実験 ^[1]	U2OS cells are plated on 96-well plates and left to recover overnight. Cells are treated with either with 2 µg/ml tunicamycin or 100 nM thapsigargin in the presence or absence of 100 nM ISRIB or with ISRIB alone for the indicated and the level of eIF2α phosphorylation is determined^[1]. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
動物実験 ^[1]	Mice: Intra-peritoneal (ip) route of administration is performed on 6-7 wk old female CD-1 mice. Animals receives a single, 5 mg/kg dose in groups of three mice/compound/route of administration. ISRIB is dissolved in DMSO then diluted 1:1 in Super-Refined PEG 400. Blood (80 μL) is collected from the saphenous vein at intervals post-dosing (20 min, 1 hr, 3 hr, 8 hr, 24 hr) in EDTA containing collection tubes and plasma is prepared for analysis. Compounds are detected by time-of-flight mass spectroscopy^[1]. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
参考文献	[1]. Sidrauski C, et al. Pharmacological brake-release of mRNA translation enhances cognitive memory. Elife. 2013 May 28;2:e00498. [Content Brief] [2]. Sidrauski C, et al. The small molecule ISRIB reverses the effects of eIF2α phosphorylation on translation and stressgranule assembly. Elife. 2015 Feb 26;4. doi: 10.7554/eLife.05033. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2156 mL	11.0781 mL	22.1562 mL	55.3906 mL
	5 mM	0.4431 mL	2.2156 mL	4.4312 mL	11.0781 mL
	10 mM	0.2216 mL	1.1078 mL	2.2156 mL	5.5391 mL