2. Epigenetics Stem Cell/Wnt Protein Tyrosine Kinase/RTK JAK/STAT Signaling
  3. JAK
  4. JAK2 JH2 Tracer

JAK2 JH2 Tracer (Tracer 5) is a fluorescent probe for JAK2 JH2 domain, with a Kd value of 0.2 μM.

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JAK2 JH2 Tracer

JAK2 JH2 Tracer 화학구조

CAS No. : 2101955-00-2

사이즈 가격 재고 수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
1 mg 해외재고보유
5 mg 해외재고보유
10 mg 해외재고보유
50 mg 해외재고보유
100 mg   견적 받기  
200 mg   견적 받기  

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 6 publication(s) in Google Scholar

JAK2 JH2 Tracer Related Antibodies

Top Publications Citing Use of Products

View All JAK Isoform Specific Products:

View All Isoforms
JAK1 JAK2 JAK3 Tyk2 JAK

  • Biological Activity

  • 순도&문서

  • References

  • 고객리뷰

제품 설명

JAK2 JH2 Tracer (Tracer 5) is a fluorescent probe for JAK2 JH2 domain, with a Kd value of 0.2 μM[1][2].

In Vitro

Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
1. Preparation of working solution
1) Preparation of storage solution
Prepare a 10 mM JAK2 JH2 Tracer stock solution using anhydrous DMSO.
2) Preparation of working solution
Dilute the stored solution with pre-heated serum-free cell culture medium or PBS to prepare a 1.5 pM JAK2 JH2 Tracer working solution.
Note: Please adjust the concentration of JAK2 JH2 Tracer working solution according to the actual situation, and prepare it as needed.
2. Specific staining steps
1) Prepare a series of JAK2 JH2 protein solutions with concentrations ranging from 0 to 4.8 μM in the detection buffer.
2) Add 1.5 pM of JAK2 JH2 Tracer working solution to each well of the microplate.
3) Gradually add the JAK2 JH2 protein solution to the wells containing JAK2 JH2 Tracer, ensuring that the final protein concentration covers the required range.
4) Measure the fluorescence polarization value within 90 minutes.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

JAK2 JH2 Tracer Related Antibodies
In Vivo

The possibility that mutations within the JAK2 JH2 domain may be associated with pathology, which is associated with a hyperactive kinase and a hematopoietic malignancy phenotype in flies[2].
Specific mutation in the JAK2 JH2 domain is associated with several myeloproliferative diseases[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
분자량

747.73

화학식

C38H27F2N7O6S
CAS No.
Appearance

Solid

Color

Light brown to brown

Emission (Em)

515
Excitation (Ex)

488
SMILES

S=C(NC1=CC2=C(C3(C4=C(OC5=C3C=CC(O)=C5)C=C(O)C=C4)OC2=O)C=C1)N(CC6=CC=C(NC7=NN(C(C8=C(F)C=CC=C8F)=O)C(N)=N7)C=C6)C
선적

Room temperature in continental US; may vary elsewhere.
보관

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

용액&용해도
In Vitro: 

DMSO : 100 mg/mL (133.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3374 mL 6.6869 mL 13.3738 mL
5 mM 0.2675 mL 1.3374 mL 2.6748 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • 몰농도 계산기

  • 농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
순도&문서
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3374 mL 6.6869 mL 13.3738 mL 33.4345 mL
5 mM 0.2675 mL 1.3374 mL 2.6748 mL 6.6869 mL
10 mM 0.1337 mL 0.6687 mL 1.3374 mL 3.3435 mL
15 mM 0.0892 mL 0.4458 mL 0.8916 mL 2.2290 mL
20 mM 0.0669 mL 0.3343 mL 0.6687 mL 1.6717 mL
25 mM 0.0535 mL 0.2675 mL 0.5350 mL 1.3374 mL
30 mM 0.0446 mL 0.2229 mL 0.4458 mL 1.1145 mL
40 mM 0.0334 mL 0.1672 mL 0.3343 mL 0.8359 mL
50 mM 0.0267 mL 0.1337 mL 0.2675 mL 0.6687 mL
60 mM 0.0223 mL 0.1114 mL 0.2229 mL 0.5572 mL
80 mM 0.0167 mL 0.0836 mL 0.1672 mL 0.4179 mL
100 mM 0.0134 mL 0.0669 mL 0.1337 mL 0.3343 mL
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JAK2 JH2 Tracer Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JAK2 JH2 Tracer2101955-00-2JAKJanus kinaseJNJ7706621NVP-BSK805filgotinibGLPG0634Inhibitorinhibitorinhibit

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상품명:
JAK2 JH2 Tracer
Cat. No.:
HY-102055
수량:
MCE Japan Authorized Agent:

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