1. Immunology/Inflammation
    Metabolic Enzyme/Protease
  2. Aryl Hydrocarbon Receptor
    Endogenous Metabolite
  3. L-Kynurenine

L-Kynurenine 

Cat. No.: HY-104026 Purity: 99.40%
Handling Instructions

L-Kynurenine is a metabolite of the amino acid L-tryptophan. L-Kynurenine is an aryl hydrocarbon receptor agonist.

For research use only. We do not sell to patients.

L-Kynurenine Chemical Structure

L-Kynurenine Chemical Structure

CAS No. : 2922-83-0

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
50 mg USD 50 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products

Publications Citing Use of MCE L-Kynurenine

    L-Kynurenine purchased from MCE. Usage Cited in: PLoS Pathog. 2020 Jul 17;16(7):e1008664.

    MT-1 cells are treated with Kynurenine (10, 50 μM) for 48 h or 4 days , then subjected to analyze the level of cytosolic AHR and nuclear AHR by immunoblot. After 4-days treatment, Kynurenine (10, 50 μM) remarkably induces the expression of CYP1A1 and plus-strand genes in a dose-dependent manner. Both groups efficiently triggered the production of Tax protein, p19 and p24, although the accumulation of gp46 is no apparent.
    • Biological Activity

    • Protocol

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    • References

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    Description

    L-Kynurenine is a metabolite of the amino acid L-tryptophan. L-Kynurenine is an aryl hydrocarbon receptor agonist.

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Kynurenine and its further breakdown products carry out diverse biological functions, including dilating blood vessels during inflammation and regulating the immune response. Some cancers increase kynurenine production, which increases tumor growth. L-kynurenine (Kyn) is an aryl hydrocarbon receptor (AHR) agonist that activates AHR-directed, naive T cell polarization to the anti-inflammatory Treg phenotype. Kynurenine activates AHR signaling at physiological concentrations in H1L7.5c3 cells and acts as an AHR agonist after a 24-hr exposure by inducing the AHR-regulated luciferase gene in H1L7.5c3 mouse hepatocyte cells[1].

    In Vivo

    Kynurenine dilates arteries from rats as well as humans via Kv7 channels in the vascular smooth muscle. In rats, this tryptophan metabolite causes hypotension, which is partly counteracted by Kv7 channel inhibition[2]. L-kynurenine administered 1 h before the hypoxia-ischemia shows a dose-dependent significant neuroprotective effect, with complete protection at a dose of 300 mg/kg. The induction of c-fos immunoreactivity in cerebral cortex is also blocked by this dose of L-kynurenine[3].

    Clinical Trial
    Molecular Weight

    208.21

    Formula

    C₁₀H₁₂N₂O₃

    CAS No.

    2922-83-0

    SMILES

    O=C(O)[[email protected]@H](N)CC(C1=CC=CC=C1N)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (480.28 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.8028 mL 24.0142 mL 48.0284 mL
    5 mM 0.9606 mL 4.8028 mL 9.6057 mL
    10 mM 0.4803 mL 2.4014 mL 4.8028 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (12.01 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (12.01 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: 2.5 mg/mL (12.01 mM); Clear solution; Need warming

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [1]

    Luciferase assays are carried out using the H1L7.5c3 cells. At the indicated times (0.5, 2, 4, 6, 12, 18, 24 h) and concentrations (0.1, 1, 10, 100 μM) of exposures to Kynurenine, cells are removed from incubation and allowed to equilibrate to room temperature for 15min. After equilibration, the medium is removed and the cells are washed twice with at room temperature with DPBS. The cells are lysed with 20 µL/well 1× Passive Lysis Buffer and shaken for 20min at room temperature. Luciferase activity is recorded using an Luminometer Microplate Reader[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [3]

    Rats[3]

    The effects of increasing doses of L-kynurenine with or without probenecid on concentrations of kynurenic acid in cerebral cortex are examined in 7-day-old rats. Six animals are examined in each group. Animals are treated with L-kynurenine at doses of 100, 200, 300, and 400 mg/kg or kynurenine, 200 mg/kg with probenecid, 50 mg/kg. Animals are killed at 1 h, the brains promptly removed, and the cerebral cortex is dissected and placed in 0.5 mL of chilled 0. 1 M HCl. Kynurenic acid measurements are made by high-performance liquid chromatography with fluorescence detection. Protein measurements are made using a fluorometric assay[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 99.40%

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    Keywords:

    L-KynurenineAryl Hydrocarbon ReceptorEndogenous MetaboliteAhRInhibitorinhibitorinhibit

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    Product Name:
    L-Kynurenine
    Cat. No.:
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