LDN193189 Tetrahydrochloride
Based on 93 publication(s) in Google Scholar
LDN193189 (DM-3189) Tetrahydrochloride is a potent selective BMP type I receptor (BMP I) inhibitor. LDN193189 efficiently inhibits transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC50 values of 5 nM and 30 nM, respectively. LDN193189 can be used for the research of bone morphogenetic protein signalling, such as fibrodysplasia ossificans progressiva.
For research use only. We do not sell to patients.
- Purity: 99.79%
- CAS No.: 2310134-98-4
- Formula: C25H26Cl4N6
- Molecular Weight:552.33
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)
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Publications Citing Use of MedChemExpress (MCE) LDN193189 Tetrahydrochloride
More- Nature. 2022 May;605(7909):325-331. [Abstract]
- Cancer Cell. 2026 May 21:S1535-6108(26)00220-5.
- Eur Respir J. 2022 Jul 7;60(1):2100327. [Abstract]
- Mol Cell. 2026 Jun 12:S1097-2765(26)00344-8. [Abstract]
- Mol Cell. 2022 Aug 4;82(15):2779-2796.e10. [Abstract]
- Adv Sci (Weinh). 2025 Nov 7:e12325. [Abstract]
- Adv Sci (Weinh). 2025 Jun 24:e02774. [Abstract]
- Adv Sci (Weinh). 2024 Mar;11(10):e2308072. [Abstract]
- Nucleic Acids Res. 2025 Oct 14;53(19):gkaf995. [Abstract]
- Biomaterials. 2020 May;240:119849. [Abstract]
- Sci Adv. 2025 Nov 28;11(48):eady7682. [Abstract]
- J Hazard Mater. 2024 Mar 5:465:133028. [Abstract]
- Cancer Lett. 2020 Jan 28;469:390-398. [Abstract]
- Cell Commun Signal. 2024 May 30;22(1):300. [Abstract]
- Free Radic Biol Med. 2024 Dec 9:227:296-311. [Abstract]
- Diabetes. 2020 Apr;69(4):634-646. [Abstract]
- Stem Cell Res Ther. 2025 May 13;16(1):239. [Abstract]
- Stem Cell Res Ther. 2024 Dec 18;15(1):478. [Abstract]
- Stem Cell Res Ther. 2023 Jan 5;14(1):1. [Abstract]
- Stem Cell Res Ther. 2022 Sep 2;13(1):436. [Abstract]
- Stem Cell Res Ther. 2022 Aug 19;13(1):424. [Abstract]
- Stem Cell Res Ther. 2021 Jun 11;12(1):345. [Abstract]
- Cell Rep. 2023 Dec 5;42(12):113541. [Abstract]
- Cell Rep. 2017 Aug 29;20(9):2227-2237. [Abstract]
- Elife. 2025 Aug 28:14:RP104774. [Abstract]
- EMBO Rep. 2023 Sep 6;24(9):e56454. [Abstract]
- Antioxid Redox Signal. 2025 Nov;43(13-15):709-726. [Abstract]
- Biomater Adv. 2026 Jun 16:188:215018. [Abstract]
- Neurosci Bull. 2026 Apr 11. [Abstract]
- Neurobiol Dis. 2026 Jun 15:224:107397. [Abstract]
- Mar Drugs. 2022 Nov 14;20(11):715. [Abstract]
- Cancer Metab. 2023 Jul 13;11(1):9. [Abstract]
- Cells. 2026 Jun 17;15(12):1101. [Abstract]
- Int J Mol Sci. 2024 Apr 9;25(8):4138. [Abstract]
- Int J Mol Sci. 2022 Nov 27;23(23):14831. [Abstract]
- Cell Regen. 2025 May 16;14(1):18. [Abstract]
- Cell Rep Methods. 2026 Apr 20;6(4):101372. [Abstract]
- Cell Rep Methods. 2024 May 8:100777. [Abstract]
- Front Cell Dev Biol. 2021 May 13:9:673231. [Abstract]
- FASEB J. 2026 Apr 15;40(7):e71420. [Abstract]
- iScience. 2024 Feb 5;27(3):109111. [Abstract]
- iScience. 2022 Dec 26;26(1):105898. [Abstract]
- Transl Oncol. 2019 Dec 19;13(2):125-134 [Abstract]
- iScience. 2019 Sep 27;19:1248-1259. [Abstract]
- Front Mol Biosci. 2021 Apr 26:8:663987. [Abstract]
- Oncol Rep. 2026 Apr;55(4):62. [Abstract]
- J Biomed Mater Res A. 2025 Oct;113(10):e38001. [Abstract]
- Biomedicines. 2022 Apr 18;10(4):928. [Abstract]
- Sci Rep. 2022 Mar 25;12(1):5221. [Abstract]
- Sci Rep. 2022 Mar 18;12(1):4740. [Abstract]
- Cell Signal. 2025 Sep 8:136:112116. [Abstract]
- Cardiovasc Toxicol. 2019 Jun;19(3):264-275. [Abstract]
- Stem Cells. 2023 Jun 15;41(6):603-616. [Abstract]
- Stem Cells Int. 2022 Dec 22:2022:1278921. [Abstract]
- Stem Cells Int. 2022 Apr 28;2022:1560943. [Abstract]
- Cell Transplant. 2024 Jan-Dec:33:9636897241288932. [Abstract]
- Neuroscience. 2026 Jun 6:610:122-135. [Abstract]
- Pigment Cell Melanoma Res. 2024 Jan;37(1):21-35. [Abstract]
- Prostate. 2021 Dec;81(16):1435-1449. [Abstract]
- Int J Stem Cells. 2023 May 30;16(2):156-167. [Abstract]
- J Oral Biosci. 2026 Feb;68(1):100735. [Abstract]
- Exp Ther Med. 2019 Apr;17(4):2648-2656. [Abstract]
- J Recept Signal Transduct Res. 2017 Oct;37(5):515-521. [Abstract]
- Biochim Biophys Acta Gen Subj. 2022 Jan;1866(1):130046. [Abstract]
- Int J Ophthalmol. 2022 May 18;15(5):711-720. [Abstract]
- STAR Protoc. 2024 Nov 27;5(4):103466. [Abstract]
- Dev Growth Differ. 2025 Apr;67(3):149-164. [Abstract]
- bioRxiv. 2026 Apr 24.
- Northwestern University. 2026.
- Animal Research and One Health. 2026 Mar 19.
- bioRxiv. 2026 Feb 16:2026.02.13.705790. [Abstract]
- Res Sq. 2026 Jan 20:rs.3.rs-8607320. [Abstract]
- bioRxiv. 2025 Oct 11.
- bioRxiv. 2025 Aug 8:2025.08.08.669417. [Abstract]
- bioRxiv. 2025 April 14.
- bioRxiv. 2025 February 21.
- bioRxiv. 2025 March 15.
- Res Sq. 2024 Nov 14.
- bioRxiv. 2024 November 15.
- bioRxiv. 2024 November 28.
- bioRxiv. 2024 Nov 4:2024.10.08.617155. [Abstract]
- Patent. US20240247230A1.
- bioRxiv. 2024 Aug 6:2024.08.05.606734. [Abstract]
- bioRxiv. 2024 Jul 11:2024.07.08.602611. [Abstract]
- bioRxiv. 2024 July 03.
- bioRxiv. 2024 Mar 13.
- bioRxiv. 2024 Mar 25:2024.03.21.585753. [Abstract]
- Research Square Preprint. 2023 May 17.
- Patent. US20210346386A1.
- Research Square Preprint. 2021 Sep.
- Oxid Med Cell Longev. 2020 Jun 8;2020:4175613. [Abstract]
- Curr Protoc Cell Biol. 2018 Dec;81(1):e62. [Abstract]
- Patent. US20180263995A1.
Customer Validation & Images
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2D/3D Cell Culture and Differentiation
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Biological Activity
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ACVR1 5 nM (IC50) |
BMPR1A 30 nM (IC50) |
LDN193189 Tetrahydrochloride efficiently inhibits transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC50 values of 5 nM and 30 nM, respectively[1].
LDN193189 Tetrahydrochloride has weake effects on activin and the TGF-β type I receptors ALK4, ALK5 and ALK7 with IC50 values of ≥ 500 nM[1].
LDN193189 Tetrahydrochloride binds ActRIIA with Kd value of 14 nM[2].
LDN193189 Tetrahydrochloride (0.5 μM; 30 min) targets GDF8 induced Smad2/3 signaling and repression of myogenic transcription factors[2].
LDN193189 Tetrahydrochloride (0.05, 0.5, 5 μM) efficiently inhibits GDF8 induced Smad3/4 reporter gene activity[2].
LDN193189 Tetrahydrochloride (0-5 μM) rescues myogenesis in myoblasts treated with GDF8[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
LDN193189 (i.p.; 3 mg/kg; single) Tetrahydrochloride shows a reduction in ectopic ossification and functional impairment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2310134-98-4
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Appearance Solid
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Molecular Weight 552.33
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Formula C25H26Cl4N6
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Color Light yellow to yellow
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SMILES
C1(C2=C3N=CC(C4=CC=C(N5CCNCC5)C=C4)=CN3N=C2)=CC=NC6=CC=CC=C16.Cl.Cl.Cl.Cl
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Synonyms
DM-3189 Tetrahydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)
Publications (93)
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Journal Impact Factor
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Most Recent
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Nature
2022 May;605(7909):325-331. PMID: 35418683 -
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Eur Respir J
Single-cell RNA sequencing reveals that BMPR2 mutation regulates right ventricular function via ID genes. [Abstract]2022 Jul 7;60(1):2100327. PMID: 34857612 -
Mol Cell
Distinct associations of pioneer factor Ascl1-E12a with nucleosomes drive changes in cell fate. [Abstract]2026 Jun 12:S1097-2765(26)00344-8. PMID: 42285106 -
Mol Cell
AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay. [Abstract]2022 Aug 4;82(15):2779-2796.e10. PMID: 35675814 -
Adv Sci (Weinh)
Quantifying the Single-Cell Morphological Landscape of Cellular Transdifferentiation through Force Field Reconstruction. [Abstract]2025 Nov 7:e12325. PMID: 41203584 -
Adv Sci (Weinh)
Single-Cell RNA Sequencing Identifies MMP11+ Cancer-Associated Fibroblasts as Drivers of Angiogenesis and Bladder Cancer Progression. [Abstract]2025 Jun 24:e02774. PMID: 40552583 -
Adv Sci (Weinh)
2024 Mar;11(10):e2308072. PMID: 38239021 -
Nucleic Acids Res
mRNA turnover dynamics are affected by cell differentiation and loss of the cytosine methyltransferase Nsun2. [Abstract]2025 Oct 14;53(19):gkaf995. PMID: 41099711 -
Biomaterials
2020 May;240:119849. PMID: 32087458 -
Sci Adv
Specialized signaling centers direct cell fate and spatial organization in a mesodermal organoid model. [Abstract]2025 Nov 28;11(48):eady7682. PMID: 41313763
LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Nov 28;11(48):eady7682. [Abstract]
The bar chart displayed the weighted average of day 13 budoid proportions exhibiting no elongation, elongation (short), or elongation (long) after treatment with LDN193189 Tetrahydrochloride (1-4 μM).
LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Nov 28;11(48):eady7682. [Abstract]
Max-projection confocal images of day 13 budoids were treated with LDN193189 Tetrahydrochloride (LDN) (1 μM). Gray: Hoechst; magenta: SOX9.
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J Hazard Mater
Typical neonicotinoids and organophosphate esters, but not their metabolites, adversely impact early human development by activating BMP4 signaling. [Abstract]2024 Mar 5:465:133028. PMID: 38006857 -
Cancer Lett
2020 Jan 28;469:390-398. PMID: 31730904
LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2020 Jan 28;469:390-398. [Abstract]
LDN193189 Tetrahydrochloride (0.2–0.3 μM; 24 h) blocked Smad1/5/9 phosphorylation in KYSE30 and KYSE150 cells.
LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2020 Jan 28;469:390-398. [Abstract]
LDN193189 Tetrahydrochloride (LDN) (0.2–0.3 μM; 24 h) inhibited the migration of KYSE30 and KYSE150 cells.
LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2020 Jan 28;469:390-398. [Abstract]
Transwell assays were performed on SERPINE2-overexpressing KYSE30 and KYSE150 cells treated with or without LDN193189 Tetrahydrochloride (0.2–0.3 μM; 24 h) (LDN) in the lower chamber.
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Cell Commun Signal
Low concentrations of saracatinib promote definitive endoderm differentiation through inhibition of FAK-YAP signaling axis. [Abstract]2024 May 30;22(1):300. PMID: 38816763 -
Free Radic Biol Med
2024 Dec 9:227:296-311. PMID: 39653130 -
Diabetes
Insulin-Deficient Diabetic Condition Upregulates the Insulin-Secreting Capacity of Human Induced Pluripotent Stem Cell-Derived Pancreatic Endocrine Progenitor Cells After Implantation in Mice. [Abstract]2020 Apr;69(4):634-646. PMID: 32005704 -
Stem Cell Res Ther
Mesenchymal stem cells derived from hPSC via neural crest attenuate chemotherapy-induced premature ovarian insufficiency by ameliorating apoptosis and oxidative stress in granulosa cells. [Abstract]2025 May 13;16(1):239. PMID: 40361250 -
Stem Cell Res Ther
Transcriptomic and proteomic sequencing unveils the role of vitamin D and metabolic flux shifts in the induction of human hepatic organoids. [Abstract]2024 Dec 18;15(1):478. PMID: 39696644 -
Stem Cell Res Ther
CDK8/19 inhibition plays an important role in pancreatic β-cell induction from human iPSCs. [Abstract]2023 Jan 5;14(1):1. PMID: 36600289 -
Stem Cell Res Ther
Stable expression of a truncated TLX variant drives differentiation of induced pluripotent stem cells into self-renewing neural stem cells for production of extracellular vesicles. [Abstract]2022 Sep 2;13(1):436. PMID: 36056423 -
Stem Cell Res Ther
Aucubin promotes bone-fracture healing via the dual effects of anti-oxidative damage and enhancing osteoblastogenesis of hBM-MSCs. [Abstract]2022 Aug 19;13(1):424. PMID: 35986345
LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2022 Aug 19;13(1):424. [Abstract]
Phospho-Smad1/5/9, COL1A1 and RUNX2 were quantified in hBM-MSCs after 3 days of treatment with LDN193189 (100 nM; 3 days) by western blot analysis and normalized to GAPDH and tSmad1.
LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2022 Aug 19;13(1):424. [Abstract]
LDN193189 (100 nM; 3 days). Representative images and quantification of immunofluorescence staining of COL1A1. Scale bars, 100 μm.
LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2022 Aug 19;13(1):424. [Abstract]
LDN193189 (100 nM; 3 days). Representative images of ARS.
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Stem Cell Res Ther
Photoactivation of TGFβ/SMAD signaling pathway ameliorates adult hippocampal neurogenesis in Alzheimer's disease model. [Abstract]2021 Jun 11;12(1):345. PMID: 34116709 -
Cell Rep
ASCL1 characterizes adrenergic neuroblastoma via its pioneer function and cooperation with core regulatory circuit factors. [Abstract]2023 Dec 5;42(12):113541. PMID: 38060444 -
Cell Rep
Generation of Mouse Haploid Somatic Cells by Small Molecules for Genome-wide Genetic Screening. [Abstract]2017 Aug 29;20(9):2227-2237. PMID: 28854370 -
Elife
Enalapril mitigates senescence and aging-related phenotypes in human cells and mice via pSmad1/5/9-driven antioxidative genes. [Abstract]2025 Aug 28:14:RP104774. PMID: 40874922 -
EMBO Rep
Terminal differentiation of villus tip enterocytes is governed by distinct Tgfβ superfamily members. [Abstract]2023 Sep 6;24(9):e56454. PMID: 37493498 -
Antioxid Redox Signal
Remifentanil Mitigates Hepatic Ischemia/Reperfusion-Induced D1-Medium Spiny Neurons Damage via Fibroblast Growth Factor 18 Upregulation. [Abstract]2025 Nov;43(13-15):709-726. PMID: 40810178 -
Biomater Adv
A supramolecular MgTA@MnO₂ nanozyme platform supports human spinal cord organoid structural integration and locomotor recovery via microenvironmental reprogramming. [Abstract]2026 Jun 16:188:215018. PMID: 42309040 -
Neurosci Bull
Engineered Alzheimer Organoids Validate the Link Between Intracellular and Soluble p-Tau Biomarkers and Highlight the Contribution of Astrocytic Tau. [Abstract]2026 Apr 11. PMID: 41964788 -
Neurobiol Dis
Neuronal ACVR1-mediated H3K18 lactylation drives NLRP3 pyroptosis to sustain neuropathic pain via metabolic-epigenetic coupling. [Abstract]2026 Jun 15:224:107397. PMID: 41991084 -
Mar Drugs
2022 Nov 14;20(11):715. PMID: 36421992 -
Cancer Metab
BMP4 upregulates glycogen synthesis through the SMAD/SLC2A1 (GLUT1) signaling axis in hepatocellular carcinoma (HCC) cells. [Abstract]2023 Jul 13;11(1):9. PMID: 37443106 -
Cells
Compensatory Intercellular Mitochondrial Transfer Improves Bioenergetics in P301L Tau-Affected Neuronal Cells. [Abstract]2026 Jun 17;15(12):1101. PMID: 42346128 -
Int J Mol Sci
Small Molecules Promote the Rapid Generation of Dental Epithelial Cells from Human-Induced Pluripotent Stem Cells. [Abstract]2024 Apr 9;25(8):4138. PMID: 38673725 -
Int J Mol Sci
BMP/Smad Pathway Is Involved in Lithium Carbonate-Induced Neural-Tube Defects in Mice and Neural Stem Cells. [Abstract]2022 Nov 27;23(23):14831. PMID: 36499158 -
Cell Regen
The critical role of BMP signaling in gastric epithelial cell differentiation revealed by organoids. [Abstract]2025 May 16;14(1):18. PMID: 40377813 -
Cell Rep Methods
Wnt activation and dual SMAD inhibition for induction and maintenance of hindbrain-like neural stem cell from hiPSCs. [Abstract]2026 Apr 20;6(4):101372. PMID: 41916305 -
Cell Rep Methods
2024 May 8:100777. PMID: 38744289 -
Front Cell Dev Biol
miR-212/132-Enriched Extracellular Vesicles Promote Differentiation of Induced Pluripotent Stem Cells Into Pancreatic Beta Cells. [Abstract]2021 May 13:9:673231. PMID: 34055806 -
FASEB J
Transcription Factor GATA3 Ameliorates Sepsis-Associated Acute Respiratory Distress Syndrome by Activating Smad1/5-YAP Pathway via BMP9. [Abstract]2026 Apr 15;40(7):e71420. PMID: 41920072 -
iScience
Modeling the precise interaction of glioblastoma with human brain region-specific organoids. [Abstract]2024 Feb 5;27(3):109111. PMID: 38390494 -
iScience
Generation of dorsoventral human spinal cord organoids via functionalizing composite scaffold for drug testing. [Abstract]2022 Dec 26;26(1):105898. PMID: 36647382 -
Transl Oncol
Autocrine BMP4 Signaling Enhances Tumor Aggressiveness via Promoting Wnt/β-Catenin Signaling in IDH1-mutant Gliomas. [Abstract]2019 Dec 19;13(2):125-134 PMID: 31865175 -
iScience
2019 Sep 27;19:1248-1259. PMID: 31353167 -
Front Mol Biosci
Macrophages Inhibit Ciliary Protein Levels by Secreting BMP-2 Leading to Airway Epithelial Remodeling Under Cigarette Smoke Exposure. [Abstract]2021 Apr 26:8:663987. PMID: 33981724 -
Oncol Rep
POSTN+ fibroblast‑secreted small extracellular vesicles drive macrophage M2 polarization through BMP4/BMPR2/Smad signaling. [Abstract]2026 Apr;55(4):62. PMID: 41645751 -
J Biomed Mater Res A
GelMA Hydrogel Encapsulating iPSC-Derived Human Spinal Cord Organoids Enhances Neural Regeneration and Restores Motor Function in Rat Spinal Cord Injury. [Abstract]2025 Oct;113(10):e38001. PMID: 41051068 -
Biomedicines
Generation of a Pure Culture of Neuron-like Cells with a Glutamatergic Phenotype from Mouse Astrocytes. [Abstract]2022 Apr 18;10(4):928. PMID: 35453678 -
Sci Rep
2022 Mar 25;12(1):5221. PMID: 35338209 -
Sci Rep
Characterization and reduction of non-endocrine cells accompanying islet-like endocrine cells differentiated from human iPSC. [Abstract]2022 Mar 18;12(1):4740. PMID: 35304548 -
Cell Signal
Inhibition of ferroptosis rescues BMP osteogenic differentiation impaired by iron overload in the osteoporotic microenvironment. [Abstract]2025 Sep 8:136:112116. PMID: 40930476 -
Cardiovasc Toxicol
MiR-15b-5p is Involved in Doxorubicin-Induced Cardiotoxicity via Inhibiting Bmpr1a Signal in H9c2 Cardiomyocyte. [Abstract]2019 Jun;19(3):264-275. PMID: 30535663
LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Cardiovasc Toxicol. 2019 Jun;19(3):264-275. [Abstract]
H9c2 cells are treated as following: control, DOX, DOX + rhBMP (Bmpr1a agonist) and DOX + LDN193189 (Bmpr1a inhibitor). Cells with red fluorescence indicate favorable MMP while green fluorescence indicates damage.
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Stem Cells
Neuroprotective effects of genome-edited human iPS cell-derived neural stem/progenitor cells on traumatic brain injury. [Abstract]2023 Jun 15;41(6):603-616. PMID: 37029780 -
Stem Cells Int
2022 Dec 22:2022:1278921. PMID: 36591373 -
Stem Cells Int
Palovarotene Can Attenuate Heterotopic Ossification Induced by Tendon Stem Cells by Downregulating the Synergistic Effects of Smad and NF- κ B Signaling Pathway following Stimulation of the Inflammatory Microenvironment. [Abstract]2022 Apr 28;2022:1560943. PMID: 35530413 -
Cell Transplant
Xenogenic Engraftment of Human-Induced Pluripotent Stem Cell-Derived Pancreatic Islet Cells in an Immunosuppressive Diabetic Göttingen Mini-Pig Model. [Abstract]2024 Jan-Dec:33:9636897241288932. PMID: 39401129 -
Neuroscience
Trichostatin A-primed spinal cord organoids alleviate oxidative stress and improve recovery after spinal cord injury involving the NRF2/HO-1 signaling pathway. [Abstract]2026 Jun 6:610:122-135. PMID: 42250855 -
Pigment Cell Melanoma Res
Modeling of pigmentation disorders associated with MITF mutation in Waardenburg syndrome revealed an impaired melanogenesis pathway in iPS-derived melanocytes. [Abstract]2024 Jan;37(1):21-35. PMID: 37559350 -
Prostate
Upregulated bone morphogenetic protein 5 enhances proliferation and epithelial-mesenchymal transition process in benign prostatic hyperplasia via BMP/Smad signaling pathway. [Abstract]2021 Dec;81(16):1435-1449. PMID: 34553788 -
Int J Stem Cells
Strategic Application of Epigenetic Regulators for Efficient Neuronal Reprogramming of Human Fibroblasts. [Abstract]2023 May 30;16(2):156-167. PMID: 36823979 -
J Oral Biosci
Bone morphogenetic protein-2 and transforming growth factor-β1 regulate cell proliferation and ectopic claudin-4 expression in parotid acinar cells. [Abstract]2026 Feb;68(1):100735. PMID: 41714034 -
Exp Ther Med
Bone morphogenetic protein 4 promotes the differentiation of Tbx18-positive epicardial progenitor cells to pacemaker-like cells. [Abstract]2019 Apr;17(4):2648-2656. PMID: 30906456 -
J Recept Signal Transduct Res
Functional expression of BMP7 receptors in oral epithelial cells. Interleukin-17F production in response to BMP7. [Abstract]2017 Oct;37(5):515-521. PMID: 28812969 -
Biochim Biophys Acta Gen Subj
Expression of GALNT8 and O-glycosylation of BMP receptor 1A suppress breast cancer cell proliferation by upregulating ERα levels. [Abstract]2022 Jan;1866(1):130046. PMID: 34743989 -
Int J Ophthalmol
Chordin-like 2 influences the differentiation fate of retinal pigment epithelium cells by dynamically regulating BMP pathway. [Abstract]2022 May 18;15(5):711-720. PMID: 35601169 -
STAR Protoc
2024 Nov 27;5(4):103466. PMID: 39607824 -
Dev Growth Differ
Transforming growth factor-β-mediated regulation of atoh1-expressing neural progenitors is involved in the generation of cerebellar granule cells in larval and adult zebrafish. [Abstract]2025 Apr;67(3):149-164. PMID: 40012512 -
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bioRxiv
Neuronal Cell-Cycle Re-entry Defines Divergent Outcomes Through Replication-Dependent DNA Damage in ALS. [Abstract]2026 Feb 16:2026.02.13.705790. PMID: 41756973 -
Res Sq
2026 Jan 20:rs.3.rs-8607320. PMID: 41646411 -
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bioRxiv
2025 Aug 8:2025.08.08.669417. PMID: 40855884 -
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bioRxiv
2024 Nov 4:2024.10.08.617155. PMID: 39415993 -
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bioRxiv
Human Vascularized Macrophage-Islet Organoids to Model Immune-Mediated Pancreatic β cell Pyroptosis upon Viral Infection. [Abstract]2024 Aug 6:2024.08.05.606734. PMID: 39149298 -
bioRxiv
2024 Jul 11:2024.07.08.602611. PMID: 39026750 -
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bioRxiv
2024 Mar 25:2024.03.21.585753. PMID: 38585971 -
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Oxid Med Cell Longev
The Attenuating Effect of the Intraovarian Bone Morphogenetic Protein 4 on Age-Related Endoplasmic Reticulum Stress in Chicken Follicular Cells. [Abstract]2020 Jun 8;2020:4175613. PMID: 32587659
LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2020 Jun 8;2020:4175613. [Abstract]
Western blot and grey analysis of CYP11a1, CYP19a1, BCL2, CALR, CHOP, caspase3, caspase12, PERK, and GRP78. qRT-PCR analyzed ATF4, caspase3, and GRP78 mRNAs. The relieving function of BMP4 on ER stress is diminished after blockade of BMP receptor I (BMPRI) with DM-3189.
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Curr Protoc Cell Biol
2018 Dec;81(1):e62. PMID: 30239150 -
Solvent & Solubility
H2O : 33.33 mg/mL (60.34 mM; Need ultrasonic)
DMSO : 4.17 mg/mL (7.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
Mouse PASMCs grown are transiently transfected to 50% confluence in six-well plates with 0.3 μg Id1promoter luciferase reporter construct (BRE-Luc) in combination with 0.6 μg of plasmid expressing constitutively active forms of BMP type I receptors (caALK2, caALK3 or caALK6). For both reporter plasmids, 0.2 μg of pRL-TKRenilla luciferase are used to control for transfection efficiency. PASMCs are incubated with LDN193189 (2 nM-32 μM) or vehicle starting 1 h after transfection. Cell extracts are harvested and quantified relative promoter activity by the ratio of firefly to Renilla luciferase activity with the dual luciferase assay kit[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice[2]
In the first experiment, SCID mice are implanted with MDA-PCa-118b tumors. After 7 days when tumors reached measurable sizes, mice are injected with LDN193189 (3 mg/kg) or with vehicle intraperitoneally twice a day. Tumor sizes and body weights are measured weekly. Mice are injected with calcein at three days and one day prior to sacrifice. Blood is collected and tumors are weighed. A portion of the tumors are fixed in formaldehyde for micro-computed tomography (microCT), using EVS CT, or further decalcified for bone histomorphometric analysis, using OsteoMeasure Analysis System, or flash frozen for RNA preparation. Osteocalcin in the mouse serum is determined by ELISA. In the second experiment, PCa-118b tumors are first digested with Accumax, and the isolated cells are plated overnight, resuspended in Matrigel in 1:1 ratio, and injected into SCID mice (1×106 cells/mouse) subcutaneously. Mice are treated with LDN193189 five days post-injection.
Rats[3]
Male Sprague-Dawley (SD) rats, 8 weeks of age, weighing 200-220 g, are purchased from Nanjing Medical University animal center. Rats are randomly assigned to one of seven experiment groups (n=6 per group). Rats are housed with free access to food and water under a natural 12/12 h day/night cycle. The Monocrotaline is administered (60 mg/kg) to rats by subcutaneous injection into the back region. The animal’s lungs are harvested at 28th day of the study after hemodynamic assessment. The UK-92480 group received daily intragastric administration of UK-92480 after the administration of MCT (60 mg/kg). The LDN193189 group received daily intragastric administration of UK-92480 (50 mg/kg) and intraperitoneal injection of LDN193189 (10 mg/kg). In other groups, the same volume saline is given.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (286 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Yu PB, et al. BMP type I receptor inhibition reduces heterotopic [corrected] ossification. Nat Med, 2008, 14(12), 1363-1369. [Content Brief]
[2]. Daniel Horbelt, et al. Small molecules dorsomorphin and LDN-193189 inhibit myostatin/GDF8 signaling and promote functional myoblast differentiation. J Biol Chem. 2015 Feb 6;290(6):3390-404. [Content Brief]
[3]. Julien Vollaire, et al. The Bone Morphogenetic Protein Signaling Inhibitor LDN-193189 Enhances Metastasis Development in Mice. Front Pharmacol. 2019 Jun 19;10:667. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.8105 mL | 9.0526 mL | 18.1051 mL | 45.2628 mL |
| 5 mM | 0.3621 mL | 1.8105 mL | 3.6210 mL | 9.0526 mL | |
| H2O | 10 mM | 0.1811 mL | 0.9053 mL | 1.8105 mL | 4.5263 mL |
| 15 mM | 0.1207 mL | 0.6035 mL | 1.2070 mL | 3.0175 mL | |
| 20 mM | 0.0905 mL | 0.4526 mL | 0.9053 mL | 2.2631 mL | |
| 25 mM | 0.0724 mL | 0.3621 mL | 0.7242 mL | 1.8105 mL | |
| 30 mM | 0.0604 mL | 0.3018 mL | 0.6035 mL | 1.5088 mL | |
| 40 mM | 0.0453 mL | 0.2263 mL | 0.4526 mL | 1.1316 mL | |
| 50 mM | 0.0362 mL | 0.1811 mL | 0.3621 mL | 0.9053 mL | |
| 60 mM | 0.0302 mL | 0.1509 mL | 0.3018 mL | 0.7544 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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