LDN193189 dihydrochloride (Synonyms: DM-3189 dihydrochloride)

Name: LDN193189 dihydrochloride
Brand: MedChemExpress
SKU: HY-12071B
Rating: 5.0 (83 reviews)

Cat. No.: HY-12071B Purity: 99.50%: Data Sheet
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LDN193189 (DM-3189) dihydrochloride is a potent selective BMP type I receptor (BMP I) inhibitor. LDN193189 efficiently inhibits transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC₅₀ values of 5 nM and 30 nM, respectively. LDN193189 can be used for the research of bone morphogenetic protein signalling, such as fibrodysplasia ossificans progressiva.

For research use only. We do not sell to patients.

CAS No. : 1435934-00-1

Size	Stock	Quantity

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 83 publication(s) in Google Scholar

Other Forms of LDN193189 dihydrochloride:

LDN193189 In-stock
LDN193189 Tetrahydrochloride In-stock
LDN193189 dihydrochloride (Standard) Get quote

83 Publications Citing Use of MCE LDN193189 dihydrochloride

2D/3D Cell Culture and Differentiation

Cell Migration/Invasion Assay

IHC

: LDN193189 dihydrochloride purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Nov 28;11(48):eady7682. [Abstract]; The bar chart displayed the weighted average of day 13 budoid proportions exhibiting no elongation, elongation (short), or elongation (long) after treatment with LDN193189 Tetrahydrochloride (1-4 μM).

: LDN193189 dihydrochloride purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Nov 28;11(48):eady7682. [Abstract]; Max-projection confocal images of day 13 budoids were treated with LDN193189 Tetrahydrochloride (LDN) (1 μM). Gray: Hoechst; magenta: SOX9.

: LDN193189 dihydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2022 Aug 19;13(1):424. [Abstract]; Phospho-Smad1/5/9, COL1A1 and RUNX2 were quantified in hBM-MSCs after 3 days of treatment with LDN193189 (100 nM; 3 days) by western blot analysis and normalized to GAPDH and tSmad1.

: LDN193189 dihydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2022 Aug 19;13(1):424. [Abstract]; LDN193189 (100 nM; 3 days). Representative images and quantification of immunofluorescence staining of COL1A1. Scale bars, 100 μm.

: LDN193189 dihydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2022 Aug 19;13(1):424. [Abstract]; LDN193189 (100 nM; 3 days). Representative images of ARS.

: LDN193189 dihydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2020 Jan 28;469:390-398. [Abstract]; LDN193189 Tetrahydrochloride (0.2–0.3 μM; 24 h) blocked Smad1/5/9 phosphorylation in KYSE30 and KYSE150 cells.

: LDN193189 dihydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2020 Jan 28;469:390-398. [Abstract]; LDN193189 Tetrahydrochloride (LDN) (0.2–0.3 μM; 24 h) inhibited the migration of KYSE30 and KYSE150 cells.

: LDN193189 dihydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2020 Jan 28;469:390-398. [Abstract]; Transwell assays were performed on SERPINE2-overexpressing KYSE30 and KYSE150 cells treated with or without LDN193189 Tetrahydrochloride (0.2–0.3 μM; 24 h) (LDN) in the lower chamber.

: LDN193189 dihydrochloride purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2020 Jun 8;2020:4175613. [Abstract]; Western blot and grey analysis of CYP11a1, CYP19a1, BCL2, CALR, CHOP, caspase3, caspase12, PERK, and GRP78. qRT-PCR analyzed ATF4, caspase3, and GRP78 mRNAs. The relieving function of BMP4 on ER stress is diminished after blockade of BMP receptor I (BMPRI) with DM-3189.

: LDN193189 dihydrochloride purchased from MedChemExpress. Usage Cited in: Cardiovasc Toxicol. 2019 Jun;19(3):264-275. [Abstract]; H9c2 cells are treated as following: control, DOX, DOX + rhBMP (Bmpr1a agonist) and DOX + LDN193189 (Bmpr1a inhibitor). Cells with red fluorescence indicate favorable MMP while green fluorescence indicates damage.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 5 nM (ALK2), 30 nM (ALK3)^[1]

In Vitro

LDN193189 dihydrochloride efficiently inhibits transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC₅₀ values of 5 nM and 30 nM, respectively^[1].
LDN193189 dihydrochloride has weake effects on activin and the TGF-β type I receptors ALK4, ALK5 and ALK7 with IC₅₀ values of ≥ 500 nM^[1].
LDN193189 dihydrochloride binds ActRIIA with K_d value of 14 nM^[2].
LDN193189 dihydrochloride (0.5 μM; 30 min) targets GDF8 induced Smad2/3 signaling and repression of myogenic transcription factors^[2].
LDN193189 dihydrochloride (0.05, 0.5, 5 μM) efficiently inhibits GDF8 induced Smad3/4 reporter gene activity^[2].
LDN193189 dihydrochloride (0-5 μM) rescues myogenesis in myoblasts treated with GDF8^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

LDN193189 (i.p.; 3 mg/kg; daily; for 35 days) dihydrochloride might affect the interaction between breast cancer cells and the bone environment^[3].
LDN193189 (i.p.; 3 mg/kg; single) dihydrochloride shows a reduction in ectopic ossification and functional impairment^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

479.40

Formula

C₂₅H₂₄Cl₂N₆

CAS No.

1435934-00-1

Appearance

Solid

Color

Orange to red

SMILES

C1(C2=C3N=CC(C4=CC=C(N5CCNCC5)C=C4)=CN3N=C2)=CC=NC6=CC=CC=C16.Cl.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 25 mg/mL (52.15 mM; Need ultrasonic)

DMSO : 10 mg/mL (20.86 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0859 mL	10.4297 mL	20.8594 mL
5 mM	0.4172 mL	2.0859 mL	4.1719 mL
10 mM	0.2086 mL	1.0430 mL	2.0859 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.50%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (256 KB) HNMR (146 KB) LCMS (98 KB)

Handling Instructions (2659 KB)

References

[1]. Yu PB, et al. BMP type I receptor inhibition reduces heterotopic [corrected] ossification. Nat Med, 2008, 14(12), 1363-1369. [Content Brief]

[2]. Daniel Horbelt, et al. Small molecules dorsomorphin and LDN-193189 inhibit myostatin/GDF8 signaling and promote functional myoblast differentiation. J Biol Chem. 2015 Feb 6;290(6):3390-404. [Content Brief]

[3]. Julien Vollaire, et al. The Bone Morphogenetic Protein Signaling Inhibitor LDN-193189 Enhances Metastasis Development in Mice. Front Pharmacol. 2019 Jun 19;10:667. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.0859 mL	10.4297 mL	20.8594 mL	52.1485 mL
	5 mM	0.4172 mL	2.0859 mL	4.1719 mL	10.4297 mL
	10 mM	0.2086 mL	1.0430 mL	2.0859 mL	5.2149 mL
	15 mM	0.1391 mL	0.6953 mL	1.3906 mL	3.4766 mL
	20 mM	0.1043 mL	0.5215 mL	1.0430 mL	2.6074 mL
H₂O	25 mM	0.0834 mL	0.4172 mL	0.8344 mL	2.0859 mL
	30 mM	0.0695 mL	0.3477 mL	0.6953 mL	1.7383 mL
	40 mM	0.0521 mL	0.2607 mL	0.5215 mL	1.3037 mL
	50 mM	0.0417 mL	0.2086 mL	0.4172 mL	1.0430 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

LDN193189 dihydrochloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LDN1931891435934-00-1DM-3189LDN 193189LDN-193189DM3189DM 3189OrganoidTGF-β ReceptorTransforming growth factor beta receptorsInhibitorinhibitorinhibit

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LDN193189 dihydrochloride (Synonyms: DM-3189 dihydrochloride)

Customer Review

Other Forms of LDN193189 dihydrochloride:

LDN193189 dihydrochloride Related Antibodies

83 Publications Citing Use of MCE LDN193189 dihydrochloride

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All TGF-β Receptor Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

LDN193189 dihydrochloride Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

LDN193189 dihydrochloride Related Classifications

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