1. Stem Cell/Wnt
  2. Wnt

IWR-1 (Synonyms: endo-IWR 1; IWR-1-endo)

Cat. No.: HY-12238 Purity: 99.83%
Handling Instructions

IWR-1 is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, and induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.

For research use only. We do not sell to patients.

IWR-1 Chemical Structure

IWR-1 Chemical Structure

CAS No. : 1127442-82-3

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
5 mg USD 60 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
10 mg USD 92 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
50 mg USD 414 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

    IWR-1 purchased from MCE. Usage Cited in: Cell Biol Int. 2017 May;41(5):534-543.

    SCAPs are cultured with modified neural differentiation medium for the indicated time periods. The neuron-like cells in control group, SFRP2 over-expressed group and 10 uM IWR1-endo treated group are positive for βIII-tubulin (red) expression by using immunofluorescence staining.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    IWR-1 is a Wnt pathway inhibitor with IC50 of 180 nM in L-cells expressing Wnt3A, and induces Axin2 protein levels and promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes.

    IC50 & Target

    IC50: 180 nM (Wnt)

    In Vitro

    Both IWR-1 and XAV939 act as reversible Wnt pathway inhibitors and exhibit similar pharmacological effects in vitro. IWR-1 exerts its effect via interaction with Axin, while XAV939 binds TNKS directly[1]. IWR-1 (10 μM) induces stabilization of β-catenin disruption complex. IWR-1 (10 μM) is added to the medium together with MIF, the size of cell colonies is extremely decreased, and that indicates the promoting effect of MIF on NSPC proliferation is inhibited by IWR-1 in any MIF concentration group. 2, 5 and 10 μM of IWR-1 significantly inhibits the proliferation of NSPC dose-dependently. IWR-1 inhibites the promoting effect of MIF on NSPC differentiation to neuron lineage[2]. IWR-1 treatment in the presence of maximal stimulatory dose of FSH (0.5 ng/mL) results in a dose dependent inhibition of the stimulatory effect of FSH with > 75% inhibition observed at the maximal inhibitory dose of IWR-1 (1 µM). IWR-1 treatment partially reverses the FSH-induced inhibition of granulosa cell CARTPT mRNA expression[3].

    References
    Molecular Weight

    409.44

    Formula

    C₂₅H₁₉N₃O₃

    CAS No.

    1127442-82-3

    SMILES

    O=C(NC1=C2N=CC=CC2=CC=C1)C3=CC=C(N(C([[email protected]]4([H])[[email protected]](C5)([H])C=C[[email protected]]5([H])[[email protected]]64[H])=O)C6=O)C=C3

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: ≥ 46 mg/mL

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    Purity: 99.83%

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product name

     

    Salutation

    Applicant name *

     

    Email address *

    Phone number *

     

    Organization name *

    Country or Region *

     

    Requested quantity *

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    IWR-1
    Cat. No.:
    HY-12238
    Quantity: