1. Stem Cell/Wnt
  2. Organoid Wnt
  3. IWR-1

IWR-1  (Synonyms: endo-IWR 1; IWR-1-endo)

Cat. No.: HY-12238 Purity: 99.49%
COA Handling Instructions

IWR-1 is a tankyrase inhibitor which inhibits Wnt/β-catenin signaling pathway.

For research use only. We do not sell to patients.

IWR-1 Chemical Structure

IWR-1 Chemical Structure

CAS No. : 1127442-82-3

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 73 In-stock
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10 mM * 1 mL in DMSO USD 73 In-stock
Solid
5 mg USD 66 In-stock
10 mg USD 101 In-stock
50 mg USD 455 In-stock
100 mg USD 690 In-stock
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Customer Review

Based on 32 publication(s) in Google Scholar

Top Publications Citing Use of Products

    IWR-1 purchased from MedChemExpress. Usage Cited in: Oncol Rep. 2018 Aug;40(2):877-886.  [Abstract]

    IWR-1 induces cell differentiation. (A) NB4 and (B) HL-60 cells are treated with 0 (DMSO), 5 and 10 μM IWR-1 for three days, and the expression of β-catenin and differentiation marker CD11b is assessed by western blotting.

    IWR-1 purchased from MedChemExpress. Usage Cited in: Cell Biol Int. 2017 May;41(5):534-543.  [Abstract]

    SCAPs are cultured with modified neural differentiation medium for the indicated time periods. The neuron-like cells in control group, SFRP2 over-expressed group and 10 uM IWR1-endo treated group are positive for βIII-tubulin (red) expression by using immunofluorescence staining.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    IWR-1 is a tankyrase inhibitor which inhibits Wnt/β-catenin signaling pathway.

    IC50 & Target

    IC50: 180 nM (Wnt)

    In Vitro

    Both IWR-1 and XAV939 act as reversible Wnt pathway inhibitors and exhibit similar pharmacological effects in vitro. IWR-1 exerts its effect via interaction with Axin, while XAV939 binds TNKS directly[1]. IWR-1 (10 μM) induces stabilization of β-catenin disruption complex. IWR-1 (10 μM) is added to the medium together with MIF, the size of cell colonies is extremely decreased, and that indicates the promoting effect of MIF on NSPC proliferation is inhibited by IWR-1 in any MIF concentration group. 2, 5 and 10 μM of IWR-1 significantly inhibits the proliferation of NSPC dose-dependently. IWR-1 inhibites the promoting effect of MIF on NSPC differentiation to neuron lineage[2]. IWR-1 treatment in the presence of maximal stimulatory dose of FSH (0.5 ng/mL) results in a dose dependent inhibition of the stimulatory effect of FSH with > 75% inhibition observed at the maximal inhibitory dose of IWR-1 (1 µM). IWR-1 treatment partially reverses the FSH-induced inhibition of granulosa cell CARTPT mRNA expression[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    409.44

    Formula

    C25H19N3O3

    CAS No.
    Appearance

    Solid

    Color

    Light yellow to brown

    SMILES

    O=C(NC1=C2N=CC=CC2=CC=C1)C3=CC=C(N(C([C@]4([H])[C@](C5)([H])C=C[C@]5([H])[C@]64[H])=O)C6=O)C=C3

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (122.12 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4424 mL 12.2118 mL 24.4236 mL
    5 mM 0.4885 mL 2.4424 mL 4.8847 mL
    10 mM 0.2442 mL 1.2212 mL 2.4424 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

    *All of the co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.49%

    References
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    IWR-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
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    Cat. No.:
    HY-12238
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