SFRP2 enhanced the adipogenic and neuronal differentiation potentials of stem cells from apical papilla

  • Cell Biol Int. 2017 May;41(5):534-543. doi: 10.1002/cbin.10757.
Xiao Lin  1  2 ,  Rui Dong  1 ,  Shu Diao  1  3 ,  Guoxia Yu  1  4  5 ,  Liping Wang  1 ,  Jun Li  2 ,  Zhipeng Fan  1
Affiliations
  • 1. Laboratory of Molecular Signaling and Stem Cells Therapy, Capital Medical University School of Stomatology, Beijing, China.
  • 2. Department of Implant Dentistry, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.
  • 3. Department of Pediatric Dentistry, Capital Medical University School of Stomatology, Beijing, China.
  • 4. Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.
  • 5. Department of Stomatology, Beijing Children's Hospital, Capital Medical University, Beijing, China.
PMID: 28244619 DOI: 10.1002/cbin.10757
Abstract

Dental tissue-derived mesenchymal stem cells (MSCs) are easily obtained and considered as a favorable cell source for tissue engineering, but the regulation of direct differentiation is unknown, which restricts their application. The present study investigated the effect of SFRP2, a Wnt signaling modulator, on MSC differentiation using stem cells from apical papilla (SCAPs). The cells were cultured in specific inducing medium for adipogenic, neurogenic, or chondrogenic differentiation. Over-expression of SFRP2 via retroviral Infection enhanced the adipogenic and neurogenic differentiation of SCAPs. While inhibit of Wnt pathway by IWR1-endo could enhance the neurogenic differentiation potentials of SCAPs, similar with the function of SFRP2. In addition, over-expression of SFRP2 up-regulated the expression of stemness-related genes SOX2 and OCT4. Furthermore, SOX2 and OCT4 expression was significantly inhibited after lentiviral silencing of SFRP2 in SCAPs. Therefore, our results suggest that SFRP2 enhances the adipogenic and neurogenic differentiation potentials of SCAPs by up-regulating SOX2 and OCT4. Moreover, the effect of SFRP2 in neurogenic differentiation of SCAPs maybe also associated with Wnt inhibition. Our results provided useful information about the molecular mechanism underlying directed differentiation in dental tissue-derived MSCs.

Keywords
SFRP2; adipogenic differentiation; neurogenic differentiation; stem cells from apical papilla (SCAPs); stemness.
Products