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  4. Imiquimod

Imiquimod (R 837), an immune response modifier, is a selective toll like receptor 7 (TLR7) agonist. Imiquimod exhibits antiviral and antitumor effects in vivo. Imiquimod can be used for the research of external genital, perianal warts, cancer and COVID-19.

For research use only. We do not sell to patients.

Imiquimod Chemical Structure

Imiquimod Chemical Structure

CAS No. : 99011-02-6

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500 mg USD 151 In-stock
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Customer Review

Based on 31 publication(s) in Google Scholar

Other Forms of Imiquimod:

Top Publications Citing Use of Products

30 Publications Citing Use of MCE Imiquimod

WB

    Imiquimod purchased from MedChemExpress. Usage Cited in: Nat Commun. 2016 May 25;7:11724.  [Abstract]

    Freshly prepared splenic B cells are left unstimulated (lanes 1 and 4), or stimulated by 10 μg/mL αIgM for 6 or 24 h (lanes 2 and 3), or by 1 μg/mL IMQ for 6, 24, 48 and 72 h (lanes 5-8), or by αIgM and IMQ for 24 or 48 h (lanes 9+10), respectively.

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    Description

    Imiquimod (R 837), an immune response modifier, is a selective toll like receptor 7 (TLR7) agonist. Imiquimod exhibits antiviral and antitumor effects in vivo. Imiquimod can be used for the research of external genital, perianal warts, cancer and COVID-19[1][2].

    IC50 & Target[1][3]

    TLR7

     

    HSV-1

     

    In Vivo

    Imiquimod can be used in animal modeling to construct psoriasis models.

    In animal models, Imiquimod stimulates the innate immune response by increasing NK cell activity, activating macrophages to secrete cytokines and nitric oxide, and inducing the proliferation and differentiation of B lymphocytes. Imiquimod stimulates the innate immune response through induction, synthesis, and release of cytokines, including interferon-a (IFN-α), interleukin (IL)-6, and tumor necrosis factor (TNF)-α[1].

    Lmiquimod (IMQ) is a classic psoriasis modeling agent that induces inflamed scaly skin lesions resembling plaque-type psoriasis. IMQ induces epidermal expression of IL-23, IL-17A, and IL-17F, as well as an increase in splenic Th17 cells. Rats and mice are generally used as animal models[5][6].

    Dose reference for IMQ induction[5][6]:
    (1) Model animal: BALB/c mice (8-11 week)
    Psoriasis Model: 3.125 mg/day, skin, 6 day
    (2) Model animals: Male Wistar rats (9–12 weeks old)
    Psoriasis Model: 125 mg/day, skin, 5 days

    IMQ cream preparation [7]:
    (1) Prepare BM of lipids
    STA and OA were melted at 75 °C followed by the addition of IMQ (3.5% of the BM, w/w) under continuous stirring.
    Polysorbate 80 and stearoyl polyoxyl-32 glycerides at a 50:50 ratio were dispersed in water heated separately to 75 °C and added to the BM phase under continuous stirring.
    (2) Prepare nanostructured lipid carriers (NLCs)
    The mixture was then subjected to high-shear homogenization followed by ultrasonication. The mixture was then rapidly cooled in an ice bath to allow the formation of NLCs.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: BALB/c mice (8-11 week) [5]
    Dosage: 3.125 mg/day, 6 day
    Administration: skin
    Result: Induced the back skin of the mice started to display signs of erythema, scaling, and thickening.
    Resulted in hyperproliferative keratinocytes and a disturbed epidermal differentiation (parakeratosis).
    Clinical Trial
    Molecular Weight

    240.30

    Formula

    C14H16N4

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC1=NC2=CC=CC=C2C3=C1N=CN3CC(C)C

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    H2O : 2.64 mg/mL (10.99 mM; ultrasonic and adjust pH to 2 with HCl)

    DMSO : 2 mg/mL (8.32 mM; ultrasonic and warming and heat to 70°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Methanol : < 1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.1615 mL 20.8073 mL 41.6146 mL
    5 mM 0.8323 mL 4.1615 mL 8.3229 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.96%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 4.1615 mL 20.8073 mL 41.6146 mL 104.0366 mL
    5 mM 0.8323 mL 4.1615 mL 8.3229 mL 20.8073 mL
    H2O 10 mM 0.4161 mL 2.0807 mL 4.1615 mL 10.4037 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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