Imiquimod dihydrochloride
Based on 50 publication(s) in Google Scholar
Imiquimod (R 837) dihydrochloride, an immune response modifier, is a selective toll like receptor 7 (TLR7) agonist. Imiquimod exhibits antiviral and antitumor effects in vivo. Imiquimod can be used for the research of external genital, perianal warts, cancer and COVID-19.
For research use only. We do not sell to patients.
- CAS No.: 2995277-02-4
- Formula: C14H18Cl2N4
- Molecular Weight:313.23
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Imiquimod dihydrochloride
More- Nature. 2025 May;641(8061):202-210. [Abstract]
- Nat Commun. 2016 May 25:7:11724. [Abstract]
- J Nanobiotechnology. 2022 May 14;20(1):228. [Abstract]
- Nucleic Acids Res. 2021 Jan 8;49(D1):D1113-D1121. [Abstract]
- Theranostics. 2023 Apr 9;13(7):2226-2240. [Abstract]
- Acta Pharm Sin B. 2024 Oct;14(10):4360-4377. [Abstract]
- Adv Sci (Weinh). 2024 Sep;11(35):e2405158. [Abstract]
- MedComm (2020). 2022 Mar 4;3(1):e120. [Abstract]
- Biomaterials. 2022 Mar:282:121411. [Abstract]
- Biomaterials. 2021 Apr:271:120724. [Abstract]
- Mol Biomed. 2025 Nov 13;6(1):108. [Abstract]
- J Control Release. 2023 Sep:361:443-454. [Abstract]
- Cell Death Dis. 2025 Jan 20;16(1):31. [Abstract]
- Cell Commun Signal. 2024 Nov 25;22(1):565. [Abstract]
- Cell Commun Signal. 2023 Aug 18;21(1):215. [Abstract]
- Drug Deliv. 2023 Dec;30(1):2183814. [Abstract]
- Biodes Manuf. 2025 May 22.
- Cell Rep. 2021 Feb 2;34(5):108724. [Abstract]
- J Invest Dermatol. 2022 May;142(5):1349-1359.e8. [Abstract]
- Pharmaceutics. 2025 Nov 20;17(11):1503. [Abstract]
- J Ethnopharmacol. 2026 Oct 28:369:121903. [Abstract]
- J Ethnopharmacol. 2024 Apr 6:323:117711. [Abstract]
- J Agric Food Chem. 2024 Jan 17;72(2):1055-1066. [Abstract]
- Inflamm Res. 2026 Apr 17;75(1):93. [Abstract]
- EMBO Rep. 2022 Feb 3;23(2):e53499. [Abstract]
- Int Immunopharmacol. 2024 Sep 21;142(Pt B):113190. [Abstract]
- ACS Appl Bio Mater. 2019 Feb 18;2(2):874-883. [Abstract]
- J Periodontal Res. 2025 Feb 25. [Abstract]
- Am J Respir Cell Mol Biol. 2023 Mar;68(3):256-266. [Abstract]
- Diam Relat Mater. 2023 May 26, 110058.
- Eur J Pharm Sci. 2026 Mar 19:107510. [Abstract]
- Sci Rep. 2024 Mar 4;14(1):5237. [Abstract]
- J Cell Mol Med. 2024 Apr;28(8):e18356. [Abstract]
- J Inflamm Res. 2025 Mar 20:18:4213-4231. [Abstract]
- FASEB J. 2025 Dec 15;39(23):e71278. [Abstract]
- Arch Microbiol. 2025 Oct 6;207(11):290. [Abstract]
- FEBS Lett. 2021 Oct;595(19):2447-2462. [Abstract]
- Tuberculosis. 2025 Oct 14:155:102698. [Abstract]
- Biochem Biophys Res Commun. 2024 Apr 16:704:149661. [Abstract]
- Ir J Med Sci. 2025 Dec 13. [Abstract]
- SSRN. 2025 Dec 2.
- SSRN. 2025 Jul 30.
- Patent. US20250235403A1.
- SSRN. 2025 Jun 24.
- Patent. US20240417738A1.
- Preprints. 2024 Aug 27.
- Research Square Preprint. 2023 Nov 17.
- Research Square Print. 2023 Feb 1.
- Oxid Med Cell Longev. 2022 Oct 12:2022:6264474. [Abstract]
- Research Square Preprint. 2021 Oct.
Customer Validation & Images
-
Histological Imaging/Staining
-
Bio/Physico-chemical Assay
-
Bio/Physico-chemical Assay
-
Bio/Physico-chemical Assay
-
Histological Imaging/Staining
Biological Activity
Administration: 3.125 mg/day • skin • 6 days
(1) Prepare BM of lipids
STA and OA were melted at 75 °C followed by the addition of IMQ (3.5% of the BM, w/w) under continuous stirring.
Polysorbate 80 and stearoyl polyoxyl-32 glycerides at a 50:50 ratio were dispersed in water heated separately to 75 °C and added to the BM phase under continuous stirring.
(2) Prepare nanostructured lipid carriers (NLCs)
The mixture was then subjected to high-shear homogenization followed by ultrasonication.
The mixture was then rapidly cooled in an ice bath to allow the formation of NLCs.
Resulted in hyperproliferative keratinocytes and a disturbed epidermal differentiation (parakeratosis).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 2995277-02-4
-
Molecular Weight 313.23
-
Formula C14H18Cl2N4
-
SMILES
NC1=NC2=C(C3=C1N=CN3CC(C)C)C=CC=C2.Cl.Cl
-
Synonyms
R 837 dihydrochloride
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (50)
-
Journal Impact Factor
-
Most Recent
-
Nature
2025 May;641(8061):202-210. PMID: 40140567 -
Nat Commun
NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells. [Abstract]2016 May 25:7:11724. PMID: 27222343
Imiquimod dihydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2016 May 25:7:11724. [Abstract]
Freshly prepared splenic B cells are left unstimulated (lanes 1 and 4), or stimulated by 10 μg/mL αIgM for 6 or 24 h (lanes 2 and 3), or by 1 μg/mL IMQ for 6, 24, 48 and 72 h (lanes 5-8), or by αIgM and IMQ for 24 or 48 h (lanes 9+10), respectively.
-
J Nanobiotechnology
Enhancement of antitumor immunotherapy using mitochondria-targeted cancer cell membrane-biomimetic MOF-mediated sonodynamic therapy and checkpoint blockade immunotherapy. [Abstract]2022 May 14;20(1):228. PMID: 35568916
Imiquimod dihydrochloride purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2022 May 14;20(1):228. [Abstract]
TEM image and magnified TEM Image of Zr-TCPP(TPP)/R837@M nanoparticles, scale bar: 200 nm.
Imiquimod dihydrochloride purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2022 May 14;20(1):228. [Abstract]
XPS spectra of Zr-TCPP(TPP)/R837@M.
-
Nucleic Acids Res
COVID19 Drug Repository: text-mining the literature in search of putative COVID19 therapeutics. [Abstract]2021 Jan 8;49(D1):D1113-D1121. PMID: 33166390 -
Theranostics
2023 Apr 9;13(7):2226-2240. PMID: 37153743
Imiquimod dihydrochloride purchased from MedChemExpress. Usage Cited in: Theranostics. 2023 Apr 9;13(7):2226-2240. [Abstract]
Representative images of lung of mice after indicated treatment. PFI: ICG-loaded PF127 hydrogel, PFIR: ropivacaine-loaded PFI hydrogel, PFIM: imiquimod-loaded PFI hydrogel, PFIRM: imiquimod (3 mg) and ropivacaine co-loaded PFI hydrogel.
Imiquimod dihydrochloride purchased from MedChemExpress. Usage Cited in: Theranostics. 2023 Apr 9;13(7):2226-2240. [Abstract]
1. Cumulative release profile of ICG and Imiquimod (3 mg) from PFIRM with or without laser irradiation at 1.2 W/cm2 for 5 min. The absorbance of IMQ and ICG were measured at 320 and 800 nm, respectively, using a Micro UV-Vis Spectrophotometer (LIFEREAL, FC-1100). The assay was repeated three times.
-
Acta Pharm Sin B
Macrophage P2Y6R activation aggravates psoriatic inflammation through IL-27-mediated Th1 responses. [Abstract]2024 Oct;14(10):4360-4377. PMID: 39525587 -
Adv Sci (Weinh)
Boron Neutron Capture Therapy-Derived Extracellular Vesicles via DNA Accumulation Boost Antitumor Dendritic Cell Vaccine Efficacy. [Abstract]2024 Sep;11(35):e2405158. PMID: 39021327 -
MedComm (2020)
Tyrosine phosphatase SHP2 exacerbates psoriasis-like skin inflammation in mice via ERK5-dependent NETosis. [Abstract]2022 Mar 4;3(1):e120. PMID: 35281792
Imiquimod dihydrochloride purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2022 Mar 4;3(1):e120. [Abstract]
H&E staining of back skin sections of sham‐ or IMQ‐treated SHP2N KO and WT mice, scale bar = 200 μm.
Imiquimod dihydrochloride purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2022 Mar 4;3(1):e120. [Abstract]
IMQ (10 μM, 2 h) treatment significantly increased the proportion of neutrophils in immune cells, while SHP099 treatment significantly decreased it.
Imiquimod dihydrochloride purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2022 Mar 4;3(1):e120. [Abstract]
Results showed that neutrophil infiltration occurred in the skin tissues after IMQ (10 μM, 2 h) treatment, and SHP099 significantly reversed this phenomenon.
-
Biomaterials
2022 Mar:282:121411. PMID: 35189461 -
Biomaterials
Physiologically triggered injectable red blood cell-based gel for tumor photoablation and enhanced cancer immunotherapy. [Abstract]2021 Apr:271:120724. PMID: 33636549 -
Mol Biomed
ML345 is a potent and selective NLRP3 inflammasome inhibitor with anti-inflammatory activity. [Abstract]2025 Nov 13;6(1):108. PMID: 41231359 -
J Control Release
Liposomal co-delivery of toll-like receptors 3 and 7 agonists induce a hot triple-negative breast cancer immune environment. [Abstract]2023 Sep:361:443-454. PMID: 37558053 -
Cell Death Dis
S1PR3-driven positive feedback loop sustains STAT3 activation and keratinocyte hyperproliferation in psoriasis. [Abstract]2025 Jan 20;16(1):31. PMID: 39833165 -
Cell Commun Signal
Identification of a covalent NEK7 inhibitor to alleviate NLRP3 inflammasome-driven metainflammation. [Abstract]2024 Nov 25;22(1):565. PMID: 39587676 -
Cell Commun Signal
TLR7 activation by miR-21 promotes renal fibrosis by activating the pro-inflammatory signaling pathway in tubule epithelial cells. [Abstract]2023 Aug 18;21(1):215. PMID: 37596656 -
Drug Deliv
Biomimetic virus-like mesoporous silica nanoparticles improved cellular internalization for co-delivery of antigen and agonist to enhance Tumor immunotherapy. [Abstract]2023 Dec;30(1):2183814. PMID: 36843529 -
-
Cell Rep
MyD88 in myofibroblasts enhances colitis-associated tumorigenesis via promoting macrophage M2 polarization. [Abstract]2021 Feb 2;34(5):108724. PMID: 33535045
Imiquimod dihydrochloride purchased from MedChemExpress. Usage Cited in: Cell Rep. 2021 Feb 2;34(5):108724. [Abstract]
Primary intestinal NFs were exposed to TLR agonists and IL-1 for 12 h, and OPN mRNA was measured using qPCR analysis. The results were normalized to β-actin. ∗p < 0.05. TLR agonists were used at the following concentrations: TLR1/2 agonist (Pam3CSK4), 100 ng/mL; TLR4 agonist (LPS), 1 μg/mL; TLR7 agonist (Imiquimod; HY-B0180), 100 ng/mL; TLR8 agonist (Motolimod; HY-13773), 250 ng/mL; and IL-1, 5 ng/ml.
-
J Invest Dermatol
Adipocyte-Derived CTRP3 Exhibits Anti-Inflammatory Effects via LAMP1-STAT3 Axis in Psoriasis. [Abstract]2022 May;142(5):1349-1359.e8. PMID: 34687744 -
Pharmaceutics
Functional pH-Responsive Nanoparticles for Immune Reprogramming in MSS Colorectal Cancer via ER Stress-Induced Proteostasis Disruption, PD-L1-Targeting miRNA, and TLR7 Activation. [Abstract]2025 Nov 20;17(11):1503. PMID: 41304839 -
J Ethnopharmacol
Melampodium divaricatum (Rich.) DC. extracts exert anti-inflammatory effects via inhibition of the NLRP3 inflammasome. [Abstract]2026 Oct 28:369:121903. PMID: 42202918 -
J Ethnopharmacol
Guarea microcarpa C. DC. extract inhibits NLRP3 inflammasome by suppressing its ATPase activity. [Abstract]2024 Apr 6:323:117711. PMID: 38176663 -
J Agric Food Chem
Yogurt Alleviates Imiquimod-Induced Psoriasis by Activating the Lactate/GPR81 Signaling Axis in Mice. [Abstract]2024 Jan 17;72(2):1055-1066. PMID: 38170675 -
Inflamm Res
2026 Apr 17;75(1):93. PMID: 41998354 -
EMBO Rep
Licochalcone B specifically inhibits the NLRP3 inflammasome by disrupting NEK7-NLRP3 interaction. [Abstract]2022 Feb 3;23(2):e53499. PMID: 34882936 -
Int Immunopharmacol
Casting NETs on Psoriasis: The modulation of inflammatory feedback targeting IL-36/IL-36R axis. [Abstract]2024 Sep 21;142(Pt B):113190. PMID: 39306890 -
ACS Appl Bio Mater
Polydopamine-Based Multifunctional Platform for Combined Photothermal Therapy, Chemotherapy, and Immunotherapy in Malignant Tumor Treatment. [Abstract]2019 Feb 18;2(2):874-883. PMID: 35016291 -
J Periodontal Res
Toll-Like Receptor 7 Promotes Periodontal Inflammation and Alveolar Bone Resorption Through the NF-κB Signaling Pathway. [Abstract]2025 Feb 25. PMID: 40007244 -
Am J Respir Cell Mol Biol
Neuromedin U Induces Pulmonary ILC2 Activation via the NMUR1 Pathway During Acute RSV Infection. [Abstract]2023 Mar;68(3):256-266. PMID: 36227802 -
-
Eur J Pharm Sci
In Vitro Investigation of Tapinarof-Loaded Nanogels in an Imiquimod-induced HaCaT-THP-1 Co-Culture Model. [Abstract]2026 Mar 19:107510. PMID: 41864521 -
Sci Rep
Aiouea padiformis extract exhibits anti-inflammatory effects by inhibiting the ATPase activity of NLRP3. [Abstract]2024 Mar 4;14(1):5237. PMID: 38433281 -
J Cell Mol Med
Anti-inflammatory effect of Trichospira verticillata via suppression of the NLRP3 inflammasome in neutrophilic asthma. [Abstract]2024 Apr;28(8):e18356. PMID: 38668995 -
J Inflamm Res
2025 Mar 20:18:4213-4231. PMID: 40129872 -
FASEB J
Biphasic Regulation of TLR7/MyD88/IRF7-IFN-β Axis by Toxoplasma gondii Facilitates Immune Evasion. [Abstract]2025 Dec 15;39(23):e71278. PMID: 41307494 -
Arch Microbiol
A BCG-boosting subunit vaccine RS2/DMI induces Th1-polarized immunity by targeting RD6 and secretory antigens of Mycobacterium tuberculosis. [Abstract]2025 Oct 6;207(11):290. PMID: 41051521 -
FEBS Lett
2021 Oct;595(19):2447-2462. PMID: 34387860 -
Tuberculosis
Diagnostic value of five Mycobacterium tuberculosis dormant highly expressed antigens in latent infections and immunogenicity assessment of a novel subunit vaccine PB2-DIMQ. [Abstract]2025 Oct 14:155:102698. PMID: 41108940 -
Biochem Biophys Res Commun
Sequential administration of delta-tocotrienol ameliorates radiation-induced myelosuppression in mice and non-human primates through inducing G-CSF production. [Abstract]2024 Apr 16:704:149661. PMID: 38417343 -
Ir J Med Sci
TLR7 modulates glioblastoma progression through PI3K/AKT/mTOR pathway and immune microenvironment remodeling. [Abstract]2025 Dec 13. PMID: 41389249 -
-
-
-
-
-
-
-
-
Oxid Med Cell Longev
miR-124-3p Delivered Using Exosomes Attenuates the Keratinocyte Response to IL-17A Stimulation in Psoriasis. [Abstract]2022 Oct 12:2022:6264474. PMID: 36275890 -
Purity & Documentation
References
[1]. Athina Angelopoulou, et al. Imiquimod - A toll like receptor 7 agonist - Is an ideal option for management of COVID 19. Environ Res. 2020 Sep; 188: 109858. [Content Brief]
[2]. Aditya K Gupta, et al. Imiquimod: a review. J Cutan Med Surg. Nov-Dec 2002;6(6):554-60. [Content Brief]
[3]. Yuji Kan, et al. Imiquimod suppresses propagation of herpes simplex virus 1 by upregulation of cystatin A via the adenosine receptor A1 pathway. J Virol. 2012 Oct;86(19):10338-46. [Content Brief]
[4]. Michael P Schön, et al. The small antitumoral immune response modifier imiquimod interacts with adenosine receptor signaling in a TLR7- and TLR8-independent fashion. J Invest Dermatol. 2006 Jun;126(6):1338-47. [Content Brief]
[5]. Leslie van der Fits, et al. Imiquimod-induced psoriasis-like skin inflammation in mice is mediated via the IL-23/IL-17 axis. J Immunol. 2009, 182, 9. [Content Brief]
[6]. Ajla Smajlović, et al. Molecular and histopathological profiling of imiquimod induced dermatosis in Swiss Wistar rats: contribution to the rat model for novel anti-psoriasis treatments. Mol Biol Rep. 2021, 48, 5. [Content Brief]
[7]. Sangseo Kim, et al. Development and Optimization of Imiquimod-Loaded Nanostructured Lipid Carriers Using a Hybrid Design of Experiments Approach. Int J Nanomedicine. 2023. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Imiquimod dihydrochloride Related Classifications
HY-B0180C Related Classifications
Powered by Bioz