2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Menaquinone-4

Menaquinone-4  (Synonyms: Vitamin K2(MK-4); Menaquinone K4)

Cat. No.: HY-B2156 Purity: 99.96%
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Menaquinone-4 is a vitamin K, used as a hemostatic agent, and also a adjunctive therapy for the pain of osteoporosis.

For research use only. We do not sell to patients.

CAS No. : 863-61-6

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10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Menaquinone-4:

Menaquinone-4 Related Antibodies

Top Publications Citing Use of Products

    Menaquinone-4 purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2025 Jun 23.  [Abstract]

    Cell viability analysis of SK-HEP-1 cells after treatment with 10 μM (20)S-APPT alone or simultaneously with various VKs (VK1 at 10 μM, Menaquinone-4 (VK2) at 10 μM, VK3 at 5 μM, and MK4 at 5 μM). The results demonstrated that VK2 could also serve as a substrate for FSP1, significantly attenuating cell death induced by (20)S-APPT.

    Menaquinone-4 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 May;15(5):101092.

    Menaquinone-4 (VK2, 0.5-40 μM; 24 h). Evaluation of cellular toxicity of vitamin K. Chondrocytes were treated with indicated vitamin K concentrations for 24 h.

    Menaquinone-4 purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2024 Oct 24:17:7675-7685.  [Abstract]

    Immunofluorescence staining and quantitative detection of ROS levels in lung tissue. Magnification: ×200 (n=3). The results showed that the ROS level was significantly increased in the sepsis group (CLP group) compared with the control group or the sham-operated group. However, the ROS level in the Menaquinone-4 (MK-4, 200 mg/kg; i.p.; once daily for 3 days) + CLP group was markedly lower than that in the CLP group, suggesting that MK-4 ameliorated oxidative stress in SI-ALI in C57BL/6N mice.CLP: Cecal ligation and puncture.

    Menaquinone-4 purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2024 Oct 24:17:7675-7685.  [Abstract]

    ELISA was used to measure the levels of IL-6 and TNF-α in cardiac blood (n=3). The results indicated that Menaquinone-4 (MK‑4, 200 mg/kg; i.p.; once daily for 3 days) reduced the levels of inflammatory cytokines in SI‑ALI in C57BL/6N mice. CLP: Cecal ligation and puncture.

    Menaquinone-4 purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2024 Oct 24:17:7675-7685.  [Abstract]

    Menaquinone-4 (MK-4, 200 mg/kg; i.p.; once daily for 3 days) attenuated cecal ligation and puncture (CLP)-induced p53 acetylation in C57BL/6N mice.

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    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Menaquinone-4 is a vitamin K, used as a hemostatic agent, and also a adjunctive therapy for the pain of osteoporosis.

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Menaquinone-4 (MK-4, 0, 1, 5, 10 μM) increases the ALP activity in Caco-2 cells. Menaquinone-4 (1 μM) significantly increases intensities of hSI expression[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Menaquinone-4 Related Antibodies
    In Vivo

    Menaquinone-4 (K2, 0.2 g/kg diet) in HF-K2 group produces epididymal fat in C57BL/6J mice, and also increases the bone density of mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    444.65

    Formula

    C31H40O2
    CAS No.
    Appearance

    <35°C Solid,>35°C Liquid

    Color

    Light yellow to yellow

    SMILES

    O=C1C(C)=C(C/C=C(C)/CC/C=C(C)/CC/C=C(C)/CC/C=C(C)\C)C(C2=C1C=CC=C2)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 10 mg/mL (22.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2490 mL 11.2448 mL 22.4896 mL
    5 mM 0.4498 mL 2.2490 mL 4.4979 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    C2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (4.68 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2 mg/mL (4.50 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.96%

    SDS (394 KB)

    COA (241 KB) HNMR (195 KB) RP-HPLC (247 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    Caco-2 cells are plated at a density of 2-5 × 104 cells/cm2 onto a 35-mm dish. Cells are incubated for 2 to 3 days until 60%-70% confluency, and desired concentrations of Menaquinone-4 (0, 1.0, 5.0, and 10.0 μM) are added. The final concentration of the vehicle is 0.1% of the culture medium, and the culture medium is changed twice a week. Cells are assayed on days 0, 3, 7, and 11 after the addition of Menaquinone-4[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Forty-two male, 4-week-old C57BL/6J mice provided with feed and drink ad libitum. For environmental adaptation, the animals had 1 week of circulation, and then are provided with the experimental diet after being randomly divided into 6 groups (7 animals in each group; randomized block design). The AIN-93G diets consist of a normal diet (N), normal diet + vitamin K1 (N-K1), normal diet + vitamin Menaquinone-4 (N-K2), 45% high-fat diet (HF), 45% high-fat diet + vitamin K1 (HF-K1), and a 45% high-fat diet + vitamin Menaquinone-4 (HF-K2). The vitamin K1 and vitamin Menaquinone-4 contents are 200 mg/1,000 g, and the diet is provided in pellet form. Body weight is measured once a week, and the food efficiency ratio (FER) is calculated by dividing the increased body weight from day 1 to the final day by the food intake amount during the experiment period. For fat amount measurement, the epididymal fat, perirenal fat, and retroperitoneal fat are extracted from dead animal subjects, are washed with 0.9% NaCl, dried by filter paper, and then are weighed[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2490 mL 11.2448 mL 22.4896 mL 56.2240 mL
    5 mM 0.4498 mL 2.2490 mL 4.4979 mL 11.2448 mL
    10 mM 0.2249 mL 1.1245 mL 2.2490 mL 5.6224 mL
    15 mM 0.1499 mL 0.7497 mL 1.4993 mL 3.7483 mL
    20 mM 0.1124 mL 0.5622 mL 1.1245 mL 2.8112 mL
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    Menaquinone-4 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Menaquinone-4863-61-6Vitamin K2(MK-4) Menaquinone K4Menaquinone4Menaquinone 4Menaquinone K4Menaquinone K 4Menaquinone K-4Endogenous MetaboliteInhibitorinhibitorinhibit

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