NS309

Name: NS309
Brand: MedChemExpress
SKU: HY-15416
Rating: 4.5 (0 reviews)

Cat. No.: HY-15416 Purity: 99.09%: Data Sheet
COA

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Handling Instructions
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NS309 is a potent and selective activator of the Ca²⁺-activated SK/IK potassium channels, but displays no activity at BK channels.

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NS309 화학구조

CAS No. : 18711-16-5

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

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This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 Customer Validation

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

NS309 is a potent and selective activator of the Ca²⁺-activated SK/IK potassium channels, but displays no activity at BK channels^[1]^[2]^[3]^[4].

IC₅₀ & Target

EC50: 0.62 μM (SK2)^[1], 0.3 μM (SK3)^[2], ～20 nM (K_Ca3.1/SK4)^[3], ～600 nM (K_Ca2/SK channels)^[3], 10 nM (hIK)^[4]

In Vitro

NS309 (40 nM) activates both hSK3 and hIK channels, with the largest effect on hIK channels (8.5-fold increase in current compares to 1.9-fold increase in current for hSK3 channels)^[2].
NS309 (10 μM) significantly increases the whole cell SK currents and hyperpolarized detrusor smooth muscle (DSM) cells resting membrane potential^[5].
NS309 inhibits the spontaneous phasic contraction amplitude, force, frequency, duration and tone of isolated DSM strips in a concentration-dependent manner^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

NS309 (2 mg/kg; i.p.) protects against SCI/R in rabbits^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Adult New Zealand white rabbits (2.5-3.0 kg)^[6]
Dosage:	2 mg/kg
Administration:	Intraperitoneal injection
Result:	Significantly improved neurological outcome of SCI/R challenged rabbits.

분자량

231.04

화학식

C₈H₄Cl₂N₂O₂

CAS No.

18711-16-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1NC2=C(C=CC(Cl)=C2Cl)/C1=N/O

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

용액&용해도

In Vitro:

DMSO : 100 mg/mL (432.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.3283 mL	21.6413 mL	43.2826 mL
5 mM	0.8657 mL	4.3283 mL	8.6565 mL
10 mM	0.4328 mL	2.1641 mL	4.3283 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

순도&문서

Purity: 99.09%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (251 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Specific enhancement of SK channel activity selectively potentiates the afterhyperpolarizing current I(AHP) and modulates the firing properties of hippocampal pyramidal neurons. [Content Brief]

[2]. C Hougaard, et al. Selective positive modulation of the SK3 and SK2 subtypes of small conductance Ca2+-activated K+ channels. Br J Pharmacol. 2007 Jul; 151(5): 655–665. [Content Brief]

[3]. Nichole Coleman, et al. New Positive Ca2+-Activated K+ Channel Gating Modulators with Selectivity for KCa3.1. Mol Pharmacol. 2014 Sep; 86(3): 342–357. [Content Brief]

[4]. Dorte Strøbaek, et al. Activation of human IK and SK Ca2+-activated K+ channels by NS309 (6,7-dichloro-1H-indole-2,3-dione 3-oxime). Biochim Biophys Acta. 2004 Oct 11;1665(1-2):1-5. [Content Brief]

[5]. Shankar P Parajuli, et al. NS309 decreases rat detrusor smooth muscle membrane potential and phasic contractions by activating SK3 channels. Br J Pharmacol. 2013 Apr; 168(7): 1611–1625. [Content Brief]

[6]. Jie Zhu, et al. Activation of SK/KCa Channel Attenuates Spinal Cord Ischemia-Reperfusion Injury via Anti-oxidative Activity and Inhibition of Mitochondrial Dysfunction in Rabbits. Front Pharmacol. 2019; 10: 325. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.3283 mL	21.6413 mL	43.2825 mL	108.2064 mL
	5 mM	0.8657 mL	4.3283 mL	8.6565 mL	21.6413 mL
	10 mM	0.4328 mL	2.1641 mL	4.3283 mL	10.8206 mL
	15 mM	0.2886 mL	1.4428 mL	2.8855 mL	7.2138 mL
	20 mM	0.2164 mL	1.0821 mL	2.1641 mL	5.4103 mL
	25 mM	0.1731 mL	0.8657 mL	1.7313 mL	4.3283 mL
	30 mM	0.1443 mL	0.7214 mL	1.4428 mL	3.6069 mL
	40 mM	0.1082 mL	0.5410 mL	1.0821 mL	2.7052 mL
	50 mM	0.0866 mL	0.4328 mL	0.8657 mL	2.1641 mL
	60 mM	0.0721 mL	0.3607 mL	0.7214 mL	1.8034 mL
	80 mM	0.0541 mL	0.2705 mL	0.5410 mL	1.3526 mL
	100 mM	0.0433 mL	0.2164 mL	0.4328 mL	1.0821 mL

NS309 Related Classifications

Neurological Disease Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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NS309

고객리뷰

NS309 Related Antibodies

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•Related Screening Libraries:

•Related Small Molecules:

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Biological Activity

순도&문서

References

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NS309 Related Antibodies

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Complete Stock Solution Preparation Table

NS309 Related Classifications

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