2. Autophagy
  3. Autophagy
  4. QX77

QX77 is a chaperone-mediated autophagy (CMA) activator and upregulates LAMP2A expression in vitro. QX77 induces Rab11 upregulation, rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells. QX77 can impede self-renewal and promote differentiation of ES cells.

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QX77

QX77 構造式

CAS 番号 : 1798331-92-6

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 66 在庫あり
Solution
10 mM * 1 mL in DMSO USD 66 在庫あり
Solid
5 mg $60 在庫あり
10 mg $95 在庫あり
25 mg $185 在庫あり
50 mg $295 在庫あり
100 mg $475 在庫あり
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カスタマーレビュー

Based on 5 publication(s) in Google Scholar

QX77 関連抗体

Top Publications Citing Use of Products

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

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製品説明

QX77 is a chaperone-mediated autophagy (CMA) activator and upregulates LAMP2A expression in vitro. QX77 induces Rab11 upregulation, rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells[1]. QX77 can impede self-renewal and promote differentiation of ES cells[2].

IC50 & Target

CMA[1]
体外実験

QX77 (48 hours) upregulates Rab11 expression levels in Ctns-/-?MEFs[1].
? In Ctns-/- MEFs, QX77 recovers the down-regulated LAMP2A expression, and the Rab11-positive carrier vesicles recover the high-motility trafficking phenotype observe in wild-type cells[1].
Treatment with CMA activator QX77 rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells. QX77 treatment also increases LAMP2A localization at the lysosomal membrane[1].
QX77 significantly increases the re-localization of LAMP2A at LAMP1-positive lysosomes in cystinotic cell, it corrects the localization of LAMP2A at the lysosomal membrane in cystinotic cells[1].
? QX77 protects cystinotic cells from the increased susceptibility to? tert-butyl-hydroperoxide-induced oxidative stress and reconstitutes the resistant levels observed in wild-type cells. The effect of QX77 on cystinotic cell survival is dependent on LAMP2A expression[1].
QX77 (10 μM;? 0, 3 or 6 days) activates CMA and increases LAMP2A expression in? D3 and E14 ES cell, it downregulates pluripotency factors and AP reactivity and partially lost the characteristic ES cell morphology[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

QX77 関連抗体

Western Blot Analysis[2]

Cell Line: ES D3 cell line; E14TG2a (E14) cell line
Concentration: 10 μM
Incubation Time: 3 or 6 days
Result: Increased LAMP2A expression and decreased SOX2 and Oct4 protein expression.
分子量

300.74

分子式

C16H13ClN2O2
CAS 番号
Appearance

Solid

Color

Light yellow to yellow

SMILES

CC(NC1=CC=C(C2=NC3=CC=C(Cl)C=C3OC2)C=C1)=O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 15 mg/mL (49.88 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3251 mL 16.6257 mL 33.2513 mL
5 mM 0.6650 mL 3.3251 mL 6.6503 mL
10 mM 0.3325 mL 1.6626 mL 3.3251 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

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体積 （開始）

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体内:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  50% PEG300    50% Saline

    Solubility: 10 mg/mL (33.25 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Working solution concentration: mg/mL
純度とドキュメンテーション

純度: 99.48%

COA (245 KB) HNMR (255 KB) LCMS (266 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3251 mL 16.6257 mL 33.2513 mL 83.1283 mL
5 mM 0.6650 mL 3.3251 mL 6.6503 mL 16.6257 mL
10 mM 0.3325 mL 1.6626 mL 3.3251 mL 8.3128 mL
15 mM 0.2217 mL 1.1084 mL 2.2168 mL 5.5419 mL
20 mM 0.1663 mL 0.8313 mL 1.6626 mL 4.1564 mL
25 mM 0.1330 mL 0.6650 mL 1.3301 mL 3.3251 mL
30 mM 0.1108 mL 0.5542 mL 1.1084 mL 2.7709 mL
40 mM 0.0831 mL 0.4156 mL 0.8313 mL 2.0782 mL
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QX77 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

QX771798331-92-6QX 77QX-77AutophagyRab11MEFsLAMP2ACMApluripotencyESInhibitorinhibitorinhibit

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製品名:
QX77
製品番号:
HY-112483
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