Sulfo-SMCC sodium

Name: Sulfo-SMCC sodium
Brand: MedChemExpress
SKU: HY-D0975
Rating: 5.0 (5 reviews)

Cat. No.: HY-D0975 Purity: 99.86%: Data Sheet
COA

SDS
Handling Instructions
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Sulfo-SMCC sodium is a commonly used hetero-bifunctional, noncleavable ADC crosslinker bearing N-hydroxysuccinimide (NHS) ester and maleimide groups to react with primary amines and sulfhydryl groups, respectively.

For research use only. We do not sell to patients.

CAS No. : 92921-24-9

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5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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500 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 5 publication(s) in Google Scholar

5 Publications Citing Use of MCE Sulfo-SMCC sodium

Gel Electrophoresis

Bio/Physico-chemical Assay

: Sulfo-SMCC sodium purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Sep 19:102365. [Abstract]; SDS-PAGE analysis of FL-Fc, FL-Fc-SMCC, and FL-Fc-DM1 under reducing and non-reducing conditions. Lanes 1 and 4: rhFL-Fc; lanes 2 and 5: FL-Fc-SMCC; lanes 3 and 6: FL-Fc-DM1.
FL-Fc (1 mg/mL) and DM1 were conjugated using the crosslinker Sulfo-SMCC. FL-Fc was mixed with Sulfo-SMCC at a 1:15 molar ratio and incubated for 0.5 h. The resulting FL-Fc-SMCC was buffer-exchanged into PE buffer (80 nM sodium phosphate, pH 6.0, 1 mM EDTA) by ultrafiltration (10 kDa cutoff). DM1 (10 mM) was then added at a 15:1 molar ratio to FL-Fc-SMCC and reacted for 1 h. Unconjugated DM1 was removed by ultrafiltration (10 kDa cutoff), and the final conjugate FL-Fc-DM1 was analyzed by SDS-PAGE.

: Sulfo-SMCC sodium purchased from MedChemExpress. Usage Cited in: BMC Med. 2022 Aug 24;20(1):257 [Abstract]; SDS-PAGE result of SCF and DM1 conjugation product. Lane 1: protein marker; lane 2: SCF and DM1 conjugation product; lane 3: rhSCF.
rhSCF was conjugated with DM1 via the Sulfo-SMCC (SMCC) chemical linker (10 mM; the molar ratio of Sulfo-SMCC to rhSCF (1 mg/mL) was 20:1; incubation for 1 h). When SCF-SMCC reacted with DM1 (10 mM), 10% DMA was added to prevent precipitation of DM1 in the aqueous system, while denaturation of rhSCF caused by DMA was minimized. The final reaction product SCF-DM1 was a mixture with a higher molecular weight than rhSCF.

: Sulfo-SMCC sodium purchased from MedChemExpress. Usage Cited in: BMC Med. 2022 Aug 24;20(1):257 [Abstract]; LC–MS result of SCF and DM1 conjugation product. + X DM1 means one molecule of SCF was conjugated with X molecule(s) of DM1, + 1 SMCC means one molecule of SCF was conjugated with 1 molecule of SMCC. LC–MS confirmed that reaction product was nonhomogeneous, with 1–5 molecule(s) of DM1 conjugated to one molecule of rhSCF.
rhSCF was conjugated with DM1 via the Sulfo-SMCC (SMCC) chemical linker (10 mM; the molar ratio of Sulfo-SMCC to rhSCF (1 mg/mL) was 20:1; incubation for 1 h). When SCF-SMCC reacted with DM1 (10 mM), 10% DMA was added to prevent precipitation of DM1 in the aqueous system, while denaturation of rhSCF caused by DMA was minimized.

: Sulfo-SMCC sodium purchased from MedChemExpress. Usage Cited in: Microb Cell Fact. 2021 Mar 10;20(1):67. [Abstract]; Reducing SDS-PAGE result of FL-DM1. Lane 1: FL-DM1; Lane 2: rhFL.
To primarily characterize the molecular weight of FL-DM1, an uncleavable Sulfo-SMCC sodium linker (5 mM, dissolved in ultrapure water) was used to generate the FL‑SMCC-DM1 drug conjugate. FL-SMCC-DM1 migrated at approximately 21 kDa, and one conjugated SMCC-DM1 molecule was approximately 960 Da. Therefore, an average of 2 SMCC‑DM1 molecules were conjugated to one rhFL molecule using this conjugation method.

: Sulfo-SMCC sodium purchased from MedChemExpress. Usage Cited in: Microb Cell Fact. 2021 Mar 10;20(1):67. [Abstract]; Reducing SDS-PAGE result of FL-DM1. Lane 1: FL-DM1; Lane 2: rhFL.
To primarily characterize the molecular weight of FL‑DM1, an uncleavable Sulfo-SMCC sodium linker (5 mM, dissolved in ultrapure water) was used to generate the FL-SMCC-DM1 drug conjugate. Based on the results of reducing SDS‑PAGE, FL had a theoretical molecular weight of 19.1 kDa (with 3 extra amino acids at its N‑terminus and 6 histidines at its C-terminus) and yielded a single band, whereas FL-SMCC-DM1 showed a smeared band, indicating a heterogeneous mixture.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Non-cleavable Linker

In Vitro

The crosslinker Sulfo-SMCC consists of a maleimide and an N-hydroxysuccinimide ester group to bind to sulfhydryl groups and primary amines, respectively. Sulfo-SMCC inhibits the end-to-end annealing of stabilized Microtubules (MTs). MTs are treated with 250 μM Sulfo-SMCC, and imaged after incubation for 0 h, 6 h, and 24 h. MTs treated with Sulfo-SMCC shows a constant mean length, independent of the incubation time^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

436.37

Formula

C₁₆H₁₇N₂NaO₉S

CAS No.

92921-24-9

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1CCC(CN2C(C=CC2=O)=O)CC1)ON3C(C(S(=O)(O[Na])=O)CC3=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (229.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 12.5 mg/mL (28.65 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2916 mL	11.4582 mL	22.9163 mL
5 mM	0.4583 mL	2.2916 mL	4.5833 mL
10 mM	0.2292 mL	1.1458 mL	2.2916 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 4.17 mg/mL (9.56 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (275 KB) SDS (394 KB)

COA (250 KB) HNMR (302 KB) RP-HPLC (240 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Prabhune M, et al. Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro. PLoS One. 2016 Aug 25;11(8):e0161623. [Content Brief]

Kinase Assay
^[1]

Labeled and unlabeled porcine tubulin powders are used. Sulfo-SMCC is used. MTs are polymerized to a final concentration of 2 mg/mL in 80 mM PIPES buffer (pH 6.8) containing 10 μM taxol, 2 mM MgCl₂, 0.5 mM EGTA, and 1 mM GTP. A mixture of rhodamine-labeled tubulin and unlabeled tubulin (1:5) is used in the time-dependent measurements. In these experiments, polymerized MTs are divided in 2 groups: control and Sulfo-SMCC (250 μM) treated. Both samples are imaged after diluting 1:5 and incubating for 0 h (within 45 minutes after polymerization), 6 h, and 24 h^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Prabhune M, et al. Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro. PLoS One. 2016 Aug 25;11(8):e0161623. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.2916 mL	11.4582 mL	22.9163 mL	57.2908 mL
	5 mM	0.4583 mL	2.2916 mL	4.5833 mL	11.4582 mL
	10 mM	0.2292 mL	1.1458 mL	2.2916 mL	5.7291 mL
	15 mM	0.1528 mL	0.7639 mL	1.5278 mL	3.8194 mL
	20 mM	0.1146 mL	0.5729 mL	1.1458 mL	2.8645 mL
	25 mM	0.0917 mL	0.4583 mL	0.9167 mL	2.2916 mL
DMSO	30 mM	0.0764 mL	0.3819 mL	0.7639 mL	1.9097 mL
	40 mM	0.0573 mL	0.2865 mL	0.5729 mL	1.4323 mL
	50 mM	0.0458 mL	0.2292 mL	0.4583 mL	1.1458 mL
	60 mM	0.0382 mL	0.1910 mL	0.3819 mL	0.9548 mL
	80 mM	0.0286 mL	0.1432 mL	0.2865 mL	0.7161 mL
	100 mM	0.0229 mL	0.1146 mL	0.2292 mL	0.5729 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.