U18666A

Name: U18666A
Brand: MedChemExpress
SKU: HY-107433
Rating: 5.0 (23 reviews)

Cat. No.: HY-107433 Purity: 99.92%: Data Sheet
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U18666A, an intra-cellular cholesterol transport inhibitor, inhibits replication of Ebola virus, dengue virus, and human hepatitis C virus.

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CAS No. : 3039-71-2

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 23 publication(s) in Google Scholar

Other Forms of U18666A:

U18666A (Standard) 견적 받기

23 Publications Citing Use of MCE U18666A

ELISA

Bio/Physico-chemical Assay

Cell Imaging/Staining

: U18666A purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jul 26;15(1):6311. [Abstract]; Immunocolocalization of RSV proteins (RSV-F, RSV-G, and RSV-N), cholesterol (filipin III), and LAMP-1 in mock-infected, RSV-infected (24 h post-infection), and U18666A (10 μM)-treated HEp-2 cells.

: U18666A purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jul 26;15(1):6311. [Abstract]; Immunocolocalization of RSV proteins (RSV-F, RSV-G, and RSV-N), cholesterol (filipin III), and LAMP-1 in mock-infected, RSV-infected (24 h post-infection), and U18666A (10 μM)-treated 16HBE cells.

: U18666A purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Jul 6;81(1):289. [Abstract]; Intracellular cholesterol contents measured in SHEDs with different treatment. Cells added U18666A (2 µg/mL), an inhibitor specific to cholesterol synthesis, were measured as positive reference.

: U18666A purchased from MedChemExpress. Usage Cited in: Emerg Microbes Infect. 2023 Dec;12(2):2261556. [Abstract]; HepG2.2.15 cells were treated with 10 μM dynasore, 2 μM U18666A, or DMSO for 48 hours. The levels of secreted HBsAg in the culture supernatant and intracellular HBsAg in the cell lysate were detected by ELISA.

: U18666A purchased from MedChemExpress. Usage Cited in: Emerg Microbes Infect. 2023 Dec;12(2):2261556. [Abstract]; HepG2.2.15 cells were treated with 10 μM dynasore, 2 μM U18666A, or DMSO for 48 hours, and HBcAg was detected by WB.

: U18666A purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2023 Jan:209:105497. [Abstract]; Pretreatment with AY9944 (15, 30 µM), U18666A (5, 10 µM), lovastatin and MβCD for 2 h can all significantly reduce the level of EV-A71 VP1 protein in HCT-8 cell.

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

U18666A, an intra-cellular cholesterol transport inhibitor, inhibits replication of Ebola virus, dengue virus, and human hepatitis C virus^[1].

IC₅₀ & Target

Cholesterol^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	> 50 3 Compound: U18666A	Cytotoxicity in human HL60 cells by MTT assay Cytotoxicity in human HL60 cells by MTT assay	[PMID: 28964935]
HL-60	IC₅₀	> 50 3 Compound: U18666A	Cytotoxicity in human HL60 cells by MTT assay Cytotoxicity in human HL60 cells by MTT assay	[PMID: 28964935]
HL-60	IC₅₀	>50 3 Compound: U18666A	Cytotoxicity in human HL60 cells by MTT assay Cytotoxicity in human HL60 cells by MTT assay	[PMID: 28964935]
Huh-7	CC₅₀	34.4 3 Compound: 67	Cytotoxicity against human HuH7 cells assessed as cell viability after 48 hrs by CellTiter Glo assay Cytotoxicity against human HuH7 cells assessed as cell viability after 48 hrs by CellTiter Glo assay	[PMID: 26771861]
Huh-7	CC₅₀	34.4 3 Compound: 67	Cytotoxicity against human HuH7 cells assessed as cell viability after 48 hrs by CellTiter Glo assay Cytotoxicity against human HuH7 cells assessed as cell viability after 48 hrs by CellTiter Glo assay	[PMID: 26771861]
Huh-7	CC₅₀	34.4 3 Compound: 67	Cytotoxicity against human HuH7 cells assessed as cell viability after 48 hrs by CellTiter Glo assay Cytotoxicity against human HuH7 cells assessed as cell viability after 48 hrs by CellTiter Glo assay	[PMID: 26771861]

In Vitro

U18666A, the antiviral effect is found to result from two events: retarded viral trafficking in the cholesterol-loaded late endosomes/lysosomes and suppresse de novo sterol biosynthesis in treated infected cells. It is also observed an additive antiviral effect of U18666A with C75, a fatty acid synthase inhibitor, suggesting dengue virus relies on both the host cholesterol and fatty acid biosynthesis for successful replication^[1]^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

424.06

화학식

C₂₅H₄₂ClNO₂

CAS No.

3039-71-2

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@]3([H])[C@](CC=C1C[C@@H](OCCN(CC)CC)CC2)([H])[C@@]4([H])[C@@](C)(C(CC4)=O)CC3.Cl

선적

Room temperature in continental US; may vary elsewhere.

보관

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

용액&용해도

In Vitro:

DMSO : 20.83 mg/mL (49.12 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 5.56 mg/mL (13.11 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3582 mL	11.7908 mL	23.5816 mL
5 mM	0.4716 mL	2.3582 mL	4.7163 mL
10 mM	0.2358 mL	1.1791 mL	2.3582 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 5 mg/mL (11.79 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.92%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (247 KB) HNMR (144 KB) RP-HPLC (243 KB) MS (153 KB)

Handling Instructions (2659 KB)

References

[1]. Poh MK, et al. U18666A, an intra-cellular cholesterol transport inhibitor, inhibits dengue virus entry and replication. Antiviral Res. 2012 Jan;93(1):191-8. [Content Brief]

[2]. Cenedella RJ, et al. Cholesterol synthesis inhibitor U18666A and the role of sterol metabolism and trafficking in numerous pathophysiological processes. Lipids. 2009 Jun;44(6):477-87. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.3582 mL	11.7908 mL	23.5816 mL	58.9539 mL
	5 mM	0.4716 mL	2.3582 mL	4.7163 mL	11.7908 mL
	10 mM	0.2358 mL	1.1791 mL	2.3582 mL	5.8954 mL
DMSO	15 mM	0.1572 mL	0.7861 mL	1.5721 mL	3.9303 mL
	20 mM	0.1179 mL	0.5895 mL	1.1791 mL	2.9477 mL
	25 mM	0.0943 mL	0.4716 mL	0.9433 mL	2.3582 mL
	30 mM	0.0786 mL	0.3930 mL	0.7861 mL	1.9651 mL
	40 mM	0.0590 mL	0.2948 mL	0.5895 mL	1.4738 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

U18666A Related Classifications

Neurological Disease Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

U18666A3039-71-2Dengue VirusFlavivirusHCVDENVHepatitis C virusInhibitorinhibitorinhibit

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U18666A

고객리뷰

Other Forms of U18666A:

U18666A Related Antibodies

23 Publications Citing Use of MCE U18666A

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

순도&문서

References

고객리뷰

U18666A Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

U18666A Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report