2. Anti-infection
  3. Dengue Virus Flavivirus HCV
  4. U18666A

U18666A, an intra-cellular cholesterol transport inhibitor, inhibits replication of Ebola virus, dengue virus, and human hepatitis C virus.

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CAS 番号 : 3039-71-2

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 88 在庫あり
Solution
10 mM * 1 mL in DMSO USD 88 在庫あり
Solid
5 mg $80 在庫あり
10 mg $130 在庫あり
25 mg $244 在庫あり
50 mg $366 在庫あり
100 mg $550 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 23 publication(s) in Google Scholar

Other Forms of U18666A:

U18666A 関連抗体

Top Publications Citing Use of Products

    U18666A purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jul 26;15(1):6311.  [Abstract]

    Immunocolocalization of RSV proteins (RSV-F, RSV-G, and RSV-N), cholesterol (filipin III), and LAMP-1 in mock-infected, RSV-infected (24 h post-infection), and U18666A (10 μM)-treated HEp-2 cells.

    U18666A purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jul 26;15(1):6311.  [Abstract]

    Immunocolocalization of RSV proteins (RSV-F, RSV-G, and RSV-N), cholesterol (filipin III), and LAMP-1 in mock-infected, RSV-infected (24 h post-infection), and U18666A (10 μM)-treated 16HBE cells.

    U18666A purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Jul 6;81(1):289.  [Abstract]

    Intracellular cholesterol contents measured in SHEDs with different treatment. Cells added U18666A (2 µg/mL), an inhibitor specific to cholesterol synthesis, were measured as positive reference.

    U18666A purchased from MedChemExpress. Usage Cited in: Emerg Microbes Infect. 2023 Dec;12(2):2261556.  [Abstract]

    HepG2.2.15 cells were treated with 10 μM dynasore, 2 μM U18666A, or DMSO for 48 hours. The levels of secreted HBsAg in the culture supernatant and intracellular HBsAg in the cell lysate were detected by ELISA.

    U18666A purchased from MedChemExpress. Usage Cited in: Emerg Microbes Infect. 2023 Dec;12(2):2261556.  [Abstract]

    HepG2.2.15 cells were treated with 10 μM dynasore, 2 μM U18666A, or DMSO for 48 hours, and HBcAg was detected by WB.

    U18666A purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2023 Jan:209:105497.  [Abstract]

    Pretreatment with AY9944 (15, 30 µM), U18666A (5, 10 µM), lovastatin and MβCD for 2 h can all significantly reduce the level of EV-A71 VP1 protein in HCT-8 cell.

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    製品説明

    U18666A, an intra-cellular cholesterol transport inhibitor, inhibits replication of Ebola virus, dengue virus, and human hepatitis C virus[1].

    IC50 & Target

    Cholesterol[1][2].
    Cellular Effect
    Cell Line Type Value Description References
    HL-60 IC50
    > 50 μM
    Compound: U18666A
    Cytotoxicity in human HL60 cells by MTT assay
    Cytotoxicity in human HL60 cells by MTT assay
    		[PMID: 28964935]
    Huh-7 CC50
    34.4 μM
    Compound: 67
    Cytotoxicity against human HuH7 cells assessed as cell viability after 48 hrs by CellTiter Glo assay
    Cytotoxicity against human HuH7 cells assessed as cell viability after 48 hrs by CellTiter Glo assay
    		[PMID: 26771861]
    体外実験

    U18666A, the antiviral effect is found to result from two events: retarded viral trafficking in the cholesterol-loaded late endosomes/lysosomes and suppresse de novo sterol biosynthesis in treated infected cells. It is also observed an additive antiviral effect of U18666A with C75, a fatty acid synthase inhibitor, suggesting dengue virus relies on both the host cholesterol and fatty acid biosynthesis for successful replication[1][2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    U18666A 関連抗体
    分子量

    424.06

    分子式

    C25H42ClNO2
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@@]12[C@]3([H])[C@](CC=C1C[C@@H](OCCN(CC)CC)CC2)([H])[C@@]4([H])[C@@](C)(C(CC4)=O)CC3.Cl
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    -20°C, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
    溶剤 & 溶解度
    体外: 

    DMSO : 20.83 mg/mL (49.12 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 5.56 mg/mL (13.11 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.3582 mL 11.7908 mL 23.5816 mL
    5 mM 0.4716 mL 2.3582 mL 4.7163 mL
    10 mM 0.2358 mL 1.1791 mL 2.3582 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

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    体積 （終了）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 5 mg/mL (11.79 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.92%

    COA (247 KB) HNMR (144 KB) RP-HPLC (243 KB) MS (153 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.3582 mL 11.7908 mL 23.5816 mL 58.9539 mL
    5 mM 0.4716 mL 2.3582 mL 4.7163 mL 11.7908 mL
    10 mM 0.2358 mL 1.1791 mL 2.3582 mL 5.8954 mL
    DMSO 15 mM 0.1572 mL 0.7861 mL 1.5721 mL 3.9303 mL
    20 mM 0.1179 mL 0.5895 mL 1.1791 mL 2.9477 mL
    25 mM 0.0943 mL 0.4716 mL 0.9433 mL 2.3582 mL
    30 mM 0.0786 mL 0.3930 mL 0.7861 mL 1.9651 mL
    40 mM 0.0590 mL 0.2948 mL 0.5895 mL 1.4738 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    U18666A Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    U18666A3039-71-2Dengue VirusFlavivirusHCVDENVHepatitis C virusInhibitorinhibitorinhibit

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    製品名:
    U18666A
    製品番号:
    HY-107433
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