1. Antibodies
  2. Primary Antibodies
  3. Polyclonal Antibodies
  4. CXCR2 Antibody

CXCR2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CXCR2.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

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  • Descripciòn

Descripciòn

CXCR2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CXCR2.

Host

Rabbit

Clonality

Polyclonal

Peso molecular
Predicted band size: 41 kDa;
Observed band size: 41 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

The antiserum was produced against synthesized peptide derived from the N-terminal region of human CXCR2.

Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:10000
Sensitivity Endogenous Pureza affinity purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image
WB
  • Western blot analysis of extracts from A549(lane 2(20μg), MDA-MB-231(lane 3(20μg), SK-BR-3(lane 4(20μg) and HepG2(lane 5(20μg) using CXCR2 Antibody (HY-P81158). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993,1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background
Function:Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor (PubMed:1891716). Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed:8662698). Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2
Subcellular Localization:Cell membrane; Multi-pass membrane protein
Subunit:Interacts with IL8 (PubMed:1891716). Interacts with GNAI2 (PubMed:8662698)
RRID
Database
Synonyms
CXC-chemokine receptor 2; CD 182; CD182; CD182 antigen; CDw128b; Chemokine (CXC) receptor 2; CMKAR2; CXCR 2; CXC R2; CXC-R2; CXCR2_HUMAN; C-X-C chemokine receptor type 2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8R B; IL-8 receptor type 2.
Documentación

CXCR2 Antibody Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

Nombre del producto:
CXCR2 Antibody
Cat. No.:
HY-P81158
Cantidad:
MCE Japan Authorized Agent: