2. Antibodies
  3. Primary Antibodies
  4. Polyclonal Antibodies
  5. DLST Antibody

DLST Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to DLST.

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容量 価格（税別） 在庫状況 数量
20 μL $135 在庫あり
50 μL $200 在庫あり
100 μL $325 在庫あり
250 μL   お問い合わせ  
or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • 説明

  • 参考文献

製品説明

DLST Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to DLST.
Host

Rabbit
Clonality

Polyclonal
分子量
Predicted band size: 41 kDa;
Observed band size: 46 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse Predicted Reactivity: Rat, Rabbit, Pig, Sheep, Cow, Dog, Horse
Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
SwissProt ID
Gene ID
Immunogen

KLH conjugated synthetic peptide derived from human DLST: 201-300/453

Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-2000
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:5000-10000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:100-500
IHC-F
IHC-F: Immunohistochemistry-Frozen
1:100-500
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100-500
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100-500
Sensitivity Endogenous 純度 Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from 293T (lane 1) and 3T3 (lane 2) using DLST antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background
Function：Dihydrolipoamide succinyltransferase (E2) component of the 2-oxoglutarate dehydrogenase complex. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion (PubMed:29211711, PubMed:30929736). A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (PubMed:29211711)
Subcellular Localization：Mitochondrion matrix; Nucleus
Isoforms & Post-Translational Modification：P36957 has 2 isomers: P36957-1: 48755 Da (predicted); P36957-2: 39553 Da (predicted).
Subunit：The 2-oxoglutarate dehydrogenase complex is composed of OGDH (2-oxoglutarate dehydrogenase; E1), DLST (dihydrolipoamide succinyltransferase; E2), DLD (dihydrolipoamide dehydrogenase; E3) and the assembly factor KGD4 (By similarity). It contains multiple copies of the three enzymatic components (E1, E2 and E3). In the nucleus, the 2-oxoglutarate dehydrogenase complex associates with KAT2A (PubMed:29211711). Interacts with ABHD11; this interaction maintains the functional lipoylation of the 2-oxoglutarate dehydrogenase complex (PubMed:32792488)
RRID
Database

Entrez Gene: 1743 Human ; 78920 Mouse ;

SwissProt: P36957 Human ; Q9D2G2 Mouse ;

OMIM: 618475 Human

Synonyms
2-oxoglutarate dehydrogenase complex component E2; Dihydrolipoamide S succinyltransferase (E2 component of 2 oxo glutarate complex); Dihydrolipoamide S succinyltransferase; Dihydrolipoamide succinyltransferase component of 2 oxoglutarate dehydrogenase complex; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex; Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex; Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex mitochondrial; Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex; Dlst; DLTS; E2; E2K; mitochondrial; ODO2_HUMAN; OGDC-E2.
ドキュメンテーション

COA (205 KB)

User Guide for Antibodies (1077 KB)
参考文献

DLST Antibody 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
DLST Antibody
製品番号:
HY-P81071
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