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  4. Monoclonal Antibodies Recombinant Antibodies
  5. Glucokinase Antibody (YA2040)

Glucokinase Antibody (YA2040)

Cat. No.: HY-P82295
COA
SDS
User Guide for Antibodies Technical Support

Glucokinase Antibody (YA2040) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucokinase.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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  • Descripciòn

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Descripciòn

Glucokinase Antibody (YA2040) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucokinase.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Peso molecular
Predicted band size: 52 kDa;
Observed band size: 52 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Glucokinase aa250-465/465.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Pureza Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Envío

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis was performed on extracts from BxPC-3 (lane 1, 15 μg), SMMC-7721 (lane 2, 15 μg), HepG2 (lane 3, 15 μg), and SiHa (lane 4, 15 μg) using GCK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:500 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background
Function：Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively) (PubMed:11916951, PubMed:15277402, PubMed:17082186, PubMed:18322640, PubMed:19146401, PubMed:25015100, PubMed:7742312, PubMed:8325892). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant (By similarity). Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues (PubMed:11916951, PubMed:15277402, PubMed:18322640, PubMed:25015100, PubMed:8325892). Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell (By similarity). In pancreas, plays an important role in modulating insulin secretion (By similarity). In liver, helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage (By similarity). Required to provide D-glucose 6-phosphate for the synthesis of glycogen (PubMed:8878425). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:7742312)
Subcellular Localization：Cytoplasm; Nucleus; Mitochondrion
Isoforms & Post-Translational Modification：P35557 has 3 isomers: P35557-1: 52191 Da (predicted); P35557-2: 52136 Da (predicted); P35557-3: 52035 Da (predicted).
Subunit：Monomer (PubMed:15016359, PubMed:19362831, PubMed:23957911). Interacts with MIDN; the interaction occurs preferentially at low glucose levels and results in inhibition of hexokinase activity (PubMed:24187134). Interacts with GCKR; leading to sequestration in the nucleus (PubMed:10456334)
RRID
Database

Entrez Gene: 2645 Human

SwissProt: P35557 Human

OMIM: 125851 Human

Research Field

Signal Transduction
Synonyms
GCK; Glucokinase; Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D
Documentación
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)
Referencias

Glucokinase Antibody (YA2040) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nombre del producto:
Glucokinase Antibody (YA2040)
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HY-P82295
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