Hexokinase II Antibody (YA386)

Cat. No.: HY-P80703: Ficha de datos COA
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Hexokinase II Antibody (YA386) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Hexokinase II.

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Tamaño	Stock	Cantidad
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10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
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Hexokinase II Antibody (YA386) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Descripciòn

Descripciòn

Hexokinase II Antibody (YA386) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Hexokinase II.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Peso molecular

Predicted band size: 102 kDa;

Observed band size: 102 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat, Hamster

SwissProt ID

P52789

Gene ID

3099 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Hexokinase II.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Pureza	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

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ALL WB IHC-P

Western blot analysis of extracts from HL-60 (lane2(20μg), HCT116 (lane3(20μg), THP-1 (lane4(20μg) and HUVEC (lane5(20μg) using Hexokinase II Antibody (HY-P80703). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat testis tissue using Hexokinase II Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat testis tissue using Hexokinase II Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed:23185017, PubMed:26985301, PubMed:29298880). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:29298880). Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed:18350175)

Subcellular Localization：Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasm, cytosol

Expression：
Tissue_specificity:The main hexokinase isoenzyme expressed in insulin-sensitive tissues such as skeletal muscle

Subunit：Monomer (By similarity). Interacts with TIGAR; the interaction increases hexokinase activity in a hypoxia- and HIF1A-dependent manner (PubMed:23185017)

RRID

AB_3102673

Database

Entrez Gene: 3099 Human ; 15277 Mouse ; 25059 Rat

SwissProt: P52789 Human ; O08528 Mouse ; P27881 Rat

OMIM: 601125 Human

Research Field

Signal Transduction

Synonyms

HKII; HXK2

Documentación

Select Batch:

Ficha de datos (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Hexokinase II Antibody (YA386) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Hexokinase II Antibody (YA386)

Hexokinase II Antibody (YA386) Recomendaciones destacadas:

Product Detail

Verification Image

Background

Descripciòn

Hexokinase II Antibody (YA386) Related Classifications

Productos vistos recientemente:

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