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  3. Primary Antibodies
  4. Monoclonal Antibodies Tag Antibodies Conjugated Primary Antibodies
  5. His Tag Antibody (HRP) (YA877)

His Tag Antibody (HRP) (YA877)

Cat. No.: HY-P80947A
COA
SDS
User Guide for Antibodies Technical Support

His Tag Antibody (HRP) (YA877) is a HRP-conjugated, mouse-derived IgG monoclonal antibody, targeting to His Tag.

For research use only. We do not sell to patients.

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50 μL In-stock
100 μL In-stock
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Top Publications Citing Use of Products

1 Publications Citing Use of MCE His Tag Antibody (HRP) (YA877)

  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • Documentation

  • References

Description

His Tag Antibody (HRP) (YA877) is a HRP-conjugated, mouse-derived IgG monoclonal antibody, targeting to His Tag.
Host

Mouse
Clonality

Monoclonal
Molecular Weight
Predicted band size: 1 kDa;
Observed band size: 47 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Species independent
Immunogen

HHHHHH
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:10000
Sensitivity Endogenous Purity Affinity Purified
Conjugation HRP Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from HY-P70499 (lane 2(50 ng), HY-P72468 (lane 3(50 ng), HY-P7993A (lane 4(50 ng) and HY-P70620 (lane 5(50 ng) using His-tag (HRP) (HY-P80947A) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Western blot analysis was performed on extracts from K005-10ng (lane 1, 10ng), K005-5ng (lane 2, 5ng), K005-2ng (lane 3, 2ng), K005-1ng (lane 4, 1ng), K005-0.5ng (lane 5, 0.5ng), and K005-0.1ng (lane 6, 0.1ng) using His Tag Mouse mAb (HRP).Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

Background
Function：His tags are short peptide sequences (usually composed of 6-10 consecutive histidine residues) fused to the N-terminus or C-terminus of a target protein through genetic engineering. They are used for the efficient and rapid purification of recombinant proteins using metal chelate affinity chromatography.
RRID
Research Field

Tags & Cell Markers
Documentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)
References

His Tag Antibody (HRP) (YA877) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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His Tag Antibody (HRP) (YA877)
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