Osteopontin Antibody

Cat. No.: HY-P80771: Data Sheet COA
SDS
User Guide for Antibodies
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Osteopontin Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Osteopontin.

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사이즈	재고	수량
20 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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Osteopontin Antibody Featured Recommendations:

1 Publications Citing Use of MCE Osteopontin Antibody

•Pharmacol Res. 2025 Aug 26:220:107933. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
제품 설명
References

제품 설명

Osteopontin Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Osteopontin.

Host

Rabbit

Clonality

Polyclonal

분자량

Predicted band size: 35 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P10451

Gene ID

6696 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Osteopontin.AA range:261-310.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

ALL WB IHC-P

Western blot analysis of extracts from NIH3T3(lane 2(20μg) and NIH3T3(lane 3(40μg) using Osteopontin (HY-P80771) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from HY-P70499 (lane 2(25 ng) and HY-P70499 (lane 3(50 ng) using Osteopontin (HY-P80771) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using Osteopontin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80771, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using Osteopontin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80771, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction; Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity

Subcellular Localization：Secreted

Expression：
Tissue_specificity:Protein levels were detected in cerebrospinal fluid and urine (PubMed:25326458, PubMed:36213313, PubMed:37453717) . In bone. Found in plasma.

Isoforms & Post-Translational Modification：P10451 has 4 isomers: P10451-1: 35423 Da (predicted); P10451-3: 32355 Da (predicted); P10451-4: 33017 Da (predicted); P10451-5: 33843 Da (predicted).
Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties;Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif (PubMed:15869464, PubMed:22582013, PubMed:26091039). The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization (By similarity);O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62

Subunit：Interacts (via N-terminus) with integrin ITGA9:ITGB1

RRID

AB_3102704

Database

Entrez Gene: 6696 Human ;

SwissProt: P10451 Human ;

OMIM: 166490 Human

Research Field

Cardiovascular

Synonyms

SPP1; BNSP; OPN; PSEC0156; Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin

각종 서류

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/a549-cell.html [Content Brief]

Osteopontin Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Osteopontin Antibody

Osteopontin Antibody Featured Recommendations:

1 Publications Citing Use of MCE Osteopontin Antibody

Product Detail

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Background

제품 설명

References

Osteopontin Antibody Related Classifications

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