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  5. Phospho-Cyclin B1 (Ser126) Antibody (YA208)

Phospho-Cyclin B1 (Ser126) Antibody (YA208)

Art. -Nr.: HY-P80806
COA
SDS
User Guide for Antibodies Technical Support

Phospho-Cyclin B1 (Ser126) Antibody (YA208) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Cyclin B1 (Ser126).

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • Beschreibung

Beschreibung

Phospho-Cyclin B1 (Ser126) Antibody (YA208) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Cyclin B1 (Ser126).
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molekulargewicht
Predicted band size: 48 kDa;
Observed band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser126 of Human Cyclin B1.The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Reinheit affinity purified
Conjugation Non-conjugated Modification Phosphorylated
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Versand

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using Phospho-Cyclin B1 (Ser126) (HY-P80806) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Essential for the control of the cell cycle at the G2/M (mitosis) transition
Subcellular Localization：Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Isoforms & Post-Translational Modification：P14635 has 2 isomers: P14635-1: 48337 Da (predicted); P14635-2: 44102 Da (predicted).
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction (PubMed:16009132). Deubiquitinated by USP22 during G2/M phase (PubMed:27030811);Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site
Subunit：Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization. Interacts with CDK5RAP3 (PubMed:15790566). Interacts with RFPL4A and UBE2A (By similarity). Interacts with INCA1 (PubMed:21540187)
RRID
Database

Entrez Gene: 891 Human

SwissProt: P14635 Human

OMIM: 123836 Human

Research Field

Cell Biology
Synonyms
CCNB1; CCNB; G2/mitotic-specific cyclin-B1
Dokumentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-Cyclin B1 (Ser126) Antibody (YA208) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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