RAB7 Antibody (YA110)

製品番号: HY-P80295: データシート SDS COA User Guide for Antibodies
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RAB7 Antibody (YA110) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAB7.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$84	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

顧客検証

•bioRxiv. 2024 August 07.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

RAB7 Antibody (YA110) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAB7.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 23 kDa;

Observed band size: 23 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P51149

Gene ID

7879 [NCBI]

Immunogen

Synthetic peptide corresponding to Human RAB7.AA range:158-207.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:1000
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:100-1:500
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:1000
FC FC: Flow Cytometry	1:500-1:1000

Sensitivity	Endogenous	純度	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

ALL ICC WB FC

Immunocytochemistry analysis of NIH3T3 cells labeling RAB7 with RAB7 antibody (HY-P80295) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RAB7 antibody (HY-P80295) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of NIH3T3 cells labeling RAB7 with RAB7 antibody (HY-P80295) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RAB7 antibody (HY-P80295) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Western blot analysis of extracts from Hela (lane 2(20μg), C2C12 (lane 3(20μg), C6 (lane 4(20μg) using CD44 (HY-P80295) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80295, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Flow cytometric analysis of 1X10^6 K562 cells labeling RAB7 Antibody (HY-P80295, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

Function：The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:38538795). In its active state, RAB7A binds to a variety of effector proteins playing a key role in the regulation of endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Also plays a central role in growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA (PubMed:11179213, PubMed:12944476, PubMed:14617358, PubMed:20028791, PubMed:21255211). Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (By similarity). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). Required for vesicular trafficking and cell surface expression of ACE2 (PubMed:33147445). May play a role in PRPH neuronal intermediate filament assembly (By similarity)

Subcellular Localization：Cytoplasmic vesicle, phagosome membrane; Peripheral membrane protein; Cytoplasmic side; Late endosome membrane; Peripheral membrane protein; Cytoplasmic side; Lysosome membrane; Peripheral membrane protein; Cytoplasmic side; Melanosome membrane; Peripheral membrane protein; Cytoplasmic side; Cytoplasmic vesicle, autophagosome membrane; Peripheral membrane protein; Cytoplasmic side; Lipid droplet; Endosome membrane; Peripheral membrane protein; Cytoplasmic vesicle; Mitochondrion membrane; Peripheral membrane protein

Expression：
Tissue_specificity:Widely expressed; highly expressed in skeletal muscle

Subunit：The GTP-bound form interacts with RAC1 (By similarity). Interacts with NTRK1/TRKA (By similarity). Interacts with C9orf72 (By similarity). Interacts with CHM, the substrate-binding subunit of the Rab geranylgeranyltransferase complex (By similarity). Interacts with RILP (PubMed:11179213, PubMed:14668488, PubMed:15933719, PubMed:20028791, PubMed:22431521). Interacts with PSMA7 (PubMed:14998988). Interacts with RNF115 (PubMed:16925951). Interacts with FYCO1 (PubMed:20100911). Interacts with the PIK3C3/VPS34-PIK3R4 complex (PubMed:14617358). The GTP-bound form interacts with OSBPL1A (PubMed:16176980). Interacts with CLN3 (PubMed:22261744). Does not interact with HPS4 and the BLOC-3 complex (heterodimer of HPS1 and HPS4) (PubMed:20048159). Interacts with CLN5 (PubMed:22431521). Interacts with PLEKHM1 (via N- and C-terminus) (PubMed:28325809). Interacts with PRPH; the interaction is direct (PubMed:23179371). Interacts with VPS13A (PubMed:30709847). The GDP-bound form interacts with RIMOC1 (PubMed:34432599). Interacts with the MON1A-CCZ1B complex and this interaction is enhanced in the presence of RIMOC1 (PubMed:34432599). Interacts with VPS39 and VPS41 (PubMed:37821429). Interacts with RUFY4 (PubMed:26416964). Forms a ternary complex with LAMP2 and RUFY4; the interaction with LAMP2 is mediated by RUFY4 (via RUN and coiled coil domains) (By similarity)

RRID

AB_3102410

Database

Entrez Gene: 7879 Human ; 19349 Mouse ;

SwissProt: P51149 Human ; P51150 Mouse ;

OMIM: 600882 Human

Research Field

Tags & Cell Markers

Synonyms

CMT2B antibody; PRO2706 antibody; PSN antibody; RAB7, member RAS oncogene family antibody; RAB7A antibody; RAB7A, member RAS oncogene family antibody; RAB7A_HUMAN antibody; Ras associated protein RAB7 antibody; Ras related protein Rab7 antibody; Ras related protein Rab7a antibody

ドキュメンテーション

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データシート (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)