Semaphorin 4D Antibody (YA1922)

Cat. No.: HY-P82177: Ficha de datos COA
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Semaphorin 4D Antibody (YA1922) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Semaphorin 4D.

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Tamaño	Stock	Cantidad
20 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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Semaphorin 4D Antibody (YA1922) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Descripciòn

Descripciòn

Semaphorin 4D Antibody (YA1922) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Semaphorin 4D.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Peso molecular

Predicted band size: 96 kDa;

Observed band size: 120 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q92854

Gene ID

10507 [NCBI]

Immunogen

A synthetic peptide of human Semaphorin 4D aa142-156.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Pureza	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from HL-60 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), Daudi (lane 3, 15 μg), and MCF-7 (lane 4, 15 μg) using SEMA4D Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed:20877282). Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed:19788569). Promotes the migration of cerebellar granule cells (PubMed:16055703). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed:8876214). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed:16055703)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:This gene is highly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus, and also expressed at lower levels in the testes, brain, kidneys, small intestine, prostate, heart, placenta, lungs, and pancreas, but is not expressed in the colon and liver.

Isoforms & Post-Translational Modification：Q92854 has 2 isomers: Q92854-1: 96150 Da (predicted); Q92854-2: 82164 Da (predicted).

Subunit：Homodimer (PubMed:20877282). Interacts with PLXNB1 (PubMed:10520995)

Database

Entrez Gene: 10507 Human

SwissProt: Q92854 Human

OMIM: 601866 Human

Research Field

Immunology

Synonyms

CD100; COLL4; SEMAJ; coll-4; C9orf164; M-sema-G; A8 antibody; BB 18 antibody; Coll 4 antibody; COLL4 antibody; Collapsin 4 antibody; GR3 antibody; M sema G antibody; MSEMA antibody; Sema 4d antibody; Sema H antibody; SEMA J antibody; Sema4d antibody; SEMAJ antibody; Semaphorin 4D antibody; Semaphorin C like 2 antibody; Semaphorin H antibody; Semaphorin J antibody; Semaphorin-4D antibody; SemaphorinJ antibody; Semcl 2 antibody; Semcl2 antibody

Documentación

Select Batch:

Ficha de datos (234 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Semaphorin 4D Antibody (YA1922) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Semaphorin 4D Antibody (YA1922)

Semaphorin 4D Antibody (YA1922) Recomendaciones destacadas:

Product Detail

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Background

Descripciòn

Semaphorin 4D Antibody (YA1922) Related Classifications

Productos vistos recientemente:

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