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  5. TERT Antibody

TERT Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to TERT.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Description

  • Références

Description

TERT Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to TERT.
Host

Rabbit
Clonality

Polyclonal
Masse moléculaire
Predicted band size: 125 kDa;
Observed band size: 125 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat,
SwissProt ID
Gene ID
Immunogen

KLH conjugated synthetic peptide derived from human TERT: 601-750/1132
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-2000
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:5000-10000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:100-500
IHC-F
IHC-F: Immunohistochemistry-Frozen
1:100-500
FC
FC: Flow Cytometry
1μg/Test
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100-500
Sensitivity Endogenous Pureté affinity purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Livraison

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from HeLa(lane 2(20μg), K562(lane 3(20μg), Mouse Lung(lane 4(20μg) and Rat Lung(lane 5(20μg) using TERT Antibody (HY-P81110). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

Background
Function：Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis
Subcellular Localization：Nucleus, nucleolus; Nucleus, nucleoplasm; Nucleus; Chromosome, telomere; Cytoplasm; Nucleus, PML body
Expression：
Tissue_specificity:It is highly expressed in thymocyte subsets, moderately expressed in tonsillar T lymphocytes, and lowly expressed or undetectable in peripheral blood T lymphocytes.

Induction:Activated by cytotoxic events and down-regulated during aging. In peripheral T-lymphocytes, induced By CD3 and by PMA/ionomycin. Inhibited by herbimycin B
Isoforms & Post-Translational Modification：O14746 has 4 isomers: O14746-1: 126997 Da (predicted); O14746-2: 90226 Da (predicted); O14746-3: 120048 Da (predicted); O14746-4: 88965 Da (predicted).
Phosphorylation at Tyr-707 under oxidative stress leads to translocation of TERT to the cytoplasm and reduces its antiapoptotic activity. Dephosphorylated by SHP2/PTPN11 leading to nuclear retention. Phosphorylation at Ser-227 by the AKT pathway promotes nuclear location. Phosphorylation at the G2/M phase at Ser-457 by DYRK2 promotes ubiquitination by the EDVP complex and degradation;Ubiquitinated by the EDVP complex, a E3 ligase complex following phosphorylation at Ser-457 by DYRK2. Ubiquitinated leads to proteasomal degradation;(Microbial infection) In case of infection by HIV-1, the EDVP complex is hijacked by HIV-1 via interaction between HIV-1 Vpr and DCAF1/VPRBP, leading to ubiquitination and degradation
Subunit：Catalytic component of the telomerase holoenzyme complex composed of one molecule of TERT, one molecule of WRAP53/TCAB1, two molecules of H/ACA ribonucleoprotein complex subunits DKC1, NOP10, NHP2 and GAR1, and a telomerase RNA template component (TERC) (PubMed:19179534, PubMed:20351177, PubMed:29695869).
RRID
Database

Entrez Gene: 7015 Human ; 21752 Mouse ; 301965 Rat

SwissProt: O14746 Human ; O70372 Mouse ; Q673L6 Rat

OMIM: 609135 Human

Synonyms
Telomerase catalytic subunit; EST2; hEST2; TCS1; Telomerase associated protein 2; Telomere Reverse Transcriptase; TP2; TRT; Telomerase reverse transcriptase; Telomerase Catalytic Subunit; Telomerase-associated protein 2; TERT_HUMAN.
Documentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)
Références

TERT Antibody Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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