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  5. Transferrin Receptor Antibody (YA3546)

Transferrin Receptor Antibody (YA3546)

Cat. No.: HY-P81000
COA
SDS
User Guide for Antibodies Technical Support

Transferrin Receptor Antibody (YA3546) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transferrin Receptor.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • 제품 설명

  • References

제품 설명

Transferrin Receptor Antibody (YA3546) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transferrin Receptor.
Host

Rabbit
Clonality

Monoclonal,Recombinant
분자량
Predicted band size: 85 kDa;
Observed band size: 90 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human TFR.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
IP
IP: Immunoprecipitation
1:50
FC
FC: Flow Cytometry
1:50-1:100
Sensitivity Endogenous Conjugation Non-conjugated
Modification Unmodified Isotype IgG
Appearance

Liquid
Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
ALL WB IHC-P

  • Western blot analysis of extracts from K562(lane2(20μg), Jurkat (lane3(20μg), Hela (lane4(20μg) and HepG2 (lane5(20μg) using Transferrin Receptor Antibody (HY-P81000). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Transferrin Receptor 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Transferrin Receptor 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background
Function：Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738). Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the hereditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738). When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (PubMed:26214738). When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (PubMed:26214738). Mediates uptake of NICOL1 into fibroblasts where it may regulate extracellular matrix production (By similarity); (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus; (Microbial infection) Acts as a host entry factor for rabies virus that hijacks the endocytosis of TFRC to enter cells; (Microbial infection) Acts as a host entry factor for SARS-CoV, MERS-CoV and SARS-CoV-2 viruses that hijack the endocytosis of TFRC to enter cells
Subcellular Localization：Cell membrane; Single-pass type II membrane protein; Melanosome; Secreted
Expression：
Induction:Regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation
Subunit：Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with STEAP3
RRID
Database

Entrez Gene: 7037 Human ; 22042 Mouse ; 64678 Rat

SwissProt: P02786 Human ; Q62351 Mouse ; Q99376 Rat

OMIM: 616740 Human

Synonyms
CD71 antibody; IMD46 antibody; OTTHUMP00000208523 antibody; p90 antibody; sTfR antibody; T9 antibody; TFR 1 antibody; TfR antibody; TFRC antibody; TR antibody; Transferrin receptor (p90 CD71) antibody
각종 서류
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)
References

Transferrin Receptor Antibody (YA3546) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Transferrin Receptor Antibody (YA3546)
Cat. No.:
HY-P81000
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