AUDA
Based on 3 publication(s) in Google Scholar
AUDA (compound 43) is a potent soluble epoxide hydrolase (sEH) inhibitor with IC50s of 18 and 69 nM for the mouse and human sEH, respectively. AUDA has anti-inflammatory activity.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 479413-70-2
- Formula: C23H40N2O3
- Molecular Weight:392.58
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) AUDA
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Biological Activity
IC50: 18 nM (mouse sEH) and 69 nM (human sEH)[1]
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Cell Line
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Type | Value | Description | References |
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| BTI-TN-5B1-4 | IC50 |
16 nM
Compound: Table4, R12
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Inhibition of recombinant human sEH expressed in insect High Five cells preincubated for 5 mins followed by addition of t-DPPO as substrate measured after 10 mins by liquid scintillation counter method
Inhibition of recombinant human sEH expressed in insect High Five cells preincubated for 5 mins followed by addition of t-DPPO as substrate measured after 10 mins by liquid scintillation counter method
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[PMID: 27120257] |
AUDA (0.3-10 μg/mL; 48 hours) dose-dependently suppresses the proliferation of rat VSMCs exposed to PDGF[2].
AUDA (0.3-10 μg/mL; 30 min) dose-dependently upregulats COX-2 expression[2].
AUDA (10, 50 and 100 μM) augments the migratory ability of HCAECs in a dose-dependent manner[3].
AUDA significantly increases the adhesion ability of HCAECs[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Vascular smooth muscle cell (VSMC)
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Concentration:0.3, 1, 3, 10 μg/mL
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Incubation Time:48 hours
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Result:Dose-dependently suppressed the proliferation of rat VSMCs exposed to PDGF.
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Cell Line:VSMC
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Concentration:1, 3, 10, 30 μg/mL
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Incubation Time:30 min
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Result:Dose-dependently upregulated COX-2 expression.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male (wild-type) C57BL/6 mice (age, 4-6 weeks; weight, 18-20 g)[3]
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Dosage:10 mg/kg
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Administration:i.p.; 14 days
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Result:Reduced TNF-α, MMP-9 and IL-1β expression levels.
Chemical Information
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CAS No. 479413-70-2
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Appearance Solid
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Molecular Weight 392.58
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Formula C23H40N2O3
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Color White to off-white
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SMILES
O=C(O)CCCCCCCCCCCNC(NC1(C2)CC3CC2CC(C3)C1)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (3)
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Journal Impact Factor
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Most Recent
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J Mol Cell Cardiol
Glimepiride, a novel soluble epoxide hydrolase inhibitor, protects against heart failure via increasing epoxyeicosatrienoic acids. [Abstract]2023 Dec:185:13-25. PMID: 37871528 -
J Neuroimmunol
Effect of the sEH inhibitor AUDA on arachidonic acid metabolism and NF-κB signaling of rats with postpartum depression-like behavior. [Abstract]2023 Dec 15:385:578250. PMID: 38029646 -
Heliyon
Effect of Shenqi Jieyu formula on inflammatory response pathway in hippocampus of postpartum depression rats. [Abstract]2024 Apr 30;10(9):e29978. PMID: 38726147
Solvent & Solubility
DMSO : 125 mg/mL (318.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (5.30 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.30 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1]. Morisseau C, et al. Structural refinement of inhibitors of urea-based soluble epoxide hydrolases.Biochem Pharmacol. 2002 May 1;63(9):1599-608. [Content Brief]
[2]. Kim HS, et al. Differential Effects of sEH Inhibitors on the Proliferation and Migration of Vascular Smooth Muscle Cells.Int J Mol Sci. 2017 Dec 11;18(12). [Content Brief]
[3]. Dai N, et al. Vascular repair and anti-inflammatory effects of soluble epoxide hydrolase inhibitor.Exp Ther Med. 2019 May;17(5):3580-3588. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5473 mL | 12.7363 mL | 25.4725 mL | 63.6813 mL |
| 5 mM | 0.5095 mL | 2.5473 mL | 5.0945 mL | 12.7363 mL | |
| 10 mM | 0.2547 mL | 1.2736 mL | 2.5473 mL | 6.3681 mL | |
| 15 mM | 0.1698 mL | 0.8491 mL | 1.6982 mL | 4.2454 mL | |
| 20 mM | 0.1274 mL | 0.6368 mL | 1.2736 mL | 3.1841 mL | |
| 25 mM | 0.1019 mL | 0.5095 mL | 1.0189 mL | 2.5473 mL | |
| 30 mM | 0.0849 mL | 0.4245 mL | 0.8491 mL | 2.1227 mL | |
| 40 mM | 0.0637 mL | 0.3184 mL | 0.6368 mL | 1.5920 mL | |
| 50 mM | 0.0509 mL | 0.2547 mL | 0.5095 mL | 1.2736 mL | |
| 60 mM | 0.0425 mL | 0.2123 mL | 0.4245 mL | 1.0614 mL | |
| 80 mM | 0.0318 mL | 0.1592 mL | 0.3184 mL | 0.7960 mL | |
| 100 mM | 0.0255 mL | 0.1274 mL | 0.2547 mL | 0.6368 mL |
AUDA Related Classifications
HY-108570 Related Classifications
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