2. Apoptosis GPCR/G Protein
  3. Arrestin Apoptosis
  4. Barbadin

Barbadin is a novel and selective β-arrestin/β2-adaptin interaction inhibitor, has IC50 values of 19.1 μM for β-arrestin1 and 15.6 μM for β-arrestin2. Barbadin blocks agonist-promoted endocytosis of the prototypical β2-adrenergic, V2-vasopressin and angiotensin-II type-1 receptors. Barbadin can induce apoptosis.

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CAS 番号 : 356568-70-2

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 211 在庫あり
Solution
10 mM * 1 mL in DMSO USD 211 在庫あり
Solid
1 mg $65 在庫あり
2 mg $114 在庫あり
5 mg $192 在庫あり
10 mg $307 在庫あり
25 mg $645 在庫あり
50 mg $1032 在庫あり
100 mg $1650 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 16 publication(s) in Google Scholar

Barbadin 関連抗体

Top Publications Citing Use of Products

    Barbadin purchased from MedChemExpress. Usage Cited in: Neuron. 2025 Nov 26:S0896-6273(25)00842-6.  [Abstract]

    Barbadin (100 μM; 2 h) was used to inhibit β-arrestin signaling and could indeed eliminate the inhibitory effect of MF-8 on LTP.

    Barbadin purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2024 Mar 11:18:781-799.

    Barbadin (50μM) inhibited β-arr2 expression compared with the blank control, together with the inhibition of SRECA2a and SUMO1 protein expression, and blocked the effect of QFYXF, which upregulated SERCA2a and SUMO1 expression in AngII-induced hypertrophic NRCMs.

    Barbadin purchased from MedChemExpress. Usage Cited in: Sci Signal. 2023 Apr 4;16(779):eabl4283.  [Abstract]

    Migration of sDCs toward 100 nM 7α,25-OHC upon preincubation with DMSO (vehicle) or Barbadin (50-100 μM; 30 min). Barbadin significantly decreased the migration of sDCs toward 7α,25-OHC in a concentration-dependent manner.

    Barbadin purchased from MedChemExpress. Usage Cited in: Sci Signal. 2023 Apr 4;16(779):eabl4283.  [Abstract]

    Analysis of 7α,25-OHC-induced signaling by GPR183 upon preincubation with either DMSO (vehicle) or Barbadin (50 μM; 1 h). Barbadin decreased ligand-induced β-arrestin1 and β-arrestin2 recruitment to GPR183 but not its G protein signaling.

    Barbadin purchased from MedChemExpress. Usage Cited in: Cell Chem Biol. 2022 Jan 20;29(1):67-73.e3.  [Abstract]

    The β-arrestin inhibitor Barbadin (100 μM; 30 min) prolongs PLD activity downstream of PTHR1–Gq signaling in hPTHR1-293 cells.

    Arrestin アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Arrestin-2/β-Arrestin 1 Arrestin-3/β-Arrestin 2

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Barbadin is a novel and selective β-arrestin/β2-adaptin interaction inhibitor, has IC50 values of 19.1 μM for β-arrestin1 and 15.6 μM for β-arrestin2. Barbadin blocks agonist-promoted endocytosis of the prototypical β2-adrenergic, V2-vasopressin and angiotensin-II type-1 receptors. Barbadin can induce apoptosis[1][2].

    IC50 & Target

    Arrestin-3/β-Arrestin 2

     

    体外実験

    Barbadin (4 h) treatment reduces cell viability and induces apoptosis[2].
    Barbadin (2 h) treatment arrests breast cancer cells in G0/G1 phase[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Barbadin 関連抗体

    Apoptosis Analysis[2]

    Cell Line: MDA MB-231 cells
    Concentration:
    Incubation Time: 4 hours
    Result: Exhibited morphological characteristics of apoptosis including shrinkage, rounding and detachment, the percent of cell viability was reduced to 69.1% and apoptosis was developed in 29.9% of cells starved with EBSS (Earle’s balanced salt solution).

    Cell Cycle Analysis[2]

    Cell Line: MDA MB-231 cells
    Concentration:
    Incubation Time: 2 hours
    Result: Arrested 63.7% of cells in G0/G1 phase.
    分子量

    333.41

    分子式

    C19H15N3OS
    CAS 番号
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O=C1C(C(C2=CC=C(CC3=CC=CC=C3)C=C2)=CS4)=C4N=CN1N
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (149.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.9993 mL 14.9966 mL 29.9931 mL
    5 mM 0.5999 mL 2.9993 mL 5.9986 mL
    10 mM 0.2999 mL 1.4997 mL 2.9993 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (7.50 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (7.50 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
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    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 98.93%

    COA (254 KB) HNMR (262 KB) LCMS (281 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.9993 mL 14.9966 mL 29.9931 mL 74.9828 mL
    5 mM 0.5999 mL 2.9993 mL 5.9986 mL 14.9966 mL
    10 mM 0.2999 mL 1.4997 mL 2.9993 mL 7.4983 mL
    15 mM 0.2000 mL 0.9998 mL 1.9995 mL 4.9989 mL
    20 mM 0.1500 mL 0.7498 mL 1.4997 mL 3.7491 mL
    25 mM 0.1200 mL 0.5999 mL 1.1997 mL 2.9993 mL
    30 mM 0.1000 mL 0.4999 mL 0.9998 mL 2.4994 mL
    40 mM 0.0750 mL 0.3749 mL 0.7498 mL 1.8746 mL
    50 mM 0.0600 mL 0.2999 mL 0.5999 mL 1.4997 mL
    60 mM 0.0500 mL 0.2499 mL 0.4999 mL 1.2497 mL
    80 mM 0.0375 mL 0.1875 mL 0.3749 mL 0.9373 mL
    100 mM 0.0300 mL 0.1500 mL 0.2999 mL 0.7498 mL
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    Barbadin Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Barbadin356568-70-2ArrestinApoptosisendocytosisV2-vasopressinangiotensin-II type-1MDA MB-231 cell,Inhibitorinhibitorinhibit

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    製品名:
    Barbadin
    製品番号:
    HY-119706
    数量:
    MCE 日本正規代理店:

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