2. Metabolic Enzyme/Protease Apoptosis NF-κB Immunology/Inflammation
  3. Angiotensin-converting Enzyme (ACE) Apoptosis Reactive Oxygen Species
  4. Benazepril

Benazepril (CGS14824A free base) is an orally active angiotensin-converting enzyme (ACE) inhibitor to reduce angiotensin-II production. Benazepril inhibits oxidative stress and inhibits apoptosis by the PI3K/Akt signaling pathway. Benazepril improves diabetic nephropathy and decreases proteinuria. Benazepril can be used in the study of hypertension, heart failure and diabetic nephropathy.

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Benazepril Chemical Structure

Benazepril Chemical Structure

CAS No. : 86541-75-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
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25 mg In-stock
50 mg In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Benazepril:

Benazepril Related Antibodies

Top Publications Citing Use of Products

2 Publications Citing Use of MCE Benazepril

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Benazepril (CGS14824A free base) is an orally active angiotensin-converting enzyme (ACE) inhibitor to reduce angiotensin-II production. Benazepril inhibits oxidative stress and inhibits apoptosis by the PI3K/Akt signaling pathway. Benazepril improves diabetic nephropathy and decreases proteinuria. Benazepril can be used in the study of hypertension, heart failure and diabetic nephropathy[1][2].

Cellular Effect
Cell Line Type Value Description References
A549 IC50
358 μM
Compound: Benazepril
Cytotoxicity against human A549 cells assessed as cell survival after 24 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as cell survival after 24 hrs by MTT assay
[PMID: 23777825]
HEK293 IC50
301 μM
Compound: Benazepril
Cytotoxicity against HEK293 cells assessed as cell survival after 24 hrs by MTT assay
Cytotoxicity against HEK293 cells assessed as cell survival after 24 hrs by MTT assay
[PMID: 23777825]
In Vitro

Benazepril (1 μM; 1 h) alleviates oxidative stress and inhibits cell apoptosis through PI3K/Akt signaling pathway in Adriamycin (HY-15142A) treated H9c2 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Benazepril Related Antibodies

Western Blot Analysis[1]

Cell Line: Adriamycin (HY-15142A) treated H9c2 cells.
Concentration: 1 μM
Incubation Time: 1 h
Result: Increased the phosphorylation level of Akt.
In Vivo

Benazepril (10 mg/kg; oral gavage; 16 weeks) has an improved effect in diabetic nephropathy rat model[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Streptozotocin (HY-13753) treated Wistar male rats aged 6-8 weeks old (170-200 g)[2].
Dosage: 10 mg/kg
Administration: Oral gavage (i.g.); 16 weeks
Result: Decreased proteinuria, urea, creatinine, triglycerides and total cholesterol levels.
Increased the albumin level.
Diminished kidney volume and ameliorated the pathological changes associated with diabetic nephropathy.
Significantly decreased ANGPTL-4 expression.
Clinical Trial
Molecular Weight

424.49

Formula

C24H28N2O5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1N(CC(O)=O)C2=CC=CC=C2CC[C@@H]1N[C@@H](CCC3=CC=CC=C3)C(OCC)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (235.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3558 mL 11.7788 mL 23.5577 mL
5 mM 0.4712 mL 2.3558 mL 4.7115 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (5.89 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (5.89 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3558 mL 11.7788 mL 23.5577 mL 58.8942 mL
5 mM 0.4712 mL 2.3558 mL 4.7115 mL 11.7788 mL
10 mM 0.2356 mL 1.1779 mL 2.3558 mL 5.8894 mL
15 mM 0.1571 mL 0.7853 mL 1.5705 mL 3.9263 mL
20 mM 0.1178 mL 0.5889 mL 1.1779 mL 2.9447 mL
25 mM 0.0942 mL 0.4712 mL 0.9423 mL 2.3558 mL
30 mM 0.0785 mL 0.3926 mL 0.7853 mL 1.9631 mL
40 mM 0.0589 mL 0.2945 mL 0.5889 mL 1.4724 mL
50 mM 0.0471 mL 0.2356 mL 0.4712 mL 1.1779 mL
60 mM 0.0393 mL 0.1963 mL 0.3926 mL 0.9816 mL
80 mM 0.0294 mL 0.1472 mL 0.2945 mL 0.7362 mL
100 mM 0.0236 mL 0.1178 mL 0.2356 mL 0.5889 mL
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Benazepril Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Benazepril86541-75-5CGS14824AAngiotensin-converting Enzyme (ACE)ApoptosisReactive Oxygen Speciesdiabetic nephropathy modelACEoral gavageangiotensin-IIInhibitorinhibitorinhibit

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